FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8783449
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8572459.759776598008369No Hit
CCCCCCACTGCTAAATTTGACTG5654526.4376989039271475No Hit
AACATTCAACGCTGTCGGTGAGT3755384.2755186487677No Hit
AAGCTGCCAGTTGAAGAACTGT3347623.8112818779957625No Hit
AACATTCAACGCTGTCGGTGAG3160693.5984611511947073No Hit
TAGCAGCACGTAAATATTGGCG2717013.093329283291791No Hit
TTCAAGTAATCCAGGATAGGCT2419422.7545216007971356No Hit
CCCCCACTGCTAAATTTGACTG2094872.3850198253556205No Hit
TAGCTTATCAGACTGATGTTGA1260571.4351651612026208No Hit
TAGCTTATCAGACTGATGTTGACT1209691.3772380302999425No Hit
TATTGCACTTGTCCCGGCCTGTAA1200051.366262842762564No Hit
TAGCTTATCAGACTGATGTTGAC1199091.3651698780285513No Hit
TATTGCACTTGTCCCGGCCTGT1000191.1387212472002741No Hit
TGAGGTAGTAGGTTGTATAGTT917351.0444074986944194No Hit
TATTGCACTTGTCCCGGCCTGTAT899571.0241648810165573No Hit
CATAAAGTAGAAAGCACTACT841900.9585073016306009No Hit
CGCCTGTCTGAGCGTCGCT800910.911839984498117No Hit
TATTGCACTTGTCCCGGCCTGTA575650.6553803636817382No Hit
TGTAAACATCCTTGACTGGAAGCT560970.6386671112907926No Hit
CCCATAAAGTAGAAAGCACT529950.6033506883230039No Hit
AGCAGCATTGTACAGGGCTATGA476600.5426114502401049No Hit
TGAGGTAGTAGATTGTATAGTT458800.5223460624636176No Hit
CAACACCAGTCGATGGGCTGT413680.470976719965016No Hit
TTCAAGTAATTCAGGATAGGTT385320.43868872011438786No Hit
CAACGGAATCCCAAAAGCAGCTGT382070.43498857908778205No Hit
CATTGCACTTGTCTCGGTCTGA349900.39836287544904053No Hit
TGTAAACATCCCCGACTGGAAGCT347630.3957784692550728No Hit
ACCGGGTGCTGTAGGCTT328270.37373701378581464No Hit
TTAATGCTAATCGTGATAGGGGTT314440.35799149058644275No Hit
CCCATAAAGTAGAAAGCACTAC304760.34697076285181366No Hit
TCGTACGACTCTTAGCGG296180.3372023905415743No Hit
CAACGGAATCCCAAAAGCAGCT282810.3219805796105835No Hit
ACTGGACTTGGAGTCAGAAGGC278650.317244399096528No Hit
CACCCGGCTGTGTGCACATGTGC258920.29478169680270244No Hit
CTACGCCTGTCTGAGCGTCGCT242690.2763037617682985No Hit
AACATTCAACGCTGTCGGTGAGTT236050.26874408902471003No Hit
TGAGGTAGTAGTTTGTACAGTT222880.2537499790799719No Hit
ACGCCTGTCTGAGCGTCGCT212390.24180706235101954No Hit
TACGCCTGTCTGAGCGTCGCT206490.23508988325656585No Hit
TGTCAGTTTGTCAAATACCCCAA203380.23154913292033688No Hit
ACCGGGTGCTGTAGGCTTT196430.2236365236480567No Hit
ACCGGGTGCTGTAGGCTTA185290.21095357871378315No Hit
TTCTCACTACTGCACTTGACTAG176310.2007298044310384No Hit
AACGGAATCCCAAAAGCAGCTG175920.20028578750784573No Hit
GCGTTCGCGCTTTCCCCTG168260.19156483973436858No Hit
ATCCCGGACGAGCCCCCA166220.1892422896745914No Hit
TGAGGTAGTAGTTTGTGCTGTT162930.185496608450735No Hit
TAGCAGCACGTAAATATTGGC159270.1813296804023112No Hit
TGTAAACATCCTACACTCTCAGCT158670.18064657744355322No Hit
TTCACAGTGGCTAAGTTCCG153850.17515898367486393No Hit
GCCTGTCTGAGCGTCGCT149240.16991047594173997No Hit
TATTGCACTTGTCCCGGCCTGTT144380.16437734197580017No Hit
TGATGTAGTAGGTTGTATAGTT141870.1615196945983292No Hit
TAGCTTATCAGACTGATGTTGACA141780.1614172291545155No Hit
TTCACAGTGGCTAAGTTCTG140230.1596525465110573No Hit
ATCCCACCGCTGCCACCA133470.15195625317571718No Hit
TGAGAACTGAATTCCATGGGTT132900.1513073053648971No Hit
TATTGCACTTGTCCCGGCCTGTAG131700.1499410994473811No Hit
ACCACAGGGTAGAACCACGGAC130530.14860904867780297No Hit
TCGTTTCCCGGCCAATGCACCA128650.14646865940702794No Hit
TTCAAGTAATCCAGGATAGGC127690.14537569467301512No Hit
CCCCCCACTGCTAAATTTGACTGG127210.14482921230600873No Hit
CAAAGAATTCTCCTTTTGGGCT118070.13442327723426187No Hit
TTCACAGTGGCTAAGTTCCGC114350.13018803888996225No Hit
TGAGGTAGTAGGTTGTGTGGTT113460.12917476950113788No Hit
CTTCTCACTACTGCACTTGACTAG111830.12731900646317865No Hit
CCCCCACTGCTAAATTTGACTGGCTT110820.12616911648260268No Hit
TTCAAGTAATCCAGGATAGGCTT110510.12581617995391106No Hit
AGCAGCATTGTACAGGGCTATGT110150.12540631817865625No Hit
TTATAAAGCAATGAGACTGATT109520.12468906007196034No Hit
CCCATAAAGTAGAAAGCACTA108820.12389210662007602No Hit
CGCGACCTCAGATCAGAC107690.12260559604774844No Hit
CAAAGTGCTGTTCGTGCAGGTAG104370.11882575967595418No Hit
TGTCAGTTTGTCAAATACCCCA103760.11813127166788354No Hit
AGCAGCATTGTACAGGGCTATG103750.11811988661857091No Hit
CAAAGTGCTGTTCGTGCAGGT103570.11791495573094352No Hit
ACCCCCCACTGCTAAATTTGACTG102810.11704969198318338No Hit
AACATTCAACGCTGTCGGTGA102650.11686753119418124No Hit
TGTAAACATCCCCGACTGGAAGC98290.11190364969387309No Hit
CGCGACCTCAGATCAGACGTGG95760.10902323221777686No Hit
TTCACAGTGGCTAAGTTCTGC93020.10590372870611534No Hit
TGAGGTAGTAGGTTGTATAGT90470.10300054113139383No Hit
CTCCCACTGCTTCACTTGACTAG90050.10252236906026324No Hit
ACTGGACTTGGAGTCAGAAGGCT88910.10122447343862302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)