FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9539764
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG109546711.483166669531867No Hit
CAACGGAATCCCAAAAGCAGCTG6274936.5776574766419795No Hit
AACATTCAACGCTGTCGGTGAGT4167744.368808284984828No Hit
CCCCCACTGCTAAATTTGACTG3052913.2001944702195986No Hit
AAGCTGCCAGTTGAAGAACTGT2993243.137645753081523No Hit
TTCAAGTAATCCAGGATAGGCT2663642.7921445436176406No Hit
AACATTCAACGCTGTCGGTGAG2660312.7886538912283365No Hit
TAGCAGCACGTAAATATTGGCG2512542.633754881148003No Hit
TATTGCACTTGTCCCGGCCTGTAA1450111.5200690499261826No Hit
TAGCTTATCAGACTGATGTTGACT1340051.40469931960581No Hit
TGAGGTAGTAGGTTGTATAGTT1108341.161810711459948No Hit
TATTGCACTTGTCCCGGCCTGTAT1090071.1426592942970077No Hit
TAGCTTATCAGACTGATGTTGAC975281.0223313700422778No Hit
TAGCTTATCAGACTGATGTTGA941200.9866072158598473No Hit
TATTGCACTTGTCCCGGCCTGT685090.7181414550716348No Hit
TGAGGTAGTAGATTGTATAGTT653550.6850798405495147No Hit
TGTAAACATCCTTGACTGGAAGCT645110.6762326615207672No Hit
GGAATACCGGGTGCTGTAGGCTTT553240.5799304888464746No Hit
TATTGCACTTGTCCCGGCCTGTA487790.5113229216152517No Hit
TGTAAACATCCCCGACTGGAAGCT486420.5098868273890214No Hit
TTCTCACTACTGCACTTGACTAG446980.46854408557695976No Hit
TTAATGCTAATCGTGATAGGGGTT406200.4257966968574904No Hit
CATAAAGTAGAAAGCACTACT404010.42350104258344334No Hit
CAACGGAATCCCAAAAGCAGCTGT400020.41931854918004263No Hit
AGCAGCATTGTACAGGGCTATGA396950.4161004402205338No Hit
GGAATACCGGGTGCTGTAGGCTT382450.40090090278962875No Hit
CTTCTCACTACTGCACTTGACTAG368430.38620452245988474No Hit
CCCATAAAGTAGAAAGCACT355080.37221046558384463No Hit
ACTGGACTTGGAGTCAGAAGGC329260.34514480651722623No Hit
CCCCCCACTGCTAAATTTGACTGG319250.33465188447009797No Hit
AACATTCAACGCTGTCGGTGAGTT282620.29625470818774974No Hit
CGCCTGTCTGAGCGTCGCT275580.28887507070405516No Hit
CCCATAAAGTAGAAAGCACTAC274230.2874599413570399No Hit
CCCCCACTGCTAAATTTGACTGGCTT260850.2734344371621772No Hit
ACCGGGTGCTGTAGGCTT257100.2695035223093569No Hit
TGAGGTAGTAGTTTGTACAGTT242620.25432494975766695No Hit
ACCGGGTGCTGTAGGCTTA239980.2515575857012815No Hit
TCGTACGACTCTTAGCGG237870.24934579094409462No Hit
TTCAAGTAATTCAGGATAGGTT230390.24150492611766916No Hit
CTCCCACTGCTTCACTTGACTAG228920.23996400749536365No Hit
CAAAGAATTCTCCTTTTGGGCT228450.23947133283381017No Hit
TGTAAACATCCTACACTCTCAGCT221910.2326158173304916No Hit
AGCTCGGTCTGAGGCCCCTCAGT213510.22381056806017424No Hit
ACCCCCCACTGCTAAATTTGACTG202600.21237422644836915No Hit
AACATTCAACGCTGTCGGTGA198980.20857958331044665No Hit
GACCCGCGGGCGCTCTCCAGTCCT195350.20477445773291664No Hit
GCCCCCACTGCTAAATTTGACTG194300.203673801574127No Hit
CCCCCCACTGCTAAATTTGACTGGCT191940.2011999458267521No Hit
ACCGGGTGCTGTAGGCTTT189530.19867367788133963No Hit
TGAGGTAGTAGTTTGTGCTGTT189300.19843258177036663No Hit
CATTGCACTTGTCTCGGTCTGA180150.18884114952948522No Hit
CGCGACCTCAGATCAGACGTGGCG177290.18584317180173432No Hit
TATTGCACTTGTCCCGGCCTGTAG176510.18502554151234768No Hit
CAACACCAGTCGATGGGCTGT164190.1721111759158822No Hit
AACATTCAACGCTGTCGGTGAGA157240.1648258803886553No Hit
AACGGAATCCCAAAAGCAGCTG150320.157572032180251No Hit
TGAATACCGGGTGCTGTAGGCTTT149540.1567544018908644No Hit
GGGAATACCGGGTGCTGTAGGCTT142890.14978357955186314No Hit
CCCCCCACTGCTAAATTTGACTGT139170.14588411201786544No Hit
TTCACAGTGGCTAAGTTCCG134250.14072675173096527No Hit
CCCCCCACTGCTAAATTTGACTGTA134210.14068482197253518No Hit
CACCCGGCTGTGTGCACATGTGC133860.14031793658627195No Hit
TAGCTTATCAGACTGATGTTGACA132660.13906004383336948No Hit
CTACGCCTGTCTGAGCGTCGCT127820.13398654306332944No Hit
TATTGCACTTGTCCCGGCCTGTT125850.13192150246064788No Hit
TGAGAACTGAATTCCATGGGTT124540.13054830287206268No Hit
GAATACCGGGTGCTGTAGGCTTT123790.1297621199014986No Hit
TTCACAGTGGCTAAGTTCTG122860.1287872530179992No Hit
AACCGGGCGGAAACACCA120220.12601988896161373No Hit
TGAGGTAGTAGGTTGTGTGGTT119020.12476199620871124No Hit
CAAAGAATTCTCCTTTTGGGCTT118600.12432173374519538No Hit
ATCCCGGACGAGCCCCCA118180.12388147128167952No Hit
ACTGGACTTGGAGTCAGAAGGCT115380.1209463881915737No Hit
TTCAAGTAATCCAGGATAGGC114370.11988766179121411No Hit
GGGAATACCGGGTGCTGTAGGCTTT114360.11987717935160659No Hit
TTCAAGTAATCCAGGATAGGCTT112700.11813709437675816No Hit
TGTAAACATCCCCGACTGGAAGC109660.11495043273607188No Hit
TACGCCTGTCTGAGCGTCGCT107760.11295876921064295No Hit
TGAGGTAGTAGGTTGTATAGT106310.11143881546755245No Hit
AACATTCATTGCTGTCGGTGGGT105400.11048491346326807No Hit
ACTGGACTTGGAGTCAGAAGG104160.10918509095193549No Hit
CCCCCCACTGCTAAATTTTACTG103550.10854566213587674No Hit
TGAATACCGGGTGCTGTAGGCTT101690.10659592836887788No Hit
TATTGCACTTGTCCCGGCCTGTAAT98420.10316817061721863No Hit
TATTGCACTTGTCCCGGCCTGTAAA96770.10143856808197771No Hit
GTCAAGTAATCCAGGATAGGCT96280.1009249285412092No Hit
TGAGGGGCAGAGAGCGAGACTTT96270.10091444610160168No Hit
ACTGGACTTGGAGTCAGAAGGCA95850.1004741836380858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)