FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8263794
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG88538310.714001341272544No Hit
CCCCCCACTGCTAAATTTGACTG7359458.905655199052639No Hit
AACATTCAACGCTGTCGGTGAGT3539364.282972203808566No Hit
TTCAAGTAATCCAGGATAGGCT2732573.30667729616687No Hit
AACATTCAACGCTGTCGGTGAG2628463.180694000842712No Hit
TAGCAGCACGTAAATATTGGCG2552173.088375629886224No Hit
AAGCTGCCAGTTGAAGAACTGT2510923.038459090340345No Hit
CCCCCACTGCTAAATTTGACTG2082302.5197869162759865No Hit
TATTGCACTTGTCCCGGCCTGTAA1771032.1431197341076023No Hit
TAGCTTATCAGACTGATGTTGACT1594211.929150218410575No Hit
TATTGCACTTGTCCCGGCCTGTAT1353591.637976454882588No Hit
TAGCTTATCAGACTGATGTTGAC1321051.5985998682929417No Hit
TAGCTTATCAGACTGATGTTGA1220281.4766583000495899No Hit
TGAGGTAGTAGGTTGTATAGTT910951.1023387078622726No Hit
TGTAAACATCCTTGACTGGAAGCT867981.050340799879571No Hit
TATTGCACTTGTCCCGGCCTGT715340.8656314520908919No Hit
TATTGCACTTGTCCCGGCCTGTA663610.8030330862555384No Hit
AGCAGCATTGTACAGGGCTATGA620880.7513256017756492No Hit
CATAAAGTAGAAAGCACTACT609170.7371553550342614No Hit
TGTAAACATCCCCGACTGGAAGCT593930.7187134626056748No Hit
TGAGGTAGTAGATTGTATAGTT507350.6139431839661057No Hit
CAACGGAATCCCAAAAGCAGCTGT480580.5815488624232404No Hit
CCCATAAAGTAGAAAGCACTAC458610.554963010936623No Hit
CAACGGAATCCCAAAAGCAGCT415960.5033523342910048No Hit
TTCAAGTAATTCAGGATAGGTT401740.48614474174937083No Hit
ACTGGACTTGGAGTCAGAAGGC379320.45901434619498016No Hit
CCCCCACTGCTAAATTTGACTGGCTT366010.44290794276817647No Hit
CCCATAAAGTAGAAAGCACT365260.4420003693218878No Hit
TCGTACGACTCTTAGCGG350770.4244660503395898No Hit
CAAAGAATTCTCCTTTTGGGCT295640.35775335154772736No Hit
AACATTCAACGCTGTCGGTGAGTT286340.3464994408137473No Hit
CATTGCACTTGTCTCGGTCTGA260520.31525471230284785No Hit
TGTAAACATCCTACACTCTCAGCT257080.3110919754292036No Hit
TTCTCACTACTGCACTTGACTAG244450.29580843859370165No Hit
TGAGGTAGTAGTTTGTACAGTT231020.2795568234154917No Hit
CACCCGGCTGTGTGCACATGTGC228660.27670099230450323No Hit
TCGTTTCCCGGCCAATGCACCA222580.2693435968999227No Hit
TAGCTTATCAGACTGATGTTGACA215590.2608850123805119No Hit
TGTCAGTTTGTCAAATACCCCAA214630.2597233183692624No Hit
ACCCCCCACTGCTAAATTTGACTG207160.25068388684422677No Hit
CTCGTACGACTCTTAGCGG200250.24232211015908672No Hit
CAACACCAGTCGATGGGCTGT195170.23617481268289117No Hit
CTTCTCACTACTGCACTTGACTAG195170.23617481268289117No Hit
AACGGAATCCCAAAAGCAGCTG187820.22728059290926175No Hit
TATTGCACTTGTCCCGGCCTGTAG185770.22479989215607263No Hit
CCCCCCACTGCTAAATTTGACTGGCT170380.20617648503822822No Hit
TAGCAGCACGTAAATATTGGC161460.19538241151703445No Hit
TATTGCACTTGTCCCGGCCTGTT161120.19497097822138354No Hit
TTCACAGTGGCTAAGTTCCG156190.18900519543444572No Hit
TATTGCACTTGTCCCGGCCTGTAAA150530.1821560411597869No Hit
TGAGGTAGTAGTTTGTGCTGTT146410.1771704376948409No Hit
TGTAAACATCCCCGACTGGAAGC144330.1746534340038002No Hit
TTCAAGTAATCCAGGATAGGC141090.17073271671583296No Hit
CCCCCCACTGCTAAATTTGACTGG140480.16999455697951812No Hit
AGCAGCATTGTACAGGGCTATGT140230.1696920324974219No Hit
CCCATAAAGTAGAAAGCACTA138250.16729603859921968No Hit
CAAAGAATTCTCCTTTTGGGCTT137630.16654577788362102No Hit
TATTGCACTTGTCCCGGCCTGTAAT134820.1631454027048593No Hit
CTCCCACTGCTTCACTTGACTAG134240.16244354590639604No Hit
ACTGGACTTGGAGTCAGAAGGCT132510.16035007649029004No Hit
ACTGGACTTGGAGTCAGAAGGCA127910.15478362601971926No Hit
ATGCACCTGGGCAAGGATTCTGA127600.15440849566191991No Hit
TGTCAGTTTGTCAAATACCCCA125690.15209720861870468No Hit
TTCAAGTAATCCAGGATAGGCTT125460.15181888609517613No Hit
AACATTCAACGCTGTCGGTGA120570.14590150722537373No Hit
TCGTTTCCCGGGCGGCGCACCA118500.14339660451361688No Hit
CAACGGAATCCCAAAAGCAGCTGA117750.14248903106732816No Hit
TATTGCACTTGTCCCGGCCTGTTT116210.14062548025761534No Hit
TTCACAGTGGCTAAGTTCTG112210.1357850885440755No Hit
AACATTCAACGCTGTCGGTGAGA111870.13537365524842465No Hit
AGCTCGGTCTGAGGCCCCTCAGT111540.1349743229320576No Hit
CCCCCCACTGCTAAATTTGACTGTA107940.13061797038987177No Hit
GGAATACCGGGTGCTGTAGGCTTT107890.13055746549345254No Hit
CGCGACCTCAGATCAGACGTGGCG104990.12704818150113614No Hit
CGCCTGTCTGAGCGTCGCT100980.1221956888083125No Hit
CACTAGATTGTGAGCTCCTGGAA100290.12136072123772687No Hit
CACTAGATTGTGAGCTCCTGGA95620.11570956391216915No Hit
TTATAAAGCAATGAGACTGATT93240.11282953084261298No Hit
TAGCAGCACATCATGGTTTACA91830.11112329276359019No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC91790.11107488884645479No Hit
TATTGCACTTGTCCCGGCCTGA91070.11020361833801763No Hit
ACTGGACTTGGAGTCAGAAGG89350.1081222499011955No Hit
AGCAGCATTGTACAGGGCTATG89130.10785602835695081No Hit
ATCACATTGCCAGGGATTTCC88120.10663382944928201No Hit
TTCACAGTGGCTAAGTTCTGC86810.10504860116309772No Hit
GGAATACCGGGTGCTGTAGGCTT85550.10352387777333269No Hit
CAAAGTGCTGTTCGTGCAGGTAG85110.1029914346848433No Hit
CCCCCACTGCTAAATTTGACTGGCTA84180.1018660436114453No Hit
TGAGGTAGTAGGTTGTGTGGTT83150.1006196427452088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)