FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7220169
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG77807210.776368253984083No Hit
CAACGGAATCCCAAAAGCAGCTG4341816.013446499659496No Hit
TAGCTTATCAGACTGATGTTGAC2672223.70104910286726No Hit
AACATTCAACGCTGTCGGTGAGT2292633.1753134864294728No Hit
TAGCAGCACGTAAATATTGGCG2219143.0735291653145516No Hit
AAGCTGCCAGTTGAAGAACTGT2092182.897688405908504No Hit
TAGCTTATCAGACTGATGTTGACT2054292.8452104098948374No Hit
CCCCCACTGCTAAATTTGACTG2005892.7781759679032443No Hit
TTCAAGTAATCCAGGATAGGCT1775192.4586543611375298No Hit
AACATTCAACGCTGTCGGTGAG1507662.0881228680381305No Hit
TATTGCACTTGTCCCGGCCTGTAA1216121.6843373056780249No Hit
TATTGCACTTGTCCCGGCCTGTAT1124511.557456619090218No Hit
TGTAAACATCCTTGACTGGAAGCT1075451.4895080710714665No Hit
TAGCTTATCAGACTGATGTTGA935011.2949973885652815No Hit
TGAGGTAGTAGGTTGTATAGTT773631.071484614833808No Hit
AGCAGCATTGTACAGGGCTATGA680880.9430250178354551No Hit
TGAGGTAGTAGATTGTATAGTT590320.8175985908363086No Hit
TGTAAACATCCCCGACTGGAAGCT499300.6915350596364157No Hit
ACTGGACTTGGAGTCAGAAGGC484260.6707045222902677No Hit
TATTGCACTTGTCCCGGCCTGT483400.6695134144366981No Hit
TATTGCACTTGTCCCGGCCTGTA483370.6694718641627364No Hit
TTCAAGTAATTCAGGATAGGTT399720.5536158502661087No Hit
TAGCTTATCAGACTGATGTTGACA357460.4950853643453498No Hit
CGCCTGTCTGAGCGTCGCT349710.4843515435719026No Hit
CATAAAGTAGAAAGCACTACT347890.4818308269515575No Hit
TGTAAACATCCTACACTCTCAGCT311670.43166579618842715No Hit
CCCCCACTGCTAAATTTGACTGGCTT309970.4293112806639291No Hit
TGTCAGTTTGTCAAATACCCCAA284280.39373039606136645No Hit
CAACGGAATCCCAAAAGCAGCTGT273310.3785368458826933No Hit
TGAGGTAGTAGTTTGTACAGTT230590.31936925576118785No Hit
CAAAGAATTCTCCTTTTGGGCT225960.31295666347976064No Hit
CATTGCACTTGTCTCGGTCTGA222970.3088154861749081No Hit
CCCATAAAGTAGAAAGCACTAC215840.2989403710633366No Hit
AACATTCAACGCTGTCGGTGAGTT212480.2942867403796227No Hit
TGTCAGTTTGTCAAATACCCCA199060.2756999178274082No Hit
GGAATACCGGGTGCTGTAGGCTTT198150.2744395595172357No Hit
CTTCTCACTACTGCACTTGACTAG189450.2623899800683336No Hit
CCCCCCACTGCTAAATTTGACTGG188480.26104652121023764No Hit
CCCCCCACTGCTAAATTTGACTGGCT181840.2518500605733744No Hit
CAACGGAATCCCAAAAGCAGCT180020.24932934395302933No Hit
ACTGGACTTGGAGTCAGAAGGCA179460.24855373883907705No Hit
TTCTCACTACTGCACTTGACTAG176390.24430176080365984No Hit
ACCCCCCACTGCTAAATTTGACTG171410.23740441532601247No Hit
CAACACCAGTCGATGGGCTGT165200.22880350861593404No Hit
CACTAGATTGTGAGCTCCTGGAA162910.22563183770352188No Hit
TAGCAGCACGTAAATATTGGC156920.21733563300249623No Hit
CAAAGAATTCTCCTTTTGGGCTT154830.21444096391649556No Hit
TGAGGTAGTAGTTTGTGCTGTT154710.21427476282064864No Hit
TATTGCACTTGTCCCGGCCTGTAG147600.20442734789171835No Hit
TTATAAAGCAATGAGACTGATT145810.20194818154533503No Hit
CACTAGATTGTGAGCTCCTGGA142450.1972945508616211No Hit
AGCAGCATTGTACAGGGCTATGT138150.19133901159377295No Hit
TACGCCTGTCTGAGCGTCGCT136620.18921994762172464No Hit
CTCCCACTGCTTCACTTGACTAG134080.18570202442629805No Hit
TATTGCACTTGTCCCGGCCTGTAAT131970.1827796551576563No Hit
TGTAAACATCCTACACTCAGCT129740.1796910847931676No Hit
CACCCGGCTGTGTGCACATGTGC127830.17704571735093735No Hit
TGTAAACATCCCCGACTGGAAGC126550.17527290566190348No Hit
GGAATACCGGGTGCTGTAGGCTT124170.17197658392760612No Hit
CCCCCCACTGCTAAATTTGACTGTA123280.17074392580007475No Hit
TTCAAGTAATCCAGGATAGGCTT123010.17036997333441917No Hit
ACTGGACTTGGAGTCAGAAGGCT119660.16573019274202583No Hit
CCCCCCACTGCTAAATTTGACTGT119250.16516233899788219No Hit
ATCACATTGCCAGGGATTTCCA115960.1606056589534123No Hit
CCCATAAAGTAGAAAGCACT115840.1604394578575654No Hit
GCCTGTCTGAGCGTCGCT113320.15694923484477996No Hit
AACGGAATCCCAAAAGCAGCTG110750.15338976137539165No Hit
GCGTTCGCGCTTTCCCCTG109270.1513399478599462No Hit
TATTGCACTTGTCCCGGCCTGTAAA108560.15035659137618523No Hit
TATTGCACTTGTCCCGGCCTGTT107850.14937323489242427No Hit
CTCCCACTGCTTCACTTGACTAGCCT107650.14909623306601272No Hit
TAGCAGCACGTAAATATTGGCGT104800.14514895703964825No Hit
CTACGCCTGTCTGAGCGTCGCT104510.1447473043913515No Hit
AGCTCGGTCTGAGGCCCCTCAGT102450.14189418557931263No Hit
ACTGGACTTGGAGTCAGAAGG98620.1365896006035316No Hit
TGTGCAAATCCATGCAAAACTG95080.13168666827604728No Hit
TTCACAGTGGCTAAGTTCCGC93410.1293737030255109No Hit
TATTGCACTTGTCCCGGCCTGTTT92170.12765629170175932No Hit
ATCACATTGCCAGGGATTTCC91360.12653443430479258No Hit
ATGCACCTGGGCAAGGATTCTGA91210.12632668293498392No Hit
CAGTGCAATAGTATTGTCAAAGC90170.12488627343764391No Hit
CGCGACCTCAGATCAGACGTGGCG90000.12465082188519411No Hit
TCGTTTCCCGGCCAATGCACCA89120.12343201384898332No Hit
CAAAGTGCTGTTCGTGCAGGTAG88290.12228245626937542No Hit
TAGCTTATCAGACTGATGTTGACC86540.11985869028827442No Hit
TTCACAGTGGCTAAGTTCCG85540.11847368115621672No Hit
AGCAGCATTGTACAGGGCTATG82970.11491420768682838No Hit
TGTAAACATCCTTGACTGGAAGC82700.1145402552211728No Hit
ACCACAGGGTAGAACCACGGAC82130.1137508000158999No Hit
TCGTACGACTCTTAGCGG77570.10743515837371674No Hit
TGTCAGTTTGTCAAATACCCC76680.10620250024618537No Hit
TAGCAGCACATCATGGTTTACA75910.10513604321450093No Hit
GGGCTACGCCTGTCTGAGCGTCGCT73520.101825871388883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)