FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12420154
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8754817.048873951160347No Hit
CCCCCCACTGCTAAATTTGACTG6855995.520052327853583No Hit
TTCAAGTAATCCAGGATAGGCT5264244.238465964270652No Hit
TAGCAGCACGTAAATATTGGCG4406283.5476854795842305No Hit
AAGCTGCCAGTTGAAGAACTGT4275773.4426062672008735No Hit
AACATTCAACGCTGTCGGTGAGT3289722.648695016180959No Hit
TAGCTTATCAGACTGATGTTGAC3259362.624250874828122No Hit
TAGCTTATCAGACTGATGTTGA3006942.421016679825387No Hit
TAGCTTATCAGACTGATGTTGACT2727792.196261012544611No Hit
CATAAAGTAGAAAGCACTACT2642262.1273971321128546No Hit
AACATTCAACGCTGTCGGTGAG2293501.8465954608936412No Hit
CCCCCACTGCTAAATTTGACTG2268471.8264427317084795No Hit
TGAGGTAGTAGATTGTATAGTT1644001.3236550851140816No Hit
TGAGGTAGTAGGTTGTATAGTT1561771.257448176568503No Hit
TTCAAGTAATTCAGGATAGGTT1534801.2357334699714673No Hit
TATTGCACTTGTCCCGGCCTGTAT1484691.1953877544513538No Hit
TATTGCACTTGTCCCGGCCTGTAA1413671.1382064988888223No Hit
TGTAAACATCCTTGACTGGAAGCT1307341.0525956441441868No Hit
TATTGCACTTGTCCCGGCCTGT1057680.8515836438098915No Hit
AGCAGCATTGTACAGGGCTATGA762940.6142757972244144No Hit
TATTGCACTTGTCCCGGCCTGTA712650.5737851559650549No Hit
GGAATACCGGGTGCTGTAGGCTTT690700.5561122672069927No Hit
CATTGCACTTGTCTCGGTCTGA635640.5117810938576124No Hit
TCGTACGACTCTTAGCGG613360.4938425079109325No Hit
CCCATAAAGTAGAAAGCACTAC598370.4817734144037184No Hit
TGAGGTAGTAGTTTGTACAGTT595640.479575374025153No Hit
TGTAAACATCCCCGACTGGAAGCT542430.43673371521802384No Hit
CAACGGAATCCCAAAAGCAGCTGT523700.4216533869064748No Hit
ACTGGACTTGGAGTCAGAAGGC517510.4166695517624016No Hit
TTCTCACTACTGCACTTGACTAG489450.3940772392999314No Hit
TGTAAACATCCTACACTCTCAGCT457940.36870718350191145No Hit
CAACACCAGTCGATGGGCTGT444800.3581276045369486No Hit
CAAAGAATTCTCCTTTTGGGCT439070.35351413517094876No Hit
GGAATACCGGGTGCTGTAGGCTT425670.34272521902707487No Hit
TGAGGTAGTAGTTTGTGCTGTT392410.3159461629863849No Hit
TGTCAGTTTGTCAAATACCCCAA384600.3096579961890972No Hit
CAACGGAATCCCAAAAGCAGCT382990.3083617159658407No Hit
TTCACAGTGGCTAAGTTCCGC368410.29662273108690923No Hit
TGTCAGTTTGTCAAATACCCCA361680.29120411872509794No Hit
TTCACAGTGGCTAAGTTCCG359010.28905438692628127No Hit
TTATAAAGCAATGAGACTGATT351270.2828225801387004No Hit
CTTCTCACTACTGCACTTGACTAG339240.2731367098990882No Hit
GCCTGTCTGAGCGTCGCT334400.2692398177993606No Hit
CTCCCACTGCTTCACTTGACTAG325390.26198547940709915No Hit
TGAGAACTGAATTCCATGGGTT317890.255946906938513No Hit
TTAATGCTAATCGTGATAGGGGTT304060.24481177930644016No Hit
TAGCTTATCAGACTGATGTTGACA303810.2446104935574873No Hit
CCCCCACTGCTAAATTTGACTGGCTT301120.24244465889875438No Hit
CAAAGAATTCTCCTTTTGGGCTT292940.23585858919301644No Hit
TTCAAGTAATCCAGGATAGGCTT289250.23288761153847207No Hit
AACCGGGCGGAAACACCA283840.22853178793113194No Hit
ATCCCGGACGAGCCCCCA282430.22739653630703774No Hit
CCCATAAAGTAGAAAGCACT278340.22410350145416874No Hit
CACCCGGCTGTGTGCACATGTGC276940.22297630126003268No Hit
CACTAGATTGTGAGCTCCTGGA256590.20659164129526894No Hit
TAGCAGCACGTAAATATTGGC253330.20396687512892353No Hit
AACATTCAACGCTGTCGGTGAGTT249270.2006979945659289No Hit
TTCACAGTGGCTAAGTTCTGC242650.19536794793365686No Hit
AGCTCGGTCTGAGGCCCCTCAGT236550.19045657565920682No Hit
CACTAGATTGTGAGCTCCTGGAA229080.184442157480495No Hit
TTCAAGTAATCCAGGATAGGC227690.18332300871631704No Hit
CGCCTGTCTGAGCGTCGCT226060.18201062563314432No Hit
CTACGCCTGTCTGAGCGTCGCT214950.17306548694967872No Hit
TATTGCACTTGTCCCGGCCTGTT201460.16220410793618179No Hit
TGTAAACATCCTACACTCAGCT199100.1603039704660667No Hit
TTCACAGTGGCTAAGTTCTG195960.15777582145921865No Hit
AACGGAATCCCAAAAGCAGCTG195210.15717196421236002No Hit
TGTCAGTTTGTCAAATACCCC188840.15204320332904087No Hit
TGTCAGTTTGTCAAATACC185360.14924130570361688No Hit
CCCCCCACTGCTAAATTTGACTGG184830.1488145799158368No Hit
ACCGGGTGCTGTAGGCTTA184570.14860524273692582No Hit
AGCAGCATTGTACAGGGCTATGT184410.14847641985759596No Hit
TGTAAACATCCCCGACTGGAAGC180370.14522364215451758No Hit
CCCCCCACTGCTAAATTTGACTGGCT178390.14362945902281082No Hit
TGAATACCGGGTGCTGTAGGCTTT177020.1425264131185491No Hit
TGATGTAGTAGATTGTATAGTT175930.14164880725311457No Hit
TCGTTTCCCGGCCAATGCACCA168960.13603696057230852No Hit
TGATGTAGTAGGTTGTATAGTT168260.1354733604752405No Hit
TATTGCACTTGTCCCGGCCTGTAG166920.1343944688608531No Hit
ATACCGGGTGCTGTAGGCTTA166810.13430590313131383No Hit
ATCACATTGCCAGGGATTTCC163680.13178580555442387No Hit
TGAGGTAGTAGATTGTATAGT163270.13145569692614117No Hit
TCAGTGCACTACAGAACTTTGT156590.12607734171412047No Hit
CCCATAAAGTAGAAAGCACTA153750.12379073560601582No Hit
ACCGGGTGCTGTAGGCTT152880.12309026119965985No Hit
CTCCCACTGCTTCACTTGACTAGCCT152630.12288897545070698No Hit
TAGCAGCACGTAAATATTGGCGT150780.12139946090845573No Hit
CAGTGCAATAGTATTGTCAAAGC150500.1211740208696285No Hit
TGAGGTAGTAGGTTGTATAGT148650.11968450632737727No Hit
TGTCAGTTTGTCAAATACCC147840.11903234050076995No Hit
TCGTTTCCCGGGCGGCGCACCA145250.1169470201416182No Hit
TAGCTTATCAGACTGATGTTG144830.11660886008337738No Hit
ACCGGGTGCTGTAGGCTTT144320.11619823715551354No Hit
TTCACAGTGGCTAAGTTCCGCT142000.11433030540523088No Hit
TATTGCACTTGTCCCGGCCTGTTT140600.1132031052110948No Hit
TCAGTGCATCACAGAACTTTGTTT138760.11172164209880167No Hit
TAGCAGCACATCATGGTTTACA137880.11101311626248757No Hit
ACTGGACTTGGAGTCAGAAGG137330.11057028761479125No Hit
TGAGGTAGTAAGTTGTATTGTT137210.11047367045529388No Hit
TACGCCTGTCTGAGCGTCGCT136220.1096765788894405No Hit
ACTGGACTTGGAGTCAGAAGGCT134780.10851717297547195No Hit
CAAAGTGCTGTTCGTGCAGGT132140.10639159546652964No Hit
ACTGGACTTGGAGTCAGAAGGCA131550.10591656109900087No Hit
ATACCGGGTGCTGTAGGCTTT130390.10498259522385954No Hit
ATCACATTGCCAGGGATTTCCA129300.10410498935842502No Hit
TAGCAGCACATAATGGTTTGT128580.10352528640144075No Hit
GGAATACCGGGTGCTGTAGGCTTA127090.10232562333768165No Hit
TGTAGTGTTTCCTACTTTATGGA126590.1019230518397759No Hit
TTCAAGTAATTCAGGATAGGTTA125290.10087636594522097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)