FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11676729
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7158056.130184232245178No Hit
AAGCTGCCAGTTGAAGAACTGT6677365.718519287379197No Hit
TAGCAGCACGTAAATATTGGCG4913174.20765952519751No Hit
TTCAAGTAATCCAGGATAGGCT4600263.9396820804867527No Hit
AACATTCAACGCTGTCGGTGAGT4404643.7721522868262163No Hit
CCCCCCACTGCTAAATTTGACTG3915513.353259290337217No Hit
TAGCTTATCAGACTGATGTTGAC3730493.194807381416491No Hit
AACATTCAACGCTGTCGGTGAG2322871.9893156722229317No Hit
TAGCTTATCAGACTGATGTTGACT2286591.9582453270945999No Hit
TAGCTTATCAGACTGATGTTGA2284031.9560529322895137No Hit
CATAAAGTAGAAAGCACTACT1762041.5090184931071022No Hit
TGTAAACATCCTTGACTGGAAGCT1731701.4830351890499471No Hit
TGAGGTAGTAGGTTGTATAGTT1469991.2589056404409147No Hit
CCCCCACTGCTAAATTTGACTG1441481.2344895561077078No Hit
TATTGCACTTGTCCCGGCCTGT1151210.9859011029544319No Hit
TGAGGTAGTAGATTGTATAGTT1126810.9650048399684535No Hit
TTCAAGTAATTCAGGATAGGTT1110750.9512509881834201No Hit
TATTGCACTTGTCCCGGCCTGTAA1051010.9000894000366028No Hit
TATTGCACTTGTCCCGGCCTGTAT966760.8279373444395258No Hit
AGCAGCATTGTACAGGGCTATGA951040.814474670089543No Hit
TGTCAGTTTGTCAAATACCCCAA763640.6539845191234633No Hit
TGTAAACATCCCCGACTGGAAGCT684480.5861915610099369No Hit
CCCATAAAGTAGAAAGCACTAC624030.5344219258663964No Hit
TGTAAACATCCTACACTCTCAGCT619960.5309363606879974No Hit
CGCCTGTCTGAGCGTCGCT602860.5162918485133979No Hit
TATTGCACTTGTCCCGGCCTGTA593640.5083958015982044No Hit
TTAATGCTAATCGTGATAGGGGTT584230.500337037881071No Hit
TGTCAGTTTGTCAAATACCCCA551490.4722983636941475No Hit
TGAGGTAGTAGTTTGTACAGTT537860.46062557416550476No Hit
CATTGCACTTGTCTCGGTCTGA503070.4308312713260708No Hit
ACTGGACTTGGAGTCAGAAGGC475160.4069290295253063No Hit
CAACGGAATCCCAAAAGCAGCTGT467050.39998359129513067No Hit
TTCTCACTACTGCACTTGACTAG460460.394339887480475No Hit
TCGTACGACTCTTAGCGG451870.3869833752243458No Hit
CCCATAAAGTAGAAAGCACT435000.37253583602051565No Hit
TGTAAACATCCTACACTCAGCT375900.32192234657496976No Hit
AACATTCAACGCTGTCGGTGAGTT360710.3089135664619775No Hit
TAGCTTATCAGACTGATGTTGACA345020.29547658423861684No Hit
TGATGTAGTAGGTTGTATAGTT344930.2953995078587505No Hit
TGAGGTAGTAGTTTGTGCTGTT321260.27512841995391No Hit
CTACGCCTGTCTGAGCGTCGCT319430.27356120022996167No Hit
TTATAAAGCAATGAGACTGATT300710.25752931321776845No Hit
CAACACCAGTCGATGGGCTGT300060.2569726504742895No Hit
TAGCAGCACGTAAATATTGGC289040.24753507596176977No Hit
TGAGAACTGAATTCCATGGGTT285420.24443489268270252No Hit
TTCACAGTGGCTAAGTTCCG283300.24261931573474044No Hit
AACCGGGCGGAAACACCA283220.24255080339708152No Hit
TTCACAGTGGCTAAGTTCCGC281050.2406924062380826No Hit
TGATGTAGTAGATTGTATAGTT266090.22788059909585984No Hit
CAAAGAATTCTCCTTTTGGGCT263040.2252685662226125No Hit
TTCAAGTAATCCAGGATAGGCTT254410.21787779779765376No Hit
TACGCCTGTCTGAGCGTCGCT250240.21430659219718126No Hit
TGTAAACATCCCCGACTGGAAGC242090.2073268977981762No Hit
CTTCTCACTACTGCACTTGACTAG239540.2051430670352973No Hit
TAGCAGCACGTAAATATTGGCGT236390.2024453937399763No Hit
TTCAAGTAATCCAGGATAGGC222350.1904214784808314No Hit
CAAAGAATTCTCCTTTTGGGCTT221400.1896078944711314No Hit
TGTCAGTTTGTCAAATACCCC215330.18440952085125895No Hit
TTCACAGTGGCTAAGTTCTGC212900.1823284585948685No Hit
ACGCCTGTCTGAGCGTCGCT186840.16001056460246701No Hit
AGCTCGGTCTGAGGCCCCTCAGT185570.15892293124213125No Hit
CAACGGAATCCCAAAAGCAGCT182960.15668771622600816No Hit
TATTGCACTTGTCCCGGCCTGTT180520.15459808992741034No Hit
ATCACATTGCCAGGGATTTCC171890.14720732150245158No Hit
GCCTGTCTGAGCGTCGCT171890.14720732150245158No Hit
AGCAGCATTGTACAGGGCTATGT171640.14699322044726737No Hit
CCCCCACTGCTAAATTTGACTGGCTT171460.14683906768753477No Hit
TAGCAGCACATAATGGTTTGT170230.14578569049602846No Hit
GCGTTCGCGCTTTCCCCTG165940.14211171638906753No Hit
CTCCCACTGCTTCACTTGACTAG164700.14104977515535388No Hit
TAGCAGCACATCATGGTTTACA163020.139611016064516No Hit
TGTAAACATCCTTGACTGGAAGC161760.13853194674638764No Hit
AACGGAATCCCAAAAGCAGCTG160470.13742718530163714No Hit
AGCAGCATTGTACAGGGCTATG156710.1342071054316667No Hit
AACATTCAACGCTGTCGGTGA154200.1320575308376173No Hit
TGAGAACTGAATTCCATAGGCTGT152410.13052456728249837No Hit
CACCCGGCTGTGTGCACATGTGC151740.1299507764546047No Hit
ACCGGGTGCTGTAGGCTT150650.1290172958540016No Hit
CAAAGTGCTGTTCGTGCAGGT148930.12754428059433426No Hit
ACCACAGGGTAGAACCACGGAC147290.12613977767232587No Hit
GGGCTACGCCTGTCTGAGCGTCGCT146530.1254889104645659No Hit
TGAGGTAGTAGGTTGTATAGT146280.12527480940938168No Hit
TCGTTTCCCGGCCAATGCACCA145800.12486373538342802No Hit
ATCACATTGCCAGGGATTTCCA143320.1227398529160007No Hit
CAAAGTGCTGTTCGTGCAGGTAG137540.1177898365201419No Hit
GGAATACCGGGTGCTGTAGGCTTT135180.115768722559203No Hit
TGAGGTAGTAGGTTGTGTGGTT132300.11330227840348098No Hit
CCCCCCACTGCTAAATTTGACTGG131970.11301966501063782No Hit
ATCCCGGACGAGCCCCCA130250.11154664975097049No Hit
GTAAACATCCTTGACTGGAAGCT129090.1105532208549158No Hit
ATCCCACCGCTGCCACCA128920.11040763213739054No Hit
ACCGGGTGCTGTAGGCTTT128570.11010789066013264No Hit
TAGCACCATCTGAAATCGGTTA126060.10795831606608323No Hit
TGATGTAGTAGTTTGTACAGTT125080.10711903992976116No Hit
TTCACAGTGGCTAAGTTCTG124480.10660519739731907No Hit
TAGCTTATCAGACTGATGTTG123400.10568028083892331No Hit
ACTGGACTTGGAGTCAGAAGG123370.10565458871230118No Hit
AGAGGTAGTAGGTTGCATAGTT121000.10362491070915493No Hit
CCCATAAAGTAGAAAGCACTA120710.10337655348514126No Hit
CACTAGATTGTGAGCTCCTGGA120260.10299117158580968No Hit
TATTGCACTTGTCCCGGCCTGTAG120250.10298260754360233No Hit
TTCACCACCTTCTCCACCCAGC119880.1026657379819297No Hit
TGTCAGTTTGTCAAATACCC119790.1025886616020634No Hit
ACTGGACTTGGAGTCAGAAGGCA118730.10168087312808236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)