FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9455366
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5558805.878989771522329No Hit
AAGCTGCCAGTTGAAGAACTGT4742095.015236850694093No Hit
TTCAAGTAATCCAGGATAGGCT3774253.9916487632525275No Hit
TAGCAGCACGTAAATATTGGCG3691723.904364992322878No Hit
AACATTCAACGCTGTCGGTGAGT3375773.5702161079750905No Hit
CCCCCCACTGCTAAATTTGACTG3177563.3605891088721473No Hit
TAGCTTATCAGACTGATGTTGAC2378922.515947029443387No Hit
AACATTCAACGCTGTCGGTGAG2016392.13253511286607No Hit
TAGCTTATCAGACTGATGTTGA1801281.9050346649722498No Hit
TAGCTTATCAGACTGATGTTGACT1596871.6888505426442508No Hit
CATAAAGTAGAAAGCACTACT1462301.5465292406449418No Hit
CCCCCACTGCTAAATTTGACTG1263181.3359398250686434No Hit
TGTAAACATCCTTGACTGGAAGCT1160961.2278318998968416No Hit
TGAGGTAGTAGGTTGTATAGTT1141031.2067539215298488No Hit
TATTGCACTTGTCCCGGCCTGT872430.9226824218121223No Hit
GCCTGTCTGAGCGTCGCT856980.9063424937754921No Hit
TGAGGTAGTAGATTGTATAGTT838290.8865759400535104No Hit
TATTGCACTTGTCCCGGCCTGTAA813890.8607704873613564No Hit
TTCAAGTAATTCAGGATAGGTT790940.8364985554234495No Hit
TATTGCACTTGTCCCGGCCTGTAT755910.799450809201886No Hit
AACCGGGCGGAAACACCA716650.7579294127800024No Hit
CGCCTGTCTGAGCGTCGCT691490.7313201836925192No Hit
AGCAGCATTGTACAGGGCTATGA691080.7308865674792494No Hit
TTAATGCTAATCGTGATAGGGGTT546760.5782536604082803No Hit
TATTGCACTTGTCCCGGCCTGTA545260.576667259628025No Hit
CCCATAAAGTAGAAAGCACTAC436970.4621396992987897No Hit
TGTAAACATCCTACACTCTCAGCT430290.4550749278240525No Hit
TGTAAACATCCCCGACTGGAAGCT418950.443081737925322No Hit
CATTGCACTTGTCTCGGTCTGA414160.43801583143370654No Hit
TTCACAGTGGCTAAGTTCCG413420.43723320704878055No Hit
TGAGGTAGTAGTTTGTACAGTT402770.42596976150896754No Hit
CCCATAAAGTAGAAAGCACT390990.4135112273813621No Hit
CAACACCAGTCGATGGGCTGT354310.3747184403015177No Hit
ACTGGACTTGGAGTCAGAAGGC352220.37250805521436187No Hit
TGTCAGTTTGTCAAATACCCCAA338370.3578602880100041No Hit
TTCTCACTACTGCACTTGACTAG337970.357437247801936No Hit
TGTCAGTTTGTCAAATACCCCA320750.3392253668446044No Hit
CAACGGAATCCCAAAAGCAGCTGT319640.33805143026721546No Hit
AACATTCAACGCTGTCGGTGAGTT282480.29875099493768936No Hit
TTATAAAGCAATGAGACTGATT258820.27372816663046146No Hit
CTACGCCTGTCTGAGCGTCGCT254590.26925451643014137No Hit
TGTAAACATCCTACACTCAGCT250630.26506641837026723No Hit
TGAGGTAGTAGTTTGTGCTGTT235960.2495514187393698No Hit
TACGCCTGTCTGAGCGTCGCT229960.24320581561834836No Hit
TAGCTTATCAGACTGATGTTGACA225460.2384466132775823No Hit
ACCGGGTGCTGTAGGCTT222250.2350517156078358No Hit
TAGCAGCACGTAAATATTGGC217760.23030308927227142No Hit
TCGTACGACTCTTAGCGG217260.2297742890121863No Hit
TGATGTAGTAGGTTGTATAGTT202430.21409007329806165No Hit
TGAGAACTGAATTCCATGGGTT198700.21014522335782668No Hit
CAAAGAATTCTCCTTTTGGGCT197340.2087068866503951No Hit
ACGCCTGTCTGAGCGTCGCT194930.20615806939678483No Hit
ATCCCGGACGAGCCCCCA191160.20217091543574306No Hit
TGTCAGTTTGTCAAATACC186770.1975280491521957No Hit
CTTCTCACTACTGCACTTGACTAG185620.19631180855399993No Hit
TAGCAGCACGTAAATATTGGCGT184750.1953916961014518No Hit
ACCGGGTGCTGTAGGCTTT179500.18983929337055805No Hit
TTCAAGTAATCCAGGATAGGC175930.18606365951355028No Hit
TTCAAGTAATCCAGGATAGGCTT166640.17623855068116875No Hit
TTCACAGTGGCTAAGTTCTG151430.16015244676937943No Hit
TGATGTAGTAGATTGTATAGTT150770.15945443042606705No Hit
AGCTCGGTCTGAGGCCCCTCAGT149480.15809012575504744No Hit
TTCACAGTGGCTAAGTTCCGC149030.15761420552097083No Hit
TATTGCACTTGTCCCGGCCTGTT148940.15751902147415553No Hit
ACCGGGTGCTGTAGGCTTA148900.1574767174533487No Hit
AGCAGCATTGTACAGGGCTATGT147950.15647199695918698No Hit
GCGTTCGCGCTTTCCCCTG145330.15370108359634094No Hit
TTCACAGTGGCTAAGTTCTGC142830.15105708229591536No Hit
AGCAGCATTGTACAGGGCTATG140980.1491005213336004No Hit
AACGGAATCCCAAAAGCAGCTG140700.14880439318795274No Hit
CTCCCACTGCTTCACTTGACTAG136370.14422498293561561No Hit
CAACGGAATCCCAAAAGCAGCT136120.14396058280557303No Hit
AGCTACATTGTCTGCTGGGTTT133830.14153867761438318No Hit
TAGCAGCACATAATGGTTTGT133410.14109448539591168No Hit
CCCCCACTGCTAAATTTGACTGGCTT128940.13636701107075072No Hit
TGTAAACATCCCCGACTGGAAGC127320.13465369822807494No Hit
GGAATACCGGGTGCTGTAGGCTTT124530.13170299277679998No Hit
AACATTCAACGCTGTCGGTGA123960.13110016048030293No Hit
CAAAGAATTCTCCTTTTGGGCTT122730.12979931184049354No Hit
TGTCAGTTTGTCAAATACCCC121100.12807542299261604No Hit
TGAGGTAGTAGGTTGTATAGT120050.1269649424464373No Hit
CACCCGGCTGTGTGCACATGTGC119850.12675342234240325No Hit
CAAAGTGCTGTTCGTGCAGGT117690.12446900521883553No Hit
ACTGGACTTGGAGTCAGAAGG117670.12444785320843213No Hit
TAGCAGCACATCATGGTTTACA116270.1229672124801938No Hit
CCCATAAAGTAGAAAGCACTA115210.12184615592881333No Hit
ATCACATTGCCAGGGATTTCC115130.12176154788719971No Hit
ATCCCACCGCTGCCACCA114790.12140196371034184No Hit
TGAGAACTGAATTCCATAGGCTGT113930.12049242726299542No Hit
TGAGGTAGTAGGTTGTGTGGTT110490.11685428147360981No Hit
ACCACAGGGTAGAACCACGGAC110020.1163572092291298No Hit
TAGCACCATCTGAAATCGGTTA108670.11492944852689996No Hit
ACTGGACTTGGAGTCAGAAGGCA106140.11225371921086925No Hit
TGTCAGTTTGTCAAATACCC105710.11179895098719607No Hit
CACTAGATTGTGAGCTCCTGGA102500.10840405331744958No Hit
TGTAAACATCCTTGACTGGAAGC101440.10728299676606913No Hit
TCGTTTCCCGGCCAATGCACCA100010.10577062802222569No Hit
CATAAAGTAGAAAGCACTAC99710.10545334786617462No Hit
GGGCTACGCCTGTCTGAGCGTCGCT99340.10506203567371164No Hit
CAAAGTGCTGTTCGTGCAGGTAG99210.1049245476060895No Hit
AGAGGTAGTAGGTTGCATAGTT98220.10387752309112097No Hit
TAGCTTATCAGACTGATGTTG97120.1027141625189337No Hit
GGAATACCGGGTGCTGTAGGCTT95630.10113833774388004No Hit
CCCCCCACTGCTAAATTTGACTGG95180.10066241750980341No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)