FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10546409
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6327475.999644049457972No Hit
TTCAAGTAATCCAGGATAGGCT5367045.088973886751405No Hit
AAGCTGCCAGTTGAAGAACTGT4938574.682702899157428No Hit
TAGCAGCACGTAAATATTGGCG4513314.279475601600507No Hit
AACATTCAACGCTGTCGGTGAGT2916812.765690198436264No Hit
CATAAAGTAGAAAGCACTACT2827202.680722888710271No Hit
CCCCCCACTGCTAAATTTGACTG2776692.6328298096536935No Hit
TAGCTTATCAGACTGATGTTGAC2243152.1269324942736434No Hit
TAGCTTATCAGACTGATGTTGA2180612.0676326890034322No Hit
TGAGGTAGTAGGTTGTATAGTT2139072.0282448746298387No Hit
AACATTCAACGCTGTCGGTGAG1790201.6974498144344676No Hit
GCCTGTCTGAGCGTCGCT1765471.6740010746785945No Hit
TCCACCACGTTCCCGTGG1756001.6650217149742625No Hit
TGAGGTAGTAGATTGTATAGTT1520431.4416565866163544No Hit
TAGCTTATCAGACTGATGTTGACT1457611.3820912881341887No Hit
CCCCCACTGCTAAATTTGACTG1086291.0300093614802914No Hit
TTCACAGTGGCTAAGTTCCGC886850.8409023393649915No Hit
TTCAAGTAATTCAGGATAGGTT864200.819425834897926No Hit
TGTAAACATCCTTGACTGGAAGCT840210.7966787557736477No Hit
CGCCTGTCTGAGCGTCGCT737390.6991858555836399No Hit
TATTGCACTTGTCCCGGCCTGT735670.6975549687102026No Hit
TGAGGTAGTAGTTTGTACAGTT715840.67875236016354No Hit
TTCACAGTGGCTAAGTTCCG689810.6540709733521619No Hit
TATTGCACTTGTCCCGGCCTGTAA610080.5784717812480058No Hit
CCCATAAAGTAGAAAGCACTAC580770.5506803310965847No Hit
AGCAGCATTGTACAGGGCTATGA571320.5417199351931069No Hit
TCGTACGACTCTTAGCGG562170.533043996302438No Hit
TATTGCACTTGTCCCGGCCTGTAT547570.5192004216790758No Hit
TAGCAGCACATCATGGTTTACA529280.5018580257981651No Hit
CATTGCACTTGTCTCGGTCTGA471150.44673973861624366No Hit
TCCTGTCTGAGCGTCGCT463970.43993173410968606No Hit
CTACGCCTGTCTGAGCGTCGCT454570.4310187477083432No Hit
CAACACCAGTCGATGGGCTGT433860.41138173192410804No Hit
AACCGGGCGGAAACACCA427420.4052753880491454No Hit
TGAGGTAGTAGTTTGTGCTGTT426880.40476336542608954No Hit
TATTGCACTTGTCCCGGCCTGTA387440.36736675014215736No Hit
CAACGGAATCCCAAAAGCAGCTGT370570.35137078412187506No Hit
TGTAAACATCCTACACTCTCAGCT364130.3452644402469125No Hit
ACTGGACTTGGAGTCAGAAGGC323920.3071377186301043No Hit
TACGCCTGTCTGAGCGTCGCT320600.3039897276883535No Hit
TTCTCACTACTGCACTTGACTAG301210.28560432276047704No Hit
TGTCAGTTTGTCAAATACCCCA281980.26737062823943203No Hit
TCAGGCTCAGTCCCCTCCCGAT281710.2671146169279041No Hit
TGTAAACATCCCCGACTGGAAGCT278190.263776987977614No Hit
CCCATAAAGTAGAAAGCACT276250.26193749929478366No Hit
TGTCAGTTTGTCAAATACC264230.2505402549815771No Hit
TAGCAGCACATAATGGTTTGT262690.24908004231582523No Hit
TTCAAGTAATCCAGGATAGGC261540.24798962376672476No Hit
TCGTTTCCCGGCCAATGCACCA259870.24640614639542235No Hit
TTCACAGTGGCTAAGTTCTGC258100.24472785001985034No Hit
TGTCAGTTTGTCAAATACCCCAA250620.23763538850048394No Hit
ACGCCTGTCTGAGCGTCGCT250620.23763538850048394No Hit
TGTAAACATCCTACACTCAGCT247120.23431672335104772No Hit
CCCCCACTGCTAAATTTGACTGGCTT243390.2307799744917915No Hit
TAGCAGCACGTAAATATTGGC232920.22085242474476383No Hit
TGAGGTAGTAGGTTGTATAGT230700.2187474428499786No Hit
TTATAAAGCAATGAGACTGATT219210.207852739259401No Hit
TAGCAGCACGTAAATATTGGCGT217130.2058805039705932No Hit
ACCGGGTGCTGTAGGCTT208140.19735627548675572No Hit
ATCACATTGCCAGGGATTTCC207250.19651238634875623No Hit
AACATTCAACGCTGTCGGTGAGTT203730.19317475739846615No Hit
TAGCTTATCAGACTGATGTTGACA198840.18853810808968247No Hit
TCAGGCTCAGTCCCCTCCCGA193550.18352218276382035No Hit
TAGCACCATCTGAAATCGGTTA176810.1676494814490885No Hit
TGAGGTAGTAGATTGTATAGT174910.165847920367966No Hit
TTCACAGTGGCTAAGTTCTG172370.16343951765951803No Hit
GTCAGGATGGCCGAGCGGTCTAAGG170200.1613819452668676No Hit
AACGGAATCCCAAAAGCAGCTG167760.15906836156268928No Hit
GTCAAGTAATCCAGGATAGGCT165530.15695389776747706No Hit
TTCACAGTGGCTAAGTTCCGCT163570.1550954452837928No Hit
CTTCTCACTACTGCACTTGACTAG155400.14734873263496606No Hit
TGTCAGTTTGTCAAATACCC153610.14565147245854015No Hit
CAAAGAATTCTCCTTTTGGGCT150800.14298705843856424No Hit
CAAAGTGCTGTTCGTGCAGGT148550.14085363084249813No Hit
TTCAAGTAATCCAGGATAGGCTT146810.139203780168207No Hit
TGAGGTAGTAGGTTGTGTGGTT145910.13835040912978058No Hit
CTCCCACTGCTTCACTTGACTAG145480.13794268741142127No Hit
AGCTCGGTCTGAGGCCCCTCAGT145380.13784786840715166No Hit
ATCCCGGACGAGCCCCCA139210.13199753584371704No Hit
AGAGGTAGTAGGTTGCATAGTT138480.13130535711254893No Hit
AGAATTACTCTGAGACCT138200.13103986390059402No Hit
ATCCCACCGCTGCCACCA136470.12939949512672985No Hit
TAGCTTATCAGACTGATGTTG136300.12923830281947155No Hit
GAGCAGCACGTAAATATTGGCG136140.12908659241264017No Hit
CATAAAGTAGAAAGCACTAC134270.12731347703279855No Hit
TGTCAGTTTGTCAAATACCCC133110.12621357658327115No Hit
CAACGGAATCCCAAAAGCAGCT132620.12574896346235007No Hit
ACCGGGTGCTGTAGGCTTT132090.12524642273972117No Hit
CCCCCCACTGCTAAATTTGACTGGCT132070.12522745893886725No Hit
TGAGGTAGTAAGTTGTATTGTT127920.12129247026167864No Hit
GGGCTACGCCTGTCTGAGCGTCGCT125200.1187133933455454No Hit
ACTGGACTTGGAGTCAGAAGG125110.11862805624170275No Hit
AGCAGCATTGTACAGGGCTATG124030.11760401099559102No Hit
CCCCCCACTGCTAAATTTGACTGG119980.113763841322672No Hit
TATTGCACTTGTCCCGGCCTGTT117530.1114407757180667No Hit
TCGACCGGACCTCGACCGGCT116120.11010382775786527No Hit
CCCATAAAGTAGAAAGCACTA115940.10993315355017996No Hit
AGCAGCATTGTACAGGGCTATGT115610.10962025083609027No Hit
CACCCGGCTGTGTGCACATGTGC109880.10418712189144191No Hit
ACCGGGTGCTGTAGGCTTA107900.10230970560690374No Hit
AACATTCAACGCTGTCGGTGA106310.10080208343901702No Hit
TCAGGCTCAGTCCCCTCCCGATA106030.10053659022706213No Hit
TAGCAGCACGTAAATATTGGCGA105980.10048918072492732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)