FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10183370
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8262188.113404501653186No Hit
CCCCCCACTGCTAAATTTGACTG5805245.700706151303547No Hit
TTCAAGTAATCCAGGATAGGCT3801663.733204233961842No Hit
AACATTCAACGCTGTCGGTGAGT3392263.3311762216240792No Hit
TAGCAGCACGTAAATATTGGCG2960042.9067391246709096No Hit
AAGCTGCCAGTTGAAGAACTGT2810742.760127541275629No Hit
AACATTCAACGCTGTCGGTGAG2407662.3643057259040963No Hit
TGAGGTAGTAGGTTGTATAGTT1865331.8317413587054188No Hit
TAGCTTATCAGACTGATGTTGA1847811.8145368380015654No Hit
TAGCTTATCAGACTGATGTTGACT1840961.8078101846441799No Hit
TAGCTTATCAGACTGATGTTGAC1817631.7849002835014343No Hit
CCCCCACTGCTAAATTTGACTG1670471.6403901655345923No Hit
TCGTACGACTCTTAGCGG1507391.4802467159692716No Hit
TATTGCACTTGTCCCGGCCTGTAA1340051.3159199754108906No Hit
TATTGCACTTGTCCCGGCCTGTAT1277851.2548399989394474No Hit
TGAGGTAGTAGATTGTATAGTT1170701.1496194285388825No Hit
TTCAAGTAATTCAGGATAGGTT1153431.1326604061327439No Hit
CATAAAGTAGAAAGCACTACT998650.9806675000515547No Hit
TATTGCACTTGTCCCGGCCTGT888550.8725500497379551No Hit
TGTAAACATCCTTGACTGGAAGCT835450.8204062113033308No Hit
TTAATGCTAATCGTGATAGGGGTT823220.8083964345791226No Hit
AGCAGCATTGTACAGGGCTATGA621430.6102400285956417No Hit
TATTGCACTTGTCCCGGCCTGTA606370.595451211141302No Hit
CCCATAAAGTAGAAAGCACTAC505600.4964957572984189No Hit
CAACGGAATCCCAAAAGCAGCTGT495320.4864008672963861No Hit
TGTAAACATCCCCGACTGGAAGCT487670.4788886193863132No Hit
TGAGGTAGTAGTTTGTACAGTT480830.47217178596083614No Hit
CACCCGGCTGTGTGCACATGTGC462980.4546432075039992No Hit
ACTGGACTTGGAGTCAGAAGGC418430.4108954108512212No Hit
CCCATAAAGTAGAAAGCACT365590.35900689064622027No Hit
CAACGGAATCCCAAAAGCAGCT353130.34677125548811444No Hit
CATTGCACTTGTCTCGGTCTGA332500.32651273596068886No Hit
CAACACCAGTCGATGGGCTGT319900.31413962175586274No Hit
CAAAGAATTCTCCTTTTGGGCT293090.28781238430892725No Hit
TGTAAACATCCTACACTCTCAGCT291200.2859564171782033No Hit
TAGCTTATCAGACTGATGTTGACA285090.2799564387820535No Hit
TTCTCACTACTGCACTTGACTAG276710.27172733584265324No Hit
CTCCCACTGCTTCACTTGACTAG264380.25961935979935913No Hit
TGAGGTAGTAGTTTGTGCTGTT263070.25833294871933354No Hit
CCCCCACTGCTAAATTTGACTGGCTT257010.2523820699827267No Hit
TGAGAACTGAATTCCATGGGTT252810.24825769858111804No Hit
AACATTCAACGCTGTCGGTGAGTT220790.216814276609806No Hit
CTTCTCACTACTGCACTTGACTAG215240.21136421440053735No Hit
AACGGAATCCCAAAAGCAGCTG205670.20196653956401464No Hit
TTCAAGTAATCCAGGATAGGC204450.20076850787116643No Hit
TTCAAGTAATCCAGGATAGGCTT204190.20051318964154305No Hit
ACCGGGTGCTGTAGGCTT199430.19583890205305318No Hit
CGCCTGTCTGAGCGTCGCT197630.19407131430950658No Hit
TAGCAGCACGTAAATATTGGC197180.19362941737361994No Hit
TCGTTTCCCGGCCAATGCACCA193070.18959342535918855No Hit
GCCTGTCTGAGCGTCGCT190180.18675546503760543No Hit
ATCCCGGACGAGCCCCCA186620.18325956927814663No Hit
TTCACAGTGGCTAAGTTCCG186550.18319082975478648No Hit
TGAGGTAGTAGGTTGTATAGT177940.17473586838148866No Hit
TATTGCACTTGTCCCGGCCTGTT174370.17123015269012123No Hit
AGCTCGGTCTGAGGCCCCTCAGT170550.16747893870103905No Hit
AGCAGCATTGTACAGGGCTATGT167310.1642972807626552No Hit
CCCCCCACTGCTAAATTTGACTGGCT166080.16308942913789837No Hit
TACGCCTGTCTGAGCGTCGCT164210.16125310187099162No Hit
TGAGGTAGTAGGTTGTGTGGTT162520.15959353337843954No Hit
CAAAGAATTCTCCTTTTGGGCTT160340.15745278822236647No Hit
TATTGCACTTGTCCCGGCCTGTAG152000.14926296501060063No Hit
ACCGGGTGCTGTAGGCTTA151980.14924332514678343No Hit
CCCCCCACTGCTAAATTTGACTGG148130.14546265136197545No Hit
TTCACAGTGGCTAAGTTCCGC147450.14479489599219117No Hit
ACCGGGTGCTGTAGGCTTT145870.14324334675063363No Hit
ACCCCCCACTGCTAAATTTGACTG145720.14309604777200474No Hit
TTATAAAGCAATGAGACTGATT143760.1411713411179207No Hit
TCGTTTCCCGGGCGGCGCACCA143240.14066070465867392No Hit
CAAAGTGCTGTTCGTGCAGGTAG135690.13324665606768682No Hit
CTACGCCTGTCTGAGCGTCGCT134790.13236286219591353No Hit
CCCATAAAGTAGAAAGCACTA132000.12962310119341633No Hit
TTCACAGTGGCTAAGTTCTG126540.12426141837132501No Hit
CTCCCACTGCTTCACTTGACTAGCCT125020.122768788721219No Hit
TTAATGCTAATCGTGATAGGGGTTT119860.11770170385638547No Hit
TGTCAGTTTGTCAAATACCCCA118370.11623853400200522No Hit
CAAAGTGCTGTTCGTGCAGGT117040.11493248305816248No Hit
TTCACAGTGGCTAAGTTCTGC115640.11355769259095956No Hit
TGTAAACATCCCCGACTGGAAGC115340.1132630946337018No Hit
AGAGGTAGTAGGTTGCATAGTT114560.11249713994483163No Hit
CGCGACCTCAGATCAGACGTGGCG113570.111524966685881No Hit
ATCACATTGCCAGGGATTTCC113400.11135802784343495No Hit
CACTAGATTGTGAGCTCCTGGA112620.11059207315456475No Hit
TATTGCACTTGTCCCGGCCTGTTT112140.11012071642295233No Hit
ATGCACCTGGGCAAGGATTCTGA110470.10848078779421744No Hit
TGAGGTAGTAAGTTGTATTGTT107950.10600616495325221No Hit
TATTGCACTTGTCCCGGCCTGTAAT106970.10504381162621018No Hit
TGAGGTAGTAGATTGTATAGT105710.10380650020572757No Hit
TAGCTTATCAGACTGATGTTG102690.10084088076933274No Hit
TGATGTAGTAGGTTGTATAGTT102530.10068376185879528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)