FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11151342
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8526477.646138016393005No Hit
CCCCCCACTGCTAAATTTGACTG5556574.9828711199064655No Hit
AACATTCAACGCTGTCGGTGAGT3904873.5017040998294195No Hit
AAGCTGCCAGTTGAAGAACTGT3543663.177787929022354No Hit
TTCAAGTAATCCAGGATAGGCT3508053.1458545527524846No Hit
TAGCAGCACGTAAATATTGGCG3245792.910672096685762No Hit
AACATTCAACGCTGTCGGTGAG2731022.449050526833452No Hit
TAGCTTATCAGACTGATGTTGACT2595592.327603260665846No Hit
TAGCTTATCAGACTGATGTTGAC2536752.274838310940513No Hit
TAGCTTATCAGACTGATGTTGA2081551.8666363205433034No Hit
TATTGCACTTGTCCCGGCCTGTAT1967421.764289894436024No Hit
TATTGCACTTGTCCCGGCCTGTAA1947651.746561086549045No Hit
CCCCCACTGCTAAATTTGACTG1664781.4928965500295839No Hit
TGAGGTAGTAGGTTGTATAGTT1440411.2916920671969347No Hit
TTAATGCTAATCGTGATAGGGGTT1203291.0790539829197239No Hit
TATTGCACTTGTCCCGGCCTGT1196361.0728394842522093No Hit
TGAGGTAGTAGATTGTATAGTT1079270.9678386691036828No Hit
TGTAAACATCCTTGACTGGAAGCT1066940.9567817039420008No Hit
TTCAAGTAATTCAGGATAGGTT953420.8549822971979516No Hit
CATAAAGTAGAAAGCACTACT943180.8457995459201234No Hit
AGCAGCATTGTACAGGGCTATGA845980.7586351490251129No Hit
TATTGCACTTGTCCCGGCCTGTA799450.7169092294003717No Hit
TGTAAACATCCCCGACTGGAAGCT677330.6073977463878338No Hit
TCGTACGACTCTTAGCGG649780.5826921997370361No Hit
CCCATAAAGTAGAAAGCACTAC585650.5251834263535277No Hit
GGAATACCGGGTGCTGTAGGCTTT546920.4904521805536948No Hit
ACTGGACTTGGAGTCAGAAGGC539380.4836906625229501No Hit
CAACGGAATCCCAAAAGCAGCTGT529140.47450791124512187No Hit
CACCCGGCTGTGTGCACATGTGC505540.45334453915950207No Hit
CAACGGAATCCCAAAAGCAGCT414410.37162343330515735No Hit
CCCATAAAGTAGAAAGCACT400130.3588177996872484No Hit
TAGCTTATCAGACTGATGTTGACA398910.3577237609607884No Hit
TGAGGTAGTAGTTTGTACAGTT395680.3548272485948328No Hit
GGAATACCGGGTGCTGTAGGCTT381820.3423982512598035No Hit
TGAGAACTGAATTCCATGGGTT380910.3415822059802309No Hit
CATTGCACTTGTCTCGGTCTGA364890.3272162220475347No Hit
CAAAGAATTCTCCTTTTGGGCT363430.3259069625880006No Hit
TTCTCACTACTGCACTTGACTAG344960.30934393367183965No Hit
TGTAAACATCCTACACTCTCAGCT311490.27932960893854747No Hit
CAACACCAGTCGATGGGCTGT304300.27288195447686925No Hit
TCGTTTCCCGGCCAATGCACCA294610.26419241737900245No Hit
ATCCCGGACGAGCCCCCA294420.2640220342986521No Hit
CTTCTCACTACTGCACTTGACTAG278160.24944082963288186No Hit
AACATTCAACGCTGTCGGTGAGTT263330.23614198183501142No Hit
TTAATGCTAATCGTGATAGGGGTTT258820.23209762555932728No Hit
CCCCCACTGCTAAATTTGACTGGCTT255910.22948807417080383No Hit
TGAGGTAGTAGTTTGTGCTGTT248540.22287900415932002No Hit
TGAGAACTGAATTCCATAGGCTGT238000.21342722696514912No Hit
TATTGCACTTGTCCCGGCCTGTAG235880.21152611048966125No Hit
TATTGCACTTGTCCCGGCCTGTT232990.20893449416222726No Hit
CAAAGAATTCTCCTTTTGGGCTT227430.20394854717934396No Hit
TGATGTAGTAGGTTGTATAGTT226960.20352707324374056No Hit
TTCAAGTAATCCAGGATAGGCTT216830.19444296480190457No Hit
TAGCAGCACGTAAATATTGGC210600.1888561932725227No Hit
CTCCCACTGCTTCACTTGACTAG208840.187277907896646No Hit
TGTAAACATCCCCGACTGGAAGC199680.17906364991765117No Hit
AGCTCGGTCTGAGGCCCCTCAGT191890.17207794362328765No Hit
TATTGCACTTGTCCCGGCCTGTTT189400.16984502851764388No Hit
AACGGAATCCCAAAAGCAGCTG188740.16925317150169011No Hit
TCGTTTCCCGGGCGGCGCACCA184720.16564822422269893No Hit
TGAATACCGGGTGCTGTAGGCTTT183860.16487701659585008No Hit
ACTGGACTTGGAGTCAGAAGGCA183480.1645362504351494No Hit
AGCAGCATTGTACAGGGCTATGT181750.16298486765090694No Hit
TTCACAGTGGCTAAGTTCCG175030.15695868712483216No Hit
TTCAAGTAATCCAGGATAGGC174380.15637579763942314No Hit
TGATGTAGTAGATTGTATAGTT168390.15100424684311536No Hit
TTATAAAGCAATGAGACTGATT167870.15053793525478817No Hit
TTCACAGTGGCTAAGTTCCGC167700.15038548723552736No Hit
CGCCTGTCTGAGCGTCGCT165310.1482422474353311No Hit
TGAGGTAGTAGGTTGTATAGT160940.14432343658727353No Hit
TATTGCACTTGTCCCGGCCTGTAAT157500.1412386060798781No Hit
ATGCACCTGGGCAAGGATTCTGA143740.12889928405029638No Hit
CAAAGTGCTGTTCGTGCAGGTAG143340.1285405828285062No Hit
TATTGCACTTGTCCCGGCCTGTAAA142660.1279307907514629No Hit
CCCATAAAGTAGAAAGCACTA141470.12686365461663718No Hit
ACCGGGTGCTGTAGGCTT141040.12647805080321275No Hit
CGCGACCTCAGATCAGAC139220.12484596024406748No Hit
TGTCAGTTTGTCAAATACCCCAA138870.12453209667500108No Hit
CCCCCCACTGCTAAATTTGACTGG138400.12411062273939766No Hit
TTAATGCTAATCGTGATAGGGGT136350.12227227897772305No Hit
TGTCAGTTTGTCAAATACCCCA136210.12214673355009649No Hit
ACCCCCCACTGCTAAATTTGACTG133540.11975240289464713No Hit
TGAGGTAGTAGGTTGTGTGGTT133270.11951027956993876No Hit
CTACGCCTGTCTGAGCGTCGCT133060.11932196142849891No Hit
ACTGGACTTGGAGTCAGAAGGCT130740.11724149434211595No Hit
TGAATACCGGGTGCTGTAGGCTT130150.11671241003997546No Hit
CCCCCCACTGCTAAATTTGACTGGCT129450.11608468290184266No Hit
TTCACAGTGGCTAAGTTCTGC128720.11543005317207562No Hit
ACCGGGTGCTGTAGGCTTA123130.11041720359755804No Hit
ACTTGACTTGGAGTCAGAAGGC122340.10970876868452246No Hit
ACTGGACTTGGAGTCAGAAGG121390.1088568532827708No Hit
TACGCCTGTCTGAGCGTCGCT121310.10878511303841276No Hit
AGCTACATCTGGCTACTGGGTCTC120200.10778971714794507No Hit
ATCACATTGCCAGGGATTTCC119280.10696470433782769No Hit
GCCTGTCTGAGCGTCGCT113680.10194288723276534No Hit
ACCACAGGGTAGAACCACGGAC113290.10159315354151995No Hit
TGAGGTAGTAGATTGTATAGT112140.10056188752887321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)