FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7454906
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5223617.006942810546504No Hit
CCCCCCACTGCTAAATTTGACTG4063815.451188787625223No Hit
AACATTCAACGCTGTCGGTGAGT2984984.004047804224493No Hit
TTCAAGTAATCCAGGATAGGCT2496413.3486807211251217No Hit
AACATTCAACGCTGTCGGTGAG2326133.120267378287533No Hit
AAGCTGCCAGTTGAAGAACTGT2205742.9587764084483426No Hit
TAGCAGCACGTAAATATTGGCG2144642.8768169578529896No Hit
CCCCCACTGCTAAATTTGACTG1528742.0506495990693914No Hit
TAGCTTATCAGACTGATGTTGA1406331.8864490041859683No Hit
TAGCTTATCAGACTGATGTTGAC1337271.7938120212380948No Hit
TAGCTTATCAGACTGATGTTGACT1188691.5945070266479549No Hit
TGAGGTAGTAGGTTGTATAGTT1042861.3988908780338747No Hit
CGCCTGTCTGAGCGTCGCT810581.0873108259178585No Hit
TATTGCACTTGTCCCGGCCTGT796251.0680885848862482No Hit
TATTGCACTTGTCCCGGCCTGTAA673490.9034185005149629No Hit
CATAAAGTAGAAAGCACTACT671700.9010173971341825No Hit
TATTGCACTTGTCCCGGCCTGTAT605510.8122302279867781No Hit
TGTAAACATCCTTGACTGGAAGCT569630.7641008484882303No Hit
TTAATGCTAATCGTGATAGGGGTT546010.7324170150502233No Hit
TGAGGTAGTAGATTGTATAGTT530670.7118399614964963No Hit
CAACACCAGTCGATGGGCTGT493000.6613094786171683No Hit
TATTGCACTTGTCCCGGCCTGTA490830.6583986437924234No Hit
TCGTACGACTCTTAGCGG480860.6450249003810377No Hit
AGCAGCATTGTACAGGGCTATGA471690.632724275799051No Hit
TTCAAGTAATTCAGGATAGGTT393900.5283768836253602No Hit
ACCGGGTGCTGTAGGCTT392620.5266598934983218No Hit
TGTAAACATCCCCGACTGGAAGCT382180.512655692774664No Hit
ACTGGACTTGGAGTCAGAAGGC378820.508148593691188No Hit
CATTGCACTTGTCTCGGTCTGA314340.42165521604162415No Hit
CCCATAAAGTAGAAAGCACT302920.4063364447519526No Hit
CAACGGAATCCCAAAAGCAGCT277660.3724527177136774No Hit
CACCCGGCTGTGTGCACATGTGC262830.3525597774136924No Hit
ACCGGGTGCTGTAGGCTTT250550.3360874033824169No Hit
TTCACAGTGGCTAAGTTCCG239840.32172102505383704No Hit
CCCATAAAGTAGAAAGCACTAC235230.315537177799425No Hit
CAACGGAATCCCAAAAGCAGCTGT230780.30956795431089273No Hit
TGAGGTAGTAGTTTGTACAGTT225090.301935396636792No Hit
AACATTCAACGCTGTCGGTGAGTT216630.29058716501589693No Hit
GGAATACCGGGTGCTGTAGGCTTT210290.2820826982929094No Hit
TACGCCTGTCTGAGCGTCGCT199210.26722000250573247No Hit
ACCGGGTGCTGTAGGCTTA192190.2578033847777557No Hit
TGAGGTAGTAGTTTGTGCTGTT189270.2538865010504492No Hit
TTCACAGTGGCTAAGTTCTG182480.2447784049859247No Hit
GGAATACCGGGTGCTGTAGGCTT178970.24007009612193636No Hit
TTCTCACTACTGCACTTGACTAG177860.23858114374614514No Hit
ATCCCGGACGAGCCCCCA177490.2380848262875481No Hit
CTACGCCTGTCTGAGCGTCGCT174700.23434232437001887No Hit
TAGCTTATCAGACTGATGTTGACA162670.21820529997293056No Hit
AACGGAATCCCAAAAGCAGCTG158090.21206169467462097No Hit
TAGCAGCACGTAAATATTGGC150800.20228289934172208No Hit
ACTGGACTTGGAGTCAGAAGG148160.1987416072047052No Hit
ACGCCTGTCTGAGCGTCGCT147690.19811114989243325No Hit
TCGTTTCCCGGCCAATGCACCA146360.1963270898385573No Hit
TGTCAGTTTGTCAAATACCCCAA145450.1951064171701159No Hit
TGAGAACTGAATTCCATGGGTT145200.19477106753592868No Hit
CAAAGAATTCTCCTTTTGGGCT140850.18893598390107133No Hit
TATTGCACTTGTCCCGGCCTGTT136880.1836106317101785No Hit
TGTAAACATCCTACACTCTCAGCT134520.18044493116345128No Hit
TGAGGTAGTAGGTTGTGTGGTT133440.17899622074376256No Hit
ACTGGACTTGGAGTCAGAAGGCT122850.16479081023959255No Hit
AGCAGCATTGTACAGGGCTATG121490.16296650822961417No Hit
TTCAAGTAATCCAGGATAGGC118630.15913010841451253No Hit
CTTCTCACTACTGCACTTGACTAG118100.15841916719003565No Hit
ACTGGACTTGGAGTCAGAAGGCA117420.15750701618504648No Hit
TTATAAAGCAATGAGACTGATT116100.15573637011653804No Hit
ACCACAGGGTAGAACCACGGAC111550.1496330067743309No Hit
TGAGGTAGTAGGTTGTATAGT111110.1490427914181614No Hit
GCCTGTCTGAGCGTCGCT103790.13922375412916005No Hit
GCGTTCGCGCTTTCCCCTG103460.13878109261203292No Hit
CTCCCACTGCTTCACTTGACTAG101350.13595074169949292No Hit
TTCAAGTAATCCAGGATAGGCTT99600.1336032942601825No Hit
TGTAAACATCCCCGACTGGAAGC94190.12634632817637137No Hit
AGCTACATTGTCTGCTGGGTTT93770.12578294079093685No Hit
CAAAGTGCTGTTCGTGCAGGTAG93260.12509882753719495No Hit
AGCAGCATTGTACAGGGCTATGT91650.12293917589302937No Hit
CCCCCCACTGCTAAATTTGACTGG91400.12260382625884216No Hit
AATGCACCCGGGCAAGGATTCT91170.12229530459538993No Hit
CGCGACCTCAGATCAGACGTGG88800.11911619006329524No Hit
TGATGTAGTAGGTTGTATAGTT88670.11894180825351788No Hit
TGTCAGTTTGTCAAATACCCCA87020.11672850066788235No Hit
TATTGCACTTGTCCCGGCCTGTAG87000.11670167269714736No Hit
TGAGAACTGAATTCCATAGGCTGT86700.1162992531361227No Hit
CCCATAAAGTAGAAAGCACTA85010.11403228960901719No Hit
TTCACAGTGGCTAAGTTCTGC84870.11384449381387238No Hit
TGAATACCGGGTGCTGTAGGCTTT84020.11270430505763587No Hit
TAGCTTATCAGACTGATGTTG83010.11134949253551957No Hit
CAAAGTGCTGTTCGTGCAGGT82910.11121535268184468No Hit
TATTGCACTTGTCCCGGCCTG79950.10724481301306818No Hit
CCCCCACTGCTAAATTTGACTGGCTT79270.10633266200807898No Hit
AACATTCAACGCTGTCGGTGA76960.10323403138818919No Hit
TGAGGTAGTAAGTTGTATTGTT76700.1028852677686345No Hit
TTCACAGTGGCTAAGTTCCGC76080.10205360067585025No Hit
TTAATGCTAATCGTGATAGGGGT75850.10174507901239802No Hit
CAAAGAATTCTCCTTTTGGGCTT74900.10047075040248664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)