FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10916007
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7982307.312472408638067No Hit
CCCCCCACTGCTAAATTTGACTG5701525.223082029903425No Hit
AACATTCAACGCTGTCGGTGAGT4237083.881529207520662No Hit
TTCAAGTAATCCAGGATAGGCT3556863.2583892626672No Hit
TAGCAGCACGTAAATATTGGCG3417983.1311632541093095No Hit
AAGCTGCCAGTTGAAGAACTGT3216462.946553625332047No Hit
AACATTCAACGCTGTCGGTGAG3112452.8512715317972956No Hit
TAGCTTATCAGACTGATGTTGA2337062.140947692686529No Hit
TAGCTTATCAGACTGATGTTGAC2134971.9558158949513313No Hit
TAGCTTATCAGACTGATGTTGACT2109491.9324740264457507No Hit
CCCCCACTGCTAAATTTGACTG1920191.7590589672578993No Hit
TGAGGTAGTAGGTTGTATAGTT1597991.4638960931410177No Hit
TATTGCACTTGTCCCGGCCTGTAA1551061.4209041822710446No Hit
TATTGCACTTGTCCCGGCCTGTAT1459721.3372288969767059No Hit
CATAAAGTAGAAAGCACTACT1133891.0387406310750809No Hit
TGAGGTAGTAGATTGTATAGTT1122901.0286728471317397No Hit
TATTGCACTTGTCCCGGCCTGT1115131.021554859757785No Hit
TGTAAACATCCTTGACTGGAAGCT1020080.9344808958074139No Hit
TCGTACGACTCTTAGCGG973020.8913698937715961No Hit
TTCAAGTAATTCAGGATAGGTT911570.8350764157626502No Hit
AGCAGCATTGTACAGGGCTATGA858940.7868628153133285No Hit
TATTGCACTTGTCCCGGCCTGTA788280.7221321862472239No Hit
TGTAAACATCCCCGACTGGAAGCT655300.6003110844469044No Hit
ACTGGACTTGGAGTCAGAAGGC565990.5184954535115267No Hit
CCCATAAAGTAGAAAGCACTAC485740.4449795607496404No Hit
TTCTCACTACTGCACTTGACTAG462100.4233232902837091No Hit
TGAGGTAGTAGTTTGTACAGTT438600.40179527184253366No Hit
CCCATAAAGTAGAAAGCACT437930.4011814942954874No Hit
CAACGGAATCCCAAAAGCAGCT429390.3933581207853751No Hit
CAACACCAGTCGATGGGCTGT425550.3898403509634979No Hit
CATTGCACTTGTCTCGGTCTGA420910.3855897124287297No Hit
CAACGGAATCCCAAAAGCAGCTGT419580.38437131819354825No Hit
CACCCGGCTGTGTGCACATGTGC409280.37493563351507564No Hit
CTTCTCACTACTGCACTTGACTAG332710.3047909368324883No Hit
CCCCCACTGCTAAATTTGACTGGCTT331930.3040763898374195No Hit
CGCCTGTCTGAGCGTCGCT328190.3006502286046537No Hit
TAGCTTATCAGACTGATGTTGACA318610.29187412576778304No Hit
TTCACAGTGGCTAAGTTCCG316000.28948314159197586No Hit
AACATTCAACGCTGTCGGTGAGTT315380.28891516833948533No Hit
TCGTTTCCCGGCCAATGCACCA306730.2809910253813505No Hit
CAAAGAATTCTCCTTTTGGGCT305270.27965353998032433No Hit
TACGCCTGTCTGAGCGTCGCT297710.27272793064350365No Hit
GCCTGTCTGAGCGTCGCT294760.27002547726471776No Hit
TGAGGTAGTAGTTTGTGCTGTT284170.2603241276778221No Hit
GGAATACCGGGTGCTGTAGGCTTT284060.2602233582297996No Hit
TGTAAACATCCTACACTCTCAGCT269650.24702256053884905No Hit
ATCCCGGACGAGCCCCCA265530.24324828666745998No Hit
CTCCCACTGCTTCACTTGACTAG259550.23777009303859917No Hit
TGTCAGTTTGTCAAATACCCCAA253960.2326491729072728No Hit
ACCGGGTGCTGTAGGCTT230410.21107535017154166No Hit
CTACGCCTGTCTGAGCGTCGCT226770.20774079752788727No Hit
TATTGCACTTGTCCCGGCCTGTT220980.2024366602183381No Hit
GGAATACCGGGTGCTGTAGGCTT220380.2018870086836698No Hit
TAGCAGCACGTAAATATTGGC220120.20164882635198017No Hit
AACGGAATCCCAAAAGCAGCTG219100.20071441874304402No Hit
TGTAAACATCCCCGACTGGAAGC204010.18689068264613606No Hit
AGCAGCATTGTACAGGGCTATGT197380.18081703318805126No Hit
ACTGGACTTGGAGTCAGAAGGCA192330.17619079943792634No Hit
TTATAAAGCAATGAGACTGATT190440.17445939710372116No Hit
TTCAAGTAATCCAGGATAGGC188460.17264554703931576No Hit
TGTCAGTTTGTCAAATACCCCA185520.16995225451944104No Hit
CGTTCGCGCTTTCCCCTG184850.16933847697239476No Hit
TGAGGTAGTAGGTTGTATAGT179980.16487713868267034No Hit
TTCAAGTAATCCAGGATAGGCTT177090.1622296504573513No Hit
TTCACAGTGGCTAAGTTCTG176950.16210139843259536No Hit
TATTGCACTTGTCCCGGCCTGTAG172320.15785992075673824No Hit
ACCGGGTGCTGTAGGCTTA170090.15581704921955436No Hit
ACTGGACTTGGAGTCAGAAGGCT167230.15319704357096875No Hit
CCCATAAAGTAGAAAGCACTA163880.15012815583573738No Hit
ACTGGACTTGGAGTCAGAAGG163410.14969759546691386No Hit
CCCCCCACTGCTAAATTTGACTGGCT162120.148515844667377No Hit
CAAAGAATTCTCCTTTTGGGCTT159470.1460882170559253No Hit
AGCAGCATTGTACAGGGCTATG155150.1421307260063135No Hit
TTAATGCTAATCGTGATAGGGGTT152610.13980386784288432No Hit
TGAGAACTGAATTCCATGGGTT150960.13829232612254647No Hit
TGAGGTAGTAGGTTGTGTGGTT150790.1381365915210571No Hit
ACCGGGTGCTGTAGGCTTT147810.13540665556553783No Hit
AGAATTACTCTGAGACCT146960.13462798255809105No Hit
TTCACAGTGGCTAAGTTCCGC146030.1337760226793552No Hit
AACATTCAACGCTGTCGGTGA143640.1315865773995931No Hit
AGCTCGGTCTGAGGCCCCTCAGT139750.12802300328316024No Hit
TATTGCACTTGTCCCGGCCTGTTT136810.1253297107632855No Hit
CCCCCCACTGCTAAATTTGACTGG134400.12312194376570113No Hit
TGAGGTAGTAGATTGTATAGT131090.12008969946611431No Hit
TAGCTTATCAGACTGATGTTG128250.11748801553535097No Hit
TCGTTTCCCGGGCGGCGCACCA125840.11528024853776661No Hit
ACCCCCCACTGCTAAATTTGACTG124640.11418094546843No Hit
CAAAGTGCTGTTCGTGCAGGT122940.11262359945353644No Hit
CAAAGTGCTGTTCGTGCAGGTAG122600.11231213025055774No Hit
TTCACAGTGGCTAAGTTCTGC120400.11029674129010728No Hit
ACCACAGGGTAGAACCACGGAC118960.10897757760690334No Hit
CTCCCACTGCTTCACTTGACTAGCCT116940.10712708410685337No Hit
ATGCACCTGGGCAAGGATTCTGA115850.10612855048553926No Hit
AACATTCAACGCTGTCGGTGAGA115290.1056155423865155No Hit
TGAATACCGGGTGCTGTAGGCTTT113240.10373756630973213No Hit
TATTGCACTTGTCCCGGCCTGA111450.10209777256463834No Hit
TATTGCACTTGTCCCGGCCTGTAAA110950.10163972961908141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)