FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6285490
Sequences flagged as poor quality0
Sequence length18-26
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG4560597.255742989011199No Hit
CAACGGAATCCCAAAAGCAGCTG3227655.135080956297759No Hit
AAGCTGCCAGTTGAAGAACTGT2718104.3244043026080705No Hit
TTCAAGTAATCCAGGATAGGCT2589424.119678815812292No Hit
TAGCAGCACGTAAATATTGGCG2432543.870088091779639No Hit
TAGCTTATCAGACTGATGTTGAC2200703.5012385669215926No Hit
TAGCTTATCAGACTGATGTTGA1912133.042133548856175No Hit
AACATTCAACGCTGTCGGTGAGT1900323.02334424205591No Hit
TAGCTTATCAGACTGATGTTGACT1806582.8742071023897897No Hit
CCCCCACTGCTAAATTTGACTG1740052.7683601437596748No Hit
AACATTCAACGCTGTCGGTGAG1171701.8641346975335256No Hit
CATAAAGTAGAAAGCACTACT951491.5137881056210416No Hit
TGTAAACATCCTTGACTGGAAGCT901911.4349080183088352No Hit
TGAGGTAGTAGGTTGTATAGTT839431.335504471409548No Hit
TGAGGTAGTAGATTGTATAGTT776201.2349077001156632No Hit
TTCAAGTAATTCAGGATAGGTT630931.003788089711383No Hit
TATTGCACTTGTCCCGGCCTGTAA575660.9158554066588285No Hit
TATTGCACTTGTCCCGGCCTGTAT548670.8729152381119053No Hit
AGCAGCATTGTACAGGGCTATGA475250.7561065247100863No Hit
TATTGCACTTGTCCCGGCCTGT399850.6361476989065292No Hit
GCCTGTCTGAGCGTCGCT377690.6008918954608153No Hit
TTCTCACTACTGCACTTGACTAG366100.5824526011496319No Hit
TGAGGTAGTAGTTTGTACAGTT352950.5615314000976853No Hit
TGTAAACATCCCCGACTGGAAGCT344160.5475468101930001No Hit
TGTCAGTTTGTCAAATACCCCAA341730.5436807631545034No Hit
TGTAAACATCCTACACTCTCAGCT326430.51933898550471No Hit
TATTGCACTTGTCCCGGCCTGTA282690.4497501388117713No Hit
CGCCTGTCTGAGCGTCGCT278280.4427339793715367No Hit
TAGCTTATCAGACTGATGTTGACA268410.4270311463386307No Hit
TTCACAGTGGCTAAGTTCCG264450.42073092153515473No Hit
TGTCAGTTTGTCAAATACCCCA260220.4140011359496236No Hit
CAAAGAATTCTCCTTTTGGGCT211400.33633018269060966No Hit
CAACGGAATCCCAAAAGCAGCTGT209360.33308461233730385No Hit
ACTGGACTTGGAGTCAGAAGGC207950.33084135047546015No Hit
TGTCAGTTTGTCAAATACC207910.33077771184108157No Hit
TTATAAAGCAATGAGACTGATT203440.32366609444927924No Hit
CAACACCAGTCGATGGGCTGT199500.31739768896299253No Hit
TGTAAACATCCTACACTCAGCT192540.3063245665811257No Hit
CTTCTCACTACTGCACTTGACTAG184780.29397867151168805No Hit
CCCATAAAGTAGAAAGCACTAC182280.290001256863029No Hit
CATTGCACTTGTCTCGGTCTGA179840.28611930016593773No Hit
AACCGGGCGGAAACACCA175760.2796281594593262No Hit
TGAGGTAGTAGTTTGTGCTGTT171770.2732802056800663No Hit
AACATTCAACGCTGTCGGTGAGTT153720.24456327191674795No Hit
CCCATAAAGTAGAAAGCACT147470.2346197352951003No Hit
TAGCAGCACGTAAATATTGGC141810.2256148685305362No Hit
TTCAAGTAATCCAGGATAGGC134380.2137939921947215No Hit
CCCCCACTGCTAAATTTGACTGGCTT133130.21180528487039196No Hit
TGATGTAGTAGGTTGTATAGTT126480.20122536190495888No Hit
TTCACAGTGGCTAAGTTCCGC125220.19922074492203473No Hit
TTCAAGTAATCCAGGATAGGCTT123860.1970570313531642No Hit
TGTCAGTTTGTCAAATACCCC123570.19659565125391973No Hit
CCCCCCACTGCTAAATTTGACTGG121750.19370009338969596No Hit
TAGCAGCACGTAAATATTGGCGT120530.19175911504115034No Hit
TAGCTTATCAGACTGATGTTG117510.1869543981455702No Hit
CAAAGAATTCTCCTTTTGGGCTT116410.1852043357001602No Hit
TGATGTAGTAGATTGTATAGTT114990.18294516417972187No Hit
TGTCAGTTTGTCAAATACCC108710.17295389858229032No Hit
CTCCCACTGCTTCACTTGACTAG108420.17249251848304586No Hit
TGTAAACATCCCCGACTGGAAGC108150.1720629577009907No Hit
TCGTACGACTCTTAGCGG102010.16229442732388405No Hit
TTCACAGTGGCTAAGTTCTG101060.16078300975739362No Hit
TAGCAGCACATAATGGTTTGT98800.15718742691500584No Hit
AGCAGCATTGTACAGGGCTATGT95540.15200087821315442No Hit
CCCCCCACTGCTAAATTTGACTGTA93590.14889849478720035No Hit
TACGCCTGTCTGAGCGTCGCT93530.14880303683563254No Hit
TTCACAGTGGCTAAGTTCTGC91460.14550973750654284No Hit
CTACGCCTGTCTGAGCGTCGCT88970.1415482325164784No Hit
AGCTCGGTCTGAGGCCCCTCAGT88110.1401800018773397No Hit
TGAGGTAGTAGGTTGTATAGT87360.138986777482742No Hit
CAACGGAATCCCAAAAGCAGCT84080.1337684094637013No Hit
ATCACATTGCCAGGGATTTCCA82180.13074557433072043No Hit
ACCCCCCACTGCTAAATTTGACTG82110.13063420672055798No Hit
ATCACATTGCCAGGGATTTCC81070.1289796022267158No Hit
CACCCGGCTGTGTGCACATGTGC80930.1287568670063909No Hit
CCCCCCACTGCTAAATTTGACTGGCT80090.12742045568444146No Hit
AGCAGCATTGTACAGGGCTATG79490.12646587616876329No Hit
TGTAAACATCCTTGACTGGAAGC78650.12512946484681386No Hit
TGAGGTAGTAGATTGTATAGT77450.1232203058154575No Hit
TATTGCACTTGTCCCGGCCTGTT76800.12218617800680615No Hit
TGTAGTGTTTCCTACTTTATGGA76460.12164524961458853No Hit
GGAATACCGGGTGCTGTAGGCTTT76400.12154979166302071No Hit
TAGCAGCACATCATGGTTTACA76270.12134296610129044No Hit
TCAGTGCACTACAGAACTTTGT75700.12043611556139618No Hit
AACGGAATCCCAAAAGCAGCTG73960.11766783496592946No Hit
AACATTCAACGCTGTCGGTGA72860.11591777252051949No Hit
ACGCCTGTCTGAGCGTCGCT71940.11445408392981296No Hit
TCGTTTCCCGGCCAATGCACCA71540.1138176975860275No Hit
TGAGGTAGTAAGTTGTATTGTT70420.11203581582342824No Hit
GCGTTCGCGCTTTCCCCTG67940.10809022049195846No Hit
ACTGGACTTGGAGTCAGAAGGCA67630.10759702107552474No Hit
CAAAGTGCTGTTCGTGCAGGT65970.10495601774881513No Hit
TAGCTTATCAGACTGATGTTGACC64750.10301503940026951No Hit
CAGTGCAATAGTATTGTCAAAGC63110.10040585539074917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)