FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18043200
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG15518898.600963243770506No Hit
CAACGGAATCCCAAAAGCAGCTG11217686.217123348408265No Hit
AAGCTGCCAGTTGAAGAACTGT7252784.019675002216902No Hit
AACATTCAACGCTGTCGGTGAGT6945453.849344905559989No Hit
TTCAAGTAATCCAGGATAGGCT6752293.742290724483462No Hit
CCCCCACTGCTAAATTTGACTG6744043.737718364813337No Hit
TAGCAGCACGTAAATATTGGCG5838723.2359670125033255No Hit
AACATTCAACGCTGTCGGTGAG4146062.297851822293163No Hit
TAGCTTATCAGACTGATGTTGAC4140792.2949310543584285No Hit
TAGCTTATCAGACTGATGTTGA4097342.27084996009577No Hit
TAGCTTATCAGACTGATGTTGACT3772852.091009355324998No Hit
CATAAAGTAGAAAGCACTACT2494371.382443247317549No Hit
TGAGGTAGTAGGTTGTATAGTT2162041.1982575152966215No Hit
TATTGCACTTGTCCCGGCCTGTAA1977951.0962301587301586No Hit
TATTGCACTTGTCCCGGCCTGTAT1860391.0310754189944134No Hit
TGTAAACATCCTTGACTGGAAGCT1840321.019952114924182No Hit
TGAGGTAGTAGATTGTATAGTT1571570.8710040347610181No Hit
TATTGCACTTGTCCCGGCCTGT1552310.8603296532765807No Hit
TTCAAGTAATTCAGGATAGGTT1243400.6891238804646627No Hit
AGCAGCATTGTACAGGGCTATGA1238020.6861421477343265No Hit
TATTGCACTTGTCCCGGCCTGTA1073630.5950330318347078No Hit
CCCATAAAGTAGAAAGCACTAC901160.4994457745854394No Hit
CCCATAAAGTAGAAAGCACT888880.4926398864946351No Hit
TGTAAACATCCCCGACTGGAAGCT877290.4862164139398776No Hit
TTCTCACTACTGCACTTGACTAG849460.4707923206526558No Hit
CGCCTGTCTGAGCGTCGCT813490.4508568324909107No Hit
GCCTGTCTGAGCGTCGCT808610.44815221246785497No Hit
TCGTACGACTCTTAGCGG766200.42464751263633943No Hit
TGTAAACATCCTACACTCTCAGCT759850.4211281812538796No Hit
TGAGGTAGTAGTTTGTACAGTT722880.4006384676775738No Hit
CAACGGAATCCCAAAAGCAGCTGT698070.3868881351423251No Hit
ACTGGACTTGGAGTCAGAAGGC668150.37030571073867163No Hit
AACCGGGCGGAAACACCA654620.3628070408796666No Hit
TGTCAGTTTGTCAAATACCCCAA653690.3622916112441252No Hit
CAACACCAGTCGATGGGCTGT626890.3474383701339009No Hit
CATTGCACTTGTCTCGGTCTGA623320.3454597854039195No Hit
TTAATGCTAATCGTGATAGGGGTT622010.34473375011084506No Hit
TTCACAGTGGCTAAGTTCCG535570.29682650527622595No Hit
AACATTCAACGCTGTCGGTGAGTT517600.2868670745765718No Hit
TAGCTTATCAGACTGATGTTGACA512120.28382991930477963No Hit
TGAGGTAGTAGTTTGTGCTGTT476790.26424913540835326No Hit
CTTCTCACTACTGCACTTGACTAG454430.25185665513877803No Hit
TGTCAGTTTGTCAAATACCCCA430310.23848873813957613No Hit
CAAAGAATTCTCCTTTTGGGCT380330.2107885519198368No Hit
CCCCCCACTGCTAAATTTGACTGG371390.20583377671366498No Hit
CTCCCACTGCTTCACTTGACTAG365860.2027689101711448No Hit
CCCCCACTGCTAAATTTGACTGGCTT358290.19857342378292098No Hit
CAACGGAATCCCAAAAGCAGCT354530.1964895362241731No Hit
CCCCCCACTGCTAAATTTGACTGTA351720.1949321628092578No Hit
TTATAAAGCAATGAGACTGATT343970.19063691584641304No Hit
TGTAAACATCCTACACTCAGCT339430.18812073246430788No Hit
TTCAAGTAATCCAGGATAGGC318900.17674248470337856No Hit
TACGCCTGTCTGAGCGTCGCT316720.17553427329963645No Hit
TAGCAGCACGTAAATATTGGC315130.17465305489048508No Hit
CACCCGGCTGTGTGCACATGTGC311640.17271880819366853No Hit
TTCAAGTAATCCAGGATAGGCTT310160.17189855458011882No Hit
CTACGCCTGTCTGAGCGTCGCT296790.16448856078744345No Hit
TATTGCACTTGTCCCGGCCTGTT294440.16318613106322605No Hit
AACGGAATCCCAAAAGCAGCTG289140.1602487363660548No Hit
AGCTCGGTCTGAGGCCCCTCAGT277470.15378092577813246No Hit
TTCACAGTGGCTAAGTTCTG273950.15183005231887914No Hit
TCCTGTCTGAGCGTCGCT268410.14875964352221335No Hit
ATCCCGGACGAGCCCCCA259240.14367739647069255No Hit
AGCAGCATTGTACAGGGCTATGT259080.14358872040436285No Hit
TGTAAACATCCCCGACTGGAAGC254210.1408896426354527No Hit
TGTCAGTTTGTCAAATACC254010.14077879755254055No Hit
TGAGAACTGAATTCCATAGGCTGT251000.13911057905471313No Hit
CCCATAAAGTAGAAAGCACTA250970.1390939522922763No Hit
TCAGTGCACTACAGAACTTTGT247280.13704886051254767No Hit
TTCACAGTGGCTAAGTTCCGC246350.13653343087700628No Hit
CAAAGAATTCTCCTTTTGGGCTT244120.13529750820253614No Hit
AACATTCAACGCTGTCGGTGA235470.13050345836658686No Hit
CACTAGATTGTGAGCTCCTGGA232720.12897933847654516No Hit
CCCCCCACTGCTAAATTTGACTGGCT231280.1281812538795779No Hit
TAGCAGCACGTAAATATTGGCGT227830.1262691761993438No Hit
CCCCCCACTGCTAAATTTGACTGT226010.12526048594484349No Hit
CCCCCACTGCTAAATTTGACTGTA224970.12468409151370045No Hit
TGATGTAGTAGGTTGTATAGTT224700.12453445065176909No Hit
AGCAGCATTGTACAGGGCTATG222810.12348696461824953No Hit
TATTGCACTTGTCCCGGCCTGTAG219570.12169127427507315No Hit
TGAGGTAGTAGGTTGTATAGT219350.12156934468386983No Hit
TGAGGTAGTAGGTTGTGTGGTT217750.12068258402057284No Hit
TAGCTTATCAGACTGATGTTG217520.12055511217522391No Hit
ACCCCCCACTGCTAAATTTGACTG217020.1202779994679436No Hit
AACATTCAACGCTGTCGGTGAGA216100.11976811208654783No Hit
ACTGGACTTGGAGTCAGAAGG209290.11599383701339008No Hit
ACGCCTGTCTGAGCGTCGCT207510.1150073157754722No Hit
TCGTTTCCCGGCCAATGCACCA203260.11265185776358962No Hit
ACTGGACTTGGAGTCAGAAGGCA202890.11244679436020219No Hit
TTCACAGTGGCTAAGTTCTGC200280.11100026602819899No Hit
CAAAGTGCTGTTCGTGCAGGT198880.11022435044781413No Hit
CACTAGATTGTGAGCTCCTGGAA198010.1097421743371464No Hit
ACCACAGGGTAGAACCACGGAC196580.10894963199432474No Hit
TGTCAGTTTGTCAAATACCCC193050.10699321628092578No Hit
ACTGGACTTGGAGTCAGAAGGCT183440.1016671100469983No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)