FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14104835
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG179698312.740191572606129No Hit
CCCCCCACTGCTAAATTTGACTG9845226.980032024479549No Hit
CCCCCACTGCTAAATTTGACTG5315713.7687147705024557No Hit
TTCAAGTAATCCAGGATAGGCT5225663.704871414660292No Hit
TAGCAGCACGTAAATATTGGCG4542453.220491413050915No Hit
AACATTCAACGCTGTCGGTGAG3462482.4548177982939894No Hit
AAGCTGCCAGTTGAAGAACTGT3086002.187902233524887No Hit
TAGCTTATCAGACTGATGTTGAC2115721.4999962778720914No Hit
TGAGGTAGTAGATTGTATAGTT2100831.4894396141464965No Hit
TAGCTTATCAGACTGATGTTGA2084821.4780888964670624No Hit
TGAGGTAGTAGGTTGTATAGTT1633201.1579008191162818No Hit
CATAAAGTAGAAAGCACTACT1545491.0957164688562469No Hit
TATTGCACTTGTCCCGGCCTGT1458351.0339362353405763No Hit
TAGCTTATCAGACTGATGTTGACT1312700.9306737725042513No Hit
TGAGAACTGAATTCCATGGGTT1251800.8874970887642429No Hit
GCCTGTCTGAGCGTCGCT1210350.8581100027047462No Hit
TGAGAACTGAATTCCATAGGCTGT1181270.8374929589747062No Hit
AACATTCAACGCTGTCGGTGAGT1142650.8101122771021426No Hit
CCCATAAAGTAGAAAGCACTAC1101860.7811931156940155No Hit
TATTGCACTTGTCCCGGCCTGTAA1010640.716520257060788No Hit
TTAATGCTAATCGTGATAGGGGTT956660.678249692392715No Hit
TTCTCACTACTGCACTTGACTAG916630.6498693533104074No Hit
TATTGCACTTGTCCCGGCCTGTAT893850.6337188630707129No Hit
TGAGGTAGTAGTTTGTACAGTT858280.6085005602688723No Hit
TTCAAGTAATTCAGGATAGGTT847980.6011980998005294No Hit
CAACGGAATCCCAAAAGCAGCTGT821900.5827079863039872No Hit
TGTAAACATCCTTGACTGGAAGCT722040.5119095685982856No Hit
TTCACAGTGGCTAAGTTCCGC674350.47809846765311326No Hit
TATTGCACTTGTCCCGGCCTGTA666360.47243374346456374No Hit
AGCAGCATTGTACAGGGCTATGA653780.4635148160187624No Hit
CCCATAAAGTAGAAAGCACT640270.45393654020057667No Hit
CCCCCACTGCTAAATTTGACTGGCTT529100.37511959551458773No Hit
CATTGCACTTGTCTCGGTCTGA515310.3653428062079422No Hit
TCGTACGACTCTTAGCGG514830.3650024973705825No Hit
CAACGGAATCCCAAAAGCAGCT493680.35000763922442196No Hit
AACGGAATCCCAAAAGCAGCTG476490.33782032898647874No Hit
AACCGGGCGGAAACACCA426610.3024565689708529No Hit
CACCCGGCTGTGTGCACATGTGC426110.3021020805986032No Hit
TAGCAGCACGTAAATATTGGC425340.3015561685053388No Hit
CCCATAAAGTAGAAAGCACTA412320.2923252912919577No Hit
CAACACCAGTCGATGGGCTGT388960.2757635945404537No Hit
CTCCCACTGCTTCACTTGACTAG374680.2656394066290034No Hit
TCGTTTCCCGGCCAATGCACCA369770.2621583308135118No Hit
TGGCTCAGTTCAGCAGGAAC360050.25526707685697847No Hit
TGTAAACATCCCCGACTGGAAGCT353350.25051693266883307No Hit
TGTAAACATCCCCGACTGGA338160.2397475759198885No Hit
TGTAAACATCCTACACTCTCAGCT335070.23755683777938558No Hit
CTTCTCACTACTGCACTTGACTAG314490.22296609637758968No Hit
TTCACAGTGGCTAAGTTCCG313120.2219947982376256No Hit
TAGCTTATCAGACTGATGTTGACA312750.22173247684216085No Hit
ACTGGACTTGGAGTCAGAAGGC307120.21774093777062972No Hit
CCCCCCACTGCTAAATTTGACTGG289140.204993535904532No Hit
CAAAGAATTCTCCTTTTGGGCT278560.19749256194772927No Hit
TGAGGTAGTAGATTGTATAGT276840.19627312194719043No Hit
TTCACAGTGGCTAAGTTCTGC251330.17818712519501292No Hit
TGAGGTAGTAGTTTGTGCTGTT250310.1774639689156236No Hit
TTCAAGTAATCCAGGATAGGC240930.17081376705222004No Hit
ATCACATTGCCAGGGATTTCC237470.16836070751625243No Hit
AAACGGAATCCCAAAAGCAGCTG237040.1680558475161177No Hit
CCCCCCACTGCTAAATTTTACTG235720.1671199982133786No Hit
ACCGGGTGCTGTAGGCTT235510.16697111309703375No Hit
GGAATACCGGGTGCTGTAGGCTTT232570.16488672146820577No Hit
ACCCCCCACTGCTAAATTTGACTG231840.16436916844472124No Hit
TGATGTAGTAGATTGTATAGTT217470.1541811726262661No Hit
TGTCAGTTTGTCAAATACCCCA217070.15389758192846636No Hit
TTAATGCTAATCGTGATAGGGGTTT210700.14938140006600573No Hit
CAACGGAATCCAAAAAGCAGCTG209150.14828248611203182No Hit
TCCCACTGCTTCACTTGACTAG205720.14585069587839916No Hit
GGAATACCGGGTGCTGTAGGCTT198940.1410438335506938No Hit
TATTGCACTTGTCCCGGCCTGTT197890.1402994079689695No Hit
TTCACAGTGGCTAAGTTCTG194900.13817956750291654No Hit
TGAGGTAGTAAGTTGTATTGTT191790.1359746498275237No Hit
AGCTCGGTCTGAGGCCCCTCAGT188740.13381227075680077No Hit
CAACGTAATCCCAAAAGCAGCTG186540.13225252191890227No Hit
CCCCCCACTGCTAAATTTGACTGTA178560.12659488749779774No Hit
CAACTGAATCCCAAAAGCAGCTG177290.1256944870322836No Hit
ACCGGGTGCTGTAGGCTTT172070.12199362842599719No Hit
TAGCAGCACATCATGGTTTACA171660.12170294796075246No Hit
TGATGTAGTAGGTTGTATAGTT166540.11807298702891597No Hit
AGCTACATTGTCTGCTGGGTTT156790.11116046377004764No Hit
TGAGAACTGAATTCCATGGGTTG153400.10875703260619497No Hit
CCCCCCACTGCTAAATTTGACTGGCT153250.10865068609452007No Hit
ACCCCCACTGCTAAATTTGACTG142410.1009653781841475No Hit
AGAGGTAGTAGGTTGCATAGTT141720.10047618423044297No Hit
TCAGGCTCAGTCCCCTCCCGAT141430.10027058097453817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)