FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10346621
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG123127411.900252265933004No Hit
CAACGGAATCCCAAAAGCAGCTG7782807.522069282328984No Hit
CCCCCACTGCTAAATTTGACTG5171294.998047188545903No Hit
TTCAAGTAATCCAGGATAGGCT3387573.2740833939892062No Hit
AACATTCAACGCTGTCGGTGAGT2886362.7896643744851577No Hit
TAGCAGCACGTAAATATTGGCG2738052.646322891309153No Hit
AAGCTGCCAGTTGAAGAACTGT2285932.209349313171904No Hit
AACATTCAACGCTGTCGGTGAG2232512.1577189306537856No Hit
TAGCTTATCAGACTGATGTTGA1557441.505264375683617No Hit
TAGCTTATCAGACTGATGTTGAC1349941.3047158101181053No Hit
CATAAAGTAGAAAGCACTACT1318551.2743774030188213No Hit
TAGCTTATCAGACTGATGTTGACT1039091.0042795614143012No Hit
TCGTACGACTCTTAGCGG841900.8136956016848398No Hit
TATTGCACTTGTCCCGGCCTGT748800.7237145344359284No Hit
TATTGCACTTGTCCCGGCCTGTAA741380.7165431110311279No Hit
TATTGCACTTGTCCCGGCCTGTAT734530.7099225921196881No Hit
CCCATAAAGTAGAAAGCACT708670.684928925105114No Hit
ATCCCGGACGAGCCCCCA632640.6114459976836882No Hit
TGAGGTAGTAGGTTGTATAGTT624660.6037333347766387No Hit
AGCAGCATTGTACAGGGCTATGA622400.6015490467854191No Hit
TATTGCACTTGTCCCGGCCTGTA556600.5379534052711509No Hit
CCCATAAAGTAGAAAGCACTAC549000.5306080120263418No Hit
TTCAAGTAATTCAGGATAGGTT539100.5210396708258667No Hit
TGAGGTAGTAGATTGTATAGTT501460.4846606442818385No Hit
TTAATGCTAATCGTGATAGGGGTT444420.42953153498132385No Hit
CAACACCAGTCGATGGGCTGT443440.42858436585238796No Hit
CATTGCACTTGTCTCGGTCTGA443240.4283910660301561No Hit
TGTAAACATCCTTGACTGGAAGCT431730.417266661260715No Hit
ACTGGACTTGGAGTCAGAAGGC403370.3898567464682431No Hit
TTCTCACTACTGCACTTGACTAG395390.38214408356119356No Hit
CAACGGAATCCCAAAAGCAGCTGT394160.38095528965446784No Hit
CAACGGAATCCCAAAAGCAGCT390610.3775242178098531No Hit
TTCACAGTGGCTAAGTTCCG376890.36426385000475037No Hit
CGCCTGTCTGAGCGTCGCT370900.35847452032890736No Hit
TGTAAACATCCTACACTCTCAGCT328510.31750462300687343No Hit
CTCCCACTGCTTCACTTGACTAG277510.26821316833775977No Hit
ACCGGGTGCTGTAGGCTTA275790.2665507898665661No Hit
CAAAGAATTCTCCTTTTGGGCT270760.26168929933743584No Hit
TGTAAACATCCCCGACTGGAAGCT264710.25584197971492334No Hit
CCCATAAAGTAGAAAGCACTA254530.2460030187633238No Hit
ACCGGGTGCTGTAGGCTT250420.24203070741645993No Hit
CCCCCCACTGCTAAATTTGACTGTA250130.24175042267422378No Hit
CCCCCCACTGCTAAATTTGACTGG248580.24025234905192722No Hit
CCCCCCACTGCTAAATTTGACTGT246210.23796174615848012No Hit
CTTCTCACTACTGCACTTGACTAG242150.23403775976717422No Hit
GCCTGTCTGAGCGTCGCT238770.23077099277145652No Hit
CACCCGGCTGTGTGCACATGTGC230650.22292301998884465No Hit
CCCCCCACTGCTAAATTTGACTGA225240.21769425979747398No Hit
AACATTCAACGCTGTCGGTGAGTT224150.21664077576631058No Hit
CACTAGATTGTGAGCTCCTGGA211600.20451121192126398No Hit
AGCTCGGTCTGAGGCCCCTCAGT208950.20194998927669233No Hit
ACCGGGTGCTGTAGGCTTT204220.19737844848090985No Hit
AACCGGGCGGAAACACCA195230.18868962147158963No Hit
AACGGAATCCCAAAAGCAGCTG189020.18268766199129163No Hit
CACTAGATTGTGAGCTCCTGGAA187230.18095762858231687No Hit
TGTCAGTTTGTCAAATACCCCAA185550.1793339100755696No Hit
TCAGTGCACTACAGAACTTTGT183770.1776135416577064No Hit
CTACGCCTGTCTGAGCGTCGCT178930.1729356859596964No Hit
TTCAAGTAATCCAGGATAGGC173730.16790989058166914No Hit
AGCTACATTGTCTGCTGGGTTT173670.16785190063499958No Hit
ACTGGACTTGGAGTCAGAAGG173270.16746530099053594No Hit
TTCACAGTGGCTAAGTTCTG169460.1637829393770198No Hit
TAGCAGCACGTAAATATTGGC168120.16248783056806662No Hit
CCCCCACTGCTAAATTTGACTGGCTT167820.1621978808347189No Hit
GGAATACCGGGTGCTGTAGGCTTT167000.16140535156356844No Hit
TGAGGTAGTAGTTTGTACAGTT165290.1597526380834864No Hit
TGTCAGTTTGTCAAATACC165260.1597236431101516No Hit
ACCCCCCACTGCTAAATTTGACTG162610.15716242046558002No Hit
TGTCAGTTTGTCAAATACCCCA161390.15598329154996593No Hit
CCCCCACTGCTAAATTTGACTGTA157670.1523879148564541No Hit
ACTGGACTTGGAGTCAGAAGGCA152860.14773905413177887No Hit
TCGTTTCCCGGCCAATGCACCA149060.14406635750937433No Hit
TGTAAACATCCTACACTCAGCT147030.14210436431372134No Hit
TACGCCTGTCTGAGCGTCGCT144710.13986208637583225No Hit
TATTGCACTTGTCCCGGCCTGTT143080.13828669282464293No Hit
TGAGGTAGTAGTTTGTGCTGTT142540.13776478330461703No Hit
TAGCTTATCAGACTGATGTTGACA140830.136112069824535No Hit
TCGTTTCCCGGGCGGCGCACCA137520.1329129577665984No Hit
TGAGAACTGAATTCCATAGGCTGT134110.12961719579754588No Hit
GGAATACCGGGTGCTGTAGGCTT132990.1285347167930477No Hit
AGCAGCATTGTACAGGGCTATG132690.12824476705969998No Hit
CCCCCACTGCTAAATTTGACTGT125310.12111200361934588No Hit
TTCAAGTAATCCAGGATAGGCTT124290.12012617452596361No Hit
AGCAGCATTGTACAGGGCTATGT123980.11982655980150429No Hit
ACTGGACTTGGAGTCAGAAGGCT118530.11455913964568722No Hit
ATACCGGGTGCTGTAGGCTTA114980.11112806780107244No Hit
AGCTACATCTGGCTACTGGGTCTC110420.10672083185418697No Hit
ATCCCACCGCTGCCACCA110390.10669183688085222No Hit
CCCCCACTGCTAAATTTGACTGA110300.10660485196084789No Hit
CAAAGAATTCTCCTTTTGGGCTT110080.10639222215639291No Hit
ACGCCTGTCTGAGCGTCGCT107370.10377300956515174No Hit
AATGACACGATCACTCCCGTTG106620.10304813523178244No Hit
CCCCCCACTGCTAAATTTGACTGGCT106090.10253589070286812No Hit
CCCCCACTGCTAAATTTGACTGG105500.10196565622728425No Hit
AACATTCAACGCTGTCGGTGA104950.10143408171614675No Hit
ACCACAGGGTAGAACCACGGAC104880.10136642677836562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)