FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10174429
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9977579.806515923399731No Hit
CCCCCCACTGCTAAATTTGACTG4721394.640447144503145No Hit
TAGCAGCACGTAAATATTGGCG3448193.389074708762526No Hit
AAGCTGCCAGTTGAAGAACTGT3179693.1251778355325888No Hit
TTCAAGTAATCCAGGATAGGCT3084963.032071873517423No Hit
AACATTCAACGCTGTCGGTGAGT3009532.9579350349783757No Hit
TAGCTTATCAGACTGATGTTGACT2255052.2163897354829447No Hit
TAGCTTATCAGACTGATGTTGAC2244322.2058436891151336No Hit
GGAATACCGGGTGCTGTAGGCTTT2231242.192987930821474No Hit
AACATTCAACGCTGTCGGTGAG2015771.9812119186246226No Hit
GCCTGTCTGAGCGTCGCT1929241.8961653769464606No Hit
TATTGCACTTGTCCCGGCCTGTAT1859581.8276996183274756No Hit
TATTGCACTTGTCCCGGCCTGTAA1750151.720145671074023No Hit
TAGCTTATCAGACTGATGTTGA1731751.7020611181226977No Hit
GGAATACCGGGTGCTGTAGGCTT1343591.320555679340826No Hit
CCCCCACTGCTAAATTTGACTG1295781.27356532735154No Hit
TATTGCACTTGTCCCGGCCTGT1013110.9957413826368046No Hit
TCGTACGACTCTTAGCGG938260.9221746006581794No Hit
TGAGGTAGTAGGTTGTATAGTT894100.8787716735749986No Hit
TGTAAACATCCTTGACTGGAAGCT866800.8519397009896084No Hit
TGAATACCGGGTGCTGTAGGCTTT748100.7352746773307869No Hit
TATTGCACTTGTCCCGGCCTGTA735010.7224090904757406No Hit
TCCTGTCTGAGCGTCGCT670810.6593097263738339No Hit
TTCAAGTAATTCAGGATAGGTT661550.6502084785298516No Hit
CATAAAGTAGAAAGCACTACT658900.6476039097624053No Hit
AGCAGCATTGTACAGGGCTATGA652750.6415593445096526No Hit
CAACGGAATCCCAAAAGCAGCTGT611780.601291728508794No Hit
TGAGGTAGTAGATTGTATAGTT610130.5996700158800067No Hit
TGTAAACATCCCCGACTGGAAGCT594830.5846323169585241No Hit
CCCATAAAGTAGAAAGCACTAC524920.5159208443048745No Hit
ACTGGACTTGGAGTCAGAAGGC481860.4735990589742186No Hit
CAACGGAATCCCAAAAGCAGCT450830.4431010329916303No Hit
CACCCGGCTGTGTGCACATGTGC440250.4327024150446182No Hit
TGAATACCGGGTGCTGTAGGCTT438780.43125761652078953No Hit
CATTGCACTTGTCTCGGTCTGA363620.3573861491391802No Hit
AACCGGGCGGAAACACCA358270.35212786879735464No Hit
CCCATAAAGTAGAAAGCACT342310.3364414848243572No Hit
CAACACCAGTCGATGGGCTGT325700.3201162443612315No Hit
GGAATACCGGGTGCTGTAGGCTTA309500.3041939749149559No Hit
TAGCTTATCAGACTGATGTTGACA303140.29794300987308475No Hit
CTCCCACTGCTTCACTTGACTAG302130.2969503251730392No Hit
TGAGGTAGTAGTTTGTACAGTT293670.28863536223998415No Hit
TGTAAACATCCTACACTCTCAGCT282980.27812863011771966No Hit
CTTCTCACTACTGCACTTGACTAG276430.27169092240950327No Hit
TTCTCACTACTGCACTTGACTAG272740.26806418325785164No Hit
CCCCCACTGCTAAATTTGACTGGCTT272050.2673860125221769No Hit
CAAAGAATTCTCCTTTTGGGCT246700.24247060940717166No Hit
CACTAGATTGTGAGCTCCTGGA221520.2177222918357384No Hit
TAGCAGCACGTAAATATTGGC214050.2103803564799558No Hit
TATTGCACTTGTCCCGGCCTGTAG210300.20669464595998457No Hit
CACTAGATTGTGAGCTCCTGGAA207810.2042473341747237No Hit
TATTGCACTTGTCCCGGCCTGTT204900.20138722281122606No Hit
AACATTCAACGCTGTCGGTGAGTT204050.2005517950933659No Hit
AACGGAATCCCAAAAGCAGCTG202150.19868436842991385No Hit
TCGTTTCCCGGCCAATGCACCA200910.1974656268179767No Hit
CCCCCCACTGCTAAATTTGACTGGCT193080.18976986325227685No Hit
GGAATACCGGGTGCTGTAGGCT184870.18170061435388657No Hit
TACGCCTGTCTGAGCGTCGCT181930.17881101730622917No Hit
TGTAAACATCCCCGACTGGAAGC181020.1779166182200495No Hit
CAAAGAATTCTCCTTTTGGGCTT179540.1764619911348342No Hit
CTCCCACTGCTTCACTTGACTAGCCT179360.1762850770298756No Hit
TTCAAGTAATCCAGGATAGGCTT178680.1756167348555875No Hit
AGCTCGGTCTGAGGCCCCTCAGT175920.17290405191288868No Hit
TGAGGTAGTAGTTTGTGCTGTT171210.16827479949980487No Hit
TATTGCACTTGTCCCGGCCTGTTT169850.16693811515122864No Hit
CGCCTGTCTGAGCGTCGCT166840.16397971817386509No Hit
TATTGCACTTGTCCCGGCCTGTAAT157660.1549570988209756No Hit
CTACGCCTGTCTGAGCGTCGCT153780.15114361700297874No Hit
AGCAGCATTGTACAGGGCTATGT151890.14928601890091325No Hit
TGTCAGTTTGTCAAATACCCCAA150830.14824419139393474No Hit
TTCACAGTGGCTAAGTTCCGC146270.14376236740164977No Hit
ACTGGACTTGGAGTCAGAAGGCA143190.1407351704945801No Hit
TCGTTTCCCGGGCGGCGCACCA140770.13835665863902535No Hit
TGATGTAGTAGGTTGTATAGTT140120.137717802148897No Hit
ATCCCGGACGAGCCCCCA138650.1362730036250683No Hit
TTCAAGTAATCCAGGATAGGC138200.13583071836267174No Hit
ACTGGACTTGGAGTCAGAAGGCT135190.13287232138530822No Hit
CCCCCCACTGCTAAATTTGACTGG134760.13244969324568484No Hit
TGTCAGTTTGTCAAATACCCCA124230.12210021810560572No Hit
CCCATAAAGTAGAAAGCACTA121830.11974136337282416No Hit
ACCCCCCACTGCTAAATTTGACTG121710.11962342063618508No Hit
CGTTCGCGCTTTCCCCTG118040.11601633860730662No Hit
TATTGCACTTGTCCCGGCCTGTAAA114800.11283188471805151No Hit
TTAATACCGGGTGCTGTAGGCTTT114160.11220285678930975No Hit
TCAGTGCACTACAGAACTTTGT113130.11119051496649099No Hit
TAGCAGCACGTAAATATTGGCGT112960.11102342942291897No Hit
TTATAAAGCAATGAGACTGATT112780.11084651531796035No Hit
TTCACAGTGGCTAAGTTCTGC111790.10987348774068795No Hit
ACTTGACTTGGAGTCAGAAGGC107490.10564720634445432No Hit
CAACGGAATCCCAAAAGCAGCTGA107110.1052737210117639No Hit
TGAATACCGGGTGCTGTAGGCTTA106940.10510663546819188No Hit
ATGCACCTGGGCAAGGATTCTGA106440.10461520739886239No Hit
ACCACAGGGTAGAACCACGGAC103040.10127349652742183No Hit
TATTGCACTTGTCCCGGCCTGA102380.10062481147590689No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)