FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7594409
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG78949010.395673975420602No Hit
AACATTCAACGCTGTCGGTGAGT3278004.316333239360693No Hit
AAGCTGCCAGTTGAAGAACTGT3127934.1187273427069835No Hit
TAGCAGCACGTAAATATTGGCG2771483.6493688975666174No Hit
CCCCCCACTGCTAAATTTGACTG2396723.1559006105675897No Hit
TTCAAGTAATCCAGGATAGGCT2310053.0417771810815037No Hit
AACATTCAACGCTGTCGGTGAG1949412.566901519262394No Hit
TAGCTTATCAGACTGATGTTGAC1635762.15390032325096No Hit
TAGCTTATCAGACTGATGTTGACT1524072.0068316046712784No Hit
TAGCTTATCAGACTGATGTTGA1427131.879185068910563No Hit
TATTGCACTTGTCCCGGCCTGTAA1136041.4958899369259675No Hit
TATTGCACTTGTCCCGGCCTGTAT1047791.3796860295514766No Hit
TATTGCACTTGTCCCGGCCTGT871821.1479760966258203No Hit
CCCCCACTGCTAAATTTGACTG833871.0980051245593963No Hit
TGAGGTAGTAGGTTGTATAGTT818171.0773320214910733No Hit
CGCCTGTCTGAGCGTCGCT748280.9853037938831053No Hit
GCCTGTCTGAGCGTCGCT662360.8721679330149325No Hit
CATAAAGTAGAAAGCACTACT651010.8572227279305079No Hit
TGTAAACATCCTTGACTGGAAGCT639890.8425803772222434No Hit
AGCAGCATTGTACAGGGCTATGA595600.784261158439057No Hit
TATTGCACTTGTCCCGGCCTGTA500540.6590901280139113No Hit
GGAATACCGGGTGCTGTAGGCTTT500380.6588794467087564No Hit
TTAATGCTAATCGTGATAGGGGTT446990.588577728694886No Hit
ACTGGACTTGGAGTCAGAAGGC439310.5784650260474515No Hit
CAACGGAATCCCAAAAGCAGCTGT429830.5659821587170246No Hit
TGTAAACATCCCCGACTGGAAGCT427120.5624137441109637No Hit
CCCATAAAGTAGAAAGCACTAC393860.5186183678018922No Hit
TGAGGTAGTAGATTGTATAGTT392650.5170250904316583No Hit
CCCATAAAGTAGAAAGCACT371920.4897286938325286No Hit
TTCAAGTAATTCAGGATAGGTT365360.4810907603211784No Hit
CACCCGGCTGTGTGCACATGTGC350580.461629074757496No Hit
CAACACCAGTCGATGGGCTGT339450.44697355646765924No Hit
GGAATACCGGGTGCTGTAGGCTT332180.43740072466468427No Hit
CATTGCACTTGTCTCGGTCTGA311390.41002532257612145No Hit
TCGTACGACTCTTAGCGG296030.3897999172812526No Hit
CAACGGAATCCCAAAAGCAGCT287050.3779754290294347No Hit
TCCTGTCTGAGCGTCGCT248540.32726707239496844No Hit
CTACGCCTGTCTGAGCGTCGCT228010.3002340274272824No Hit
TGAGGTAGTAGTTTGTACAGTT226390.29810087921258915No Hit
TGTCAGTTTGTCAAATACCCCAA226350.2980482088863004No Hit
TGTAAACATCCTACACTCTCAGCT212070.279244902401227No Hit
AACATTCAACGCTGTCGGTGAGTT208740.274860097737691No Hit
TACGCCTGTCTGAGCGTCGCT198820.26179785681808815No Hit
TGAATACCGGGTGCTGTAGGCTTT196120.2582426097935995No Hit
TAGCTTATCAGACTGATGTTGACA191510.2521723546888244No Hit
CACTAGATTGTGAGCTCCTGGA190100.25031572568714694No Hit
AACCGGGCGGAAACACCA188760.2485512697564748No Hit
TCGTTTCCCGGCCAATGCACCA184640.24312622614873652No Hit
ACGCCTGTCTGAGCGTCGCT180820.23809620998816367No Hit
AACGGAATCCCAAAAGCAGCTG173360.22827319413531716No Hit
TGATGTAGTAGGTTGTATAGTT156350.20587513788103856No Hit
TGAGAACTGAATTCCATGGGTT154780.20380782757420624No Hit
CACTAGATTGTGAGCTCCTGGAA150190.1977639076325755No Hit
TGAGGTAGTAGTTTGTGCTGTT148730.19584144072303716No Hit
TATTGCACTTGTCCCGGCCTGTT145840.19203600964867706No Hit
GCGTTCGCGCTTTCCCCTG138410.18225249654054712No Hit
TTCAAGTAATCCAGGATAGGCTT136240.179395131339384No Hit
TAGCAGCACGTAAATATTGGC136060.17915811487108477No Hit
TTCTCACTACTGCACTTGACTAG133040.17518150523628634No Hit
TGTAAACATCCCCGACTGGAAGC132610.17461529922868257No Hit
ATCCCGGACGAGCCCCCA132180.17404909322107884No Hit
TGAATACCGGGTGCTGTAGGCTT128880.16970379130225932No Hit
TGTCAGTTTGTCAAATACCCCA126680.16680692335637967No Hit
TATTGCACTTGTCCCGGCCTGTAG126620.1667279178669466No Hit
CAAAGAATTCTCCTTTTGGGCT122380.16114486328034217No Hit
AGCAGCATTGTACAGGGCTATGT122260.160986852301476No Hit
ACCACAGGGTAGAACCACGGAC121650.160183629825573No Hit
ACTGGACTTGGAGTCAGAAGGCA121570.16007828917299555No Hit
CCCCCACTGCTAAATTTGACTGGCTT121000.15932773702338127No Hit
ACTGGACTTGGAGTCAGAAGGCT118270.15573298725417606No Hit
CACCCGTAGAACCGACCTTGCG113490.1494388832626739No Hit
TTCAAGTAATCCAGGATAGGC111830.14725306472169197No Hit
CTCCCACTGCTTCACTTGACTAG110680.14573879284089122No Hit
ACCGGGTGCTGTAGGCTT110610.14564661976988597No Hit
CTTCTCACTACTGCACTTGACTAG110040.1448960676202717No Hit
CAAAGAATTCTCCTTTTGGGCTT108380.14271024907928978No Hit
TTATAAAGCAATGAGACTGATT105590.1390364938206515No Hit
TGAGGTAGTAGGTTGTGTGGTT104220.1372325351452628No Hit
AGCTCGGTCTGAGGCCCCTCAGT98190.1292924834572381No Hit
AGCAGCATTGTACAGGGCTATG97510.1283970879103298No Hit
AACATTCAACGCTGTCGGTGA95470.12571090126960505No Hit
CCCATAAAGTAGAAAGCACTA94990.1250788573541404No Hit
ACTTGACTTGGAGTCAGAAGGC92740.12211615150039984No Hit
TTCACAGTGGCTAAGTTCCGC92490.12178696196109534No Hit
ACCGGGTGCTGTAGGCTTT92320.12156311307436826No Hit
TTCACAGTGGCTAAGTTCTGC90400.11903493741250964No Hit
ATCCCACCGCTGCCACCA89600.11798153088673523No Hit
ACTGGACTTGGAGTCAGAAGG88500.11653309691379539No Hit
TCGTTTCCCGGGCGGCGCACCA84140.11079203134832478No Hit
TATTGCACTTGTCCCGGCCTGTAAT83960.11055501488002556No Hit
TTCACAGTGGCTAAGTTCCG83690.11019949017757669No Hit
CAACGGAATCCCAAAAGCAGCTGA82900.10915925123337444No Hit
CAAAGTGCTGTTCGTGCAGGTAG82890.10914608365180227No Hit
CAAAGTGCTGTTCGTGCAGGT82450.10856671006262635No Hit
TCAGTGCACTACAGAACTTTGT81200.10692076236610379No Hit
TAGCAGCACGTAAATATTGGCGT79090.10414240265437377No Hit
CCAAGTCCTTCTGATCGAGGCCC78900.10389221860450235No Hit
AACATTCAACGCTGTCGGTGAGA78730.10366836971777527No Hit
TATTGCACTTGTCCCGGCCTGTTT77140.1015747242477986No Hit
CCCCCCACTGCTAAATTTGACTGGCT76680.1009690154954783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)