FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17142411
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG16061749.36959217696974No Hit
CCCCCCACTGCTAAATTTGACTG6861564.0026808364354345No Hit
AACATTCAACGCTGTCGGTGAGT6683643.898891468650471No Hit
AAGCTGCCAGTTGAAGAACTGT6215663.625896030610863No Hit
TAGCAGCACGTAAATATTGGCG5831363.4017151963046506No Hit
TTCAAGTAATCCAGGATAGGCT5519403.219733793571978No Hit
TAGCTTATCAGACTGATGTTGAC4498582.624239962511691No Hit
TAGCTTATCAGACTGATGTTGACT4450342.5960992301491315No Hit
AACATTCAACGCTGTCGGTGAG4416072.5761078765408203No Hit
TAGCTTATCAGACTGATGTTGA3521412.0542092941302132No Hit
TATTGCACTTGTCCCGGCCTGTAA3305481.9282468492909193No Hit
TATTGCACTTGTCCCGGCCTGTAT3211171.8732312508433033No Hit
TGAGGTAGTAGGTTGTATAGTT2008741.1717954959777828No Hit
CCCCCACTGCTAAATTTGACTG1947411.1360187315541554No Hit
TGTAAACATCCTTGACTGGAAGCT1824591.064371866944504No Hit
TATTGCACTTGTCCCGGCCTGT1694210.9883148875616153No Hit
TTAATGCTAATCGTGATAGGGGTT1578550.9208447983192095No Hit
TATTGCACTTGTCCCGGCCTGTA1440240.8401618652125421No Hit
TGAGGTAGTAGATTGTATAGTT1430350.8343925483994054No Hit
AGCAGCATTGTACAGGGCTATGA1349940.7874854943099894No Hit
CATAAAGTAGAAAGCACTACT1288040.7513762212328243No Hit
CCCATAAAGTAGAAAGCACTAC1103210.6435559152093601No Hit
TGTAAACATCCCCGACTGGAAGCT1102800.6433167423182188No Hit
TTCAAGTAATTCAGGATAGGTT1086100.6335748221180789No Hit
GCCTGTCTGAGCGTCGCT1056100.616074366668726No Hit
CAACGGAATCCCAAAAGCAGCTGT987330.5759574892936589No Hit
TCGTACGACTCTTAGCGG881360.514140047161394No Hit
ACTGGACTTGGAGTCAGAAGGC848910.49521038785034377No Hit
CAACGGAATCCCAAAAGCAGCT844940.49289449424587944No Hit
TAGCTTATCAGACTGATGTTGACA667710.3895076369362513No Hit
TGAGGTAGTAGTTTGTACAGTT658520.38414666408359943No Hit
TGTAAACATCCTACACTCTCAGCT634960.37040297307070746No Hit
CCCATAAAGTAGAAAGCACT633160.3693529457437463No Hit
CACCCGGCTGTGTGCACATGTGC612760.3574526360381862No Hit
CATTGCACTTGTCTCGGTCTGA588710.3434231042529548No Hit
TGTCAGTTTGTCAAATACCCCAA562630.3282093749823173No Hit
CAAAGAATTCTCCTTTTGGGCT561990.32783603193273103No Hit
CCCCCACTGCTAAATTTGACTGGCTT529740.30902304232467653No Hit
AACATTCAACGCTGTCGGTGAGTT528950.30856219699784354No Hit
TCGTTTCCCGGCCAATGCACCA494510.2884716741419862No Hit
CAACACCAGTCGATGGGCTGT479110.2794881070113183No Hit
TTCTCACTACTGCACTTGACTAG456180.2661119255628628No Hit
TGAGAACTGAATTCCATGGGTT424400.24757310975684807No Hit
CTTCTCACTACTGCACTTGACTAG410680.23956956813134395No Hit
TGATGTAGTAGGTTGTATAGTT398600.23252271807040448No Hit
CGCCTGTCTGAGCGTCGCT393280.2294193039707192No Hit
CACTAGATTGTGAGCTCCTGGAA386170.22527169602922248No Hit
TCCTGTCTGAGCGTCGCT385630.22495668783113412No Hit
TGTCAGTTTGTCAAATACCCCA384050.2240349971774682No Hit
TATTGCACTTGTCCCGGCCTGTAG382720.2232591436525469No Hit
CACTAGATTGTGAGCTCCTGGA375740.21918737101799743No Hit
TATTGCACTTGTCCCGGCCTGTT367270.21424640909613007No Hit
TGAGGTAGTAGTTTGTGCTGTT363720.2121755218679566No Hit
ATCCCGGACGAGCCCCCA352440.20559535061899983No Hit
CTCCCACTGCTTCACTTGACTAG347170.20252110394506348No Hit
TAGCAGCACGTAAATATTGGC342180.19961019485532108No Hit
CAAAGAATTCTCCTTTTGGGCTT340970.1989043431521972No Hit
AACGGAATCCCAAAAGCAGCTG326970.1907374639424991No Hit
TTCACAGTGGCTAAGTTCCG311630.1817888977227299No Hit
ACTGGACTTGGAGTCAGAAGGCA311260.18157305877218788No Hit
TGTAAACATCCCCGACTGGAAGC306700.1789129895438862No Hit
CCCCCCACTGCTAAATTTGACTGGCT300580.1753428966322182No Hit
GGAATACCGGGTGCTGTAGGCTTT299730.17484705039448653No Hit
TTCAAGTAATCCAGGATAGGCTT298900.1743628711270544No Hit
TTAATGCTAATCGTGATAGGGGTTT295260.1722394825325329No Hit
AGCAGCATTGTACAGGGCTATGT289070.1686285552248164No Hit
TATTGCACTTGTCCCGGCCTGTTT284330.1658634832638186No Hit
TGATGTAGTAGATTGTATAGTT282520.16480762245170763No Hit
TATTGCACTTGTCCCGGCCTGTAAT279920.1632909163127637No Hit
TCGTTTCCCGGGCGGCGCACCA270960.1580641136185569No Hit
ACTGGACTTGGAGTCAGAAGGCT266330.15536320999420677No Hit
TGAGAACTGAATTCCATAGGCTGT265250.15473319359803006No Hit
TTATAAAGCAATGAGACTGATT260990.1522481289242219No Hit
CCCATAAAGTAGAAAGCACTA260370.1518864528449353No Hit
TATTGCACTTGTCCCGGCCTGTAAA259990.1516647804092435No Hit
CTCCCACTGCTTCACTTGACTAGCCT248050.14469959914040095No Hit
AACCGGGCGGAAACACCA243260.14190535975365426No Hit
TTCAAGTAATCCAGGATAGGC241440.1408436654563935No Hit
CAACGGAATCCCAAAAGCAGCTGA237640.13862694109947543No Hit
AGCTCGGTCTGAGGCCCCTCAGT237320.13844026957468236No Hit
CTACGCCTGTCTGAGCGTCGCT235670.13747774452496794No Hit
CACCCGTAGAACCGACCTTGCG227850.13291595913783658No Hit
AGCTACATCTGGCTACTGGGTCTC224260.130821737969064No Hit
ACCCCCCACTGCTAAATTTGACTG210060.1225381890563702No Hit
CCCCCCACTGCTAAATTTGACTGG202460.11810474034253408No Hit
TAGCAGCACATCATGGTTTACA196590.11468048455961066No Hit
TAGCAGCACGTAAATATTGGCGT196570.11466881758931109No Hit
TGAGGTAGTAGGTTGTGTGGTT195300.11392796497528847No Hit
AACATTCAACGCTGTCGGTGAGA195150.11384046269804173No Hit
AACATTCAACGCTGTCGGTGA194020.11318127887611608No Hit
ATGCACCTGGGCAAGGATTCTGA193190.11269709960868399No Hit
ATCACATTGCCAGGGATTTCC191200.1115362360638769No Hit
TTAATGCTAATCGTGATAGGGGT190520.11113955907369157No Hit
AGCAGCATTGTACAGGGCTATG188930.11021203493487586No Hit
ACCACAGGGTAGAACCACGGAC187730.10951201671690174No Hit
TGTAAACATCCTACACTCAGCT187100.1091445071524653No Hit
ACTGGACTTGGAGTCAGAAGG186540.10881783198407738No Hit
GGAATACCGGGTGCTGTAGGCTT185650.10829865180574658No Hit
ATCACATTGCCAGGGATTTCCA185130.10799531057795779No Hit
TTCACAGTGGCTAAGTTCTGC183610.10710862083519057No Hit
CAAAGTGCTGTTCGTGCAGGTAG180620.10536440877540504No Hit
ACTTGACTTGGAGTCAGAAGGC178630.10420354523059795No Hit
AGCTACATTGTCTGCTGGGTTT176580.10300768077489215No Hit
TGAGGTAGTAGGTTGTATAGT174650.10188181814098378No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)