FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9549678
Sequences flagged as poor quality0
Sequence length18-26
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7627827.987515390571284No Hit
TTCAAGTAATCCAGGATAGGCT4838255.066401191746989No Hit
TAGCAGCACGTAAATATTGGCG3714733.889900790372199No Hit
AAGCTGCCAGTTGAAGAACTGT3571003.7393930978615195No Hit
AACATTCAACGCTGTCGGTGAGT2969543.109570814848417No Hit
CCCCCCACTGCTAAATTTGACTG2941683.0803970563195953No Hit
TAGCTTATCAGACTGATGTTGA2774332.905155545558709No Hit
TAGCTTATCAGACTGATGTTGAC2678872.8051940599463143No Hit
TAGCTTATCAGACTGATGTTGACT2543652.663597662664647No Hit
AACATTCAACGCTGTCGGTGAG2025612.121129110321835No Hit
CATAAAGTAGAAAGCACTACT1767721.8510781201209088No Hit
TGAGGTAGTAGGTTGTATAGTT1662771.741179126667936No Hit
CCCCCACTGCTAAATTTGACTG1201331.2579795884217249No Hit
TATTGCACTTGTCCCGGCCTGTAA1192461.248691317131321No Hit
TGAGGTAGTAGATTGTATAGTT1172481.2277691457240758No Hit
TATTGCACTTGTCCCGGCCTGTAT1096461.148164367426839No Hit
TGTAAACATCCTTGACTGGAAGCT1053541.1032204436631265No Hit
TATTGCACTTGTCCCGGCCTGT689290.7217939704354429No Hit
TTCAAGTAATTCAGGATAGGTT689130.7216264255192688No Hit
CCCATAAAGTAGAAAGCACTAC603620.6320841393814535No Hit
TGAGGTAGTAGTTTGTACAGTT552860.5789305147252085No Hit
AGCAGCATTGTACAGGGCTATGA548620.5744905744465939No Hit
GGAATACCGGGTGCTGTAGGCTTT515150.5394422722944167No Hit
TGTAAACATCCCCGACTGGAAGCT508780.5327718903192338No Hit
TATTGCACTTGTCCCGGCCTGTA469360.49149301159683084No Hit
CCCATAAAGTAGAAAGCACT462490.4842990517586038No Hit
CAACGGAATCCCAAAAGCAGCTGT461080.4828225621848192No Hit
TGTAAACATCCTACACTCTCAGCT437210.4578269550030901No Hit
ACTGGACTTGGAGTCAGAAGGC421140.4409991624848503No Hit
TTCTCACTACTGCACTTGACTAG405690.4248206065167852No Hit
CAACACCAGTCGATGGGCTGT370660.3881387414319101No Hit
CATTGCACTTGTCTCGGTCTGA348910.3653631043894883No Hit
CCCCCACTGCTAAATTTGACTGGCTT341620.3577293391463042No Hit
CAACGGAATCCCAAAAGCAGCT339840.35586540195386696No Hit
GGAATACCGGGTGCTGTAGGCTT323740.3390061947638444No Hit
TAGCTTATCAGACTGATGTTGACA304140.3184819425325126No Hit
CTCCCACTGCTTCACTTGACTAG288100.3016855646860554No Hit
CTTCTCACTACTGCACTTGACTAG283650.29702572170496216No Hit
CAAAGAATTCTCCTTTTGGGCT281320.29458584886317635No Hit
TTCACAGTGGCTAAGTTCCG279050.29220880536495575No Hit
CACCCGGCTGTGTGCACATGTGC270810.2835802421819877No Hit
TGTAAACATCCTACACTCAGCT245760.25734899124347443No Hit
TTCAAGTAATCCAGGATAGGC243060.25452167078303584No Hit
TTCACAGTGGCTAAGTTCCGC237830.24904504633559374No Hit
TGAGGTAGTAGTTTGTGCTGTT237800.24901363166381107No Hit
AGAATTACTCTGAGACCT218920.2292433315552629No Hit
CGCCTGTCTGAGCGTCGCT208760.2186042293782052No Hit
TAGCAGCACGTAAATATTGGC208600.21843668446203107No Hit
TTATAAAGCAATGAGACTGATT208310.21813300930146545No Hit
AACGGAATCCCAAAAGCAGCTG204340.2139758010688947No Hit
TGAATACCGGGTGCTGTAGGCTTT203170.2127506288693713No Hit
AACATTCAACGCTGTCGGTGAGTT194510.20368226028144612No Hit
TTCACAGTGGCTAAGTTCTGC191090.20010098769822396No Hit
TTCAAGTAATCCAGGATAGGCTT190350.1993260924609186No Hit
TCGTTTCCCGGCCAATGCACCA183340.1919855308210392No Hit
TGTCAGTTTGTCAAATACCCCA178490.18690682554951069No Hit
CAAAGAATTCTCCTTTTGGGCTT168540.17648762607493151No Hit
TAGCAGCACATCATGGTTTACA167320.17521009608910373No Hit
TTCACAGTGGCTAAGTTCTG165590.17339851668297088No Hit
CCCCCCACTGCTAAATTTGACTGGCT164740.17250843431579577No Hit
CTACGCCTGTCTGAGCGTCGCT164050.1717858968647948No Hit
TGTAAACATCCCCGACTGGAAGC161560.1691784791068348No Hit
GTCAGGATGGCCGAGCGGTCTAAGG157100.16450816456848072No Hit
TCAGTGCACTACAGAACTTTGT155580.16291648786482643No Hit
TGTCAGTTTGTCAAATACC153540.1607802901836062No Hit
ATCACATTGCCAGGGATTTCC153300.160528972809345No Hit
TGAGGTAGTAGGTTGTATAGT147440.15439264025446722No Hit
CCCATAAAGTAGAAAGCACTA142730.14946053678459106No Hit
TCGTACGACTCTTAGCGG141970.1486646984327639No Hit
TGATGTAGTAGGTTGTATAGTT140450.1470730217291096No Hit
TGTCAGTTTGTCAAATACCCCAA138860.14540804412462913No Hit
TAGCTTATCAGACTGATGTTG137440.14392108299358367No Hit
AGCTCGGTCTGAGGCCCCTCAGT134710.14106234786136243No Hit
TGAGGTAGTAAGTTGTATTGTT134140.14046546909749208No Hit
CACTAGATTGTGAGCTCCTGGA133460.13975340320375199No Hit
ACTGGACTTGGAGTCAGAAGGCT132320.13855964567601128No Hit
TATTGCACTTGTCCCGGCCTGTT132300.1385387025614895No Hit
CACTAGATTGTGAGCTCCTGGAA129390.1354914793985724No Hit
TGTCAGTTTGTCAAATACCCC129010.1350935602226588No Hit
TGAATACCGGGTGCTGTAGGCTT127770.13379508712230925No Hit
AGAGGTAGTAGGTTGCATAGTT126480.13244425623565528No Hit
GCCTGTCTGAGCGTCGCT125730.131658889441089No Hit
TAGCAGCACATAATGGTTTGT125580.13150181608217576No Hit
CTCCCACTGCTTCACTTGACTAGCCT123680.12951222020260786No Hit
TATTGCACTTGTCCCGGCCTGTAG122550.12832893423212804No Hit
TGAGGTAGTAGGTTGTGTGGTT119080.12469530386260144No Hit
ACTGGACTTGGAGTCAGAAGG117810.12336541609046923No Hit
AACATTCAACGCTGTCGGTGA115680.12113497439390103No Hit
CAAAGTGCTGTTCGTGCAGGT110490.11570023617550247No Hit
ACTGGACTTGGAGTCAGAAGGCA108560.11367922562415193No Hit
CCCCCCACTGCTAAATTTGACTGG108250.11335460734906455No Hit
AGCAGCATTGTACAGGGCTATGT107950.11304046063123803No Hit
TAGCAGCACGTAAATATTGGCGT107440.11250641121093297No Hit
TCAGTGCACTACAGAACTTTGTC106410.11142784081306197No Hit
TCAGTGCATCACAGAACTTTGTTT106080.11108227942345282No Hit
TGTCAGTTTGTCAAATACCC105350.1103178557434083No Hit
TGAGGTAGTAGATTGTATAGT103320.10819212961944895No Hit
TGATGTAGTAGATTGTATAGTT100260.10498783309761858No Hit
CATAAAGTAGAAAGCACTAC98920.10358464442466017No Hit
ACCGGGTGCTGTAGGCTT98770.10342757106574693No Hit
TACGCCTGTCTGAGCGTCGCT97000.10157410543057055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)