FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10885518
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6694486.149895668722426No Hit
CCCCCCACTGCTAAATTTGACTG5844915.369436713990092No Hit
AAGCTGCCAGTTGAAGAACTGT4916834.516854411521804No Hit
TTCAAGTAATCCAGGATAGGCT4802854.412146486735863No Hit
TAGCAGCACGTAAATATTGGCG4204043.86204864113954No Hit
AACATTCAACGCTGTCGGTGAGT3275173.0087406038003888No Hit
TAGCTTATCAGACTGATGTTGAC3264362.998809978542133No Hit
TAGCTTATCAGACTGATGTTGACT2196352.0176807387576776No Hit
TGTAAACATCCTTGACTGGAAGCT1973171.8126560444803823No Hit
TAGCTTATCAGACTGATGTTGA1810621.663329204912435No Hit
AACATTCAACGCTGTCGGTGAG1809131.6619604138268844No Hit
CCCCCACTGCTAAATTTGACTG1727021.5865299198439615No Hit
TATTGCACTTGTCCCGGCCTGTAA1527401.4031486604495993No Hit
GCCTGTCTGAGCGTCGCT1352481.2424580989163767No Hit
TATTGCACTTGTCCCGGCCTGTAT1331281.2229826821286778No Hit
TGAGGTAGTAGGTTGTATAGTT1294061.1887904645419722No Hit
CATAAAGTAGAAAGCACTACT1168731.0736558425607305No Hit
TTCAAGTAATTCAGGATAGGTT1151271.0576161832629372No Hit
TGAGGTAGTAGATTGTATAGTT1030910.9470472604059816No Hit
AGCAGCATTGTACAGGGCTATGA975000.8956854418871018No Hit
TATTGCACTTGTCCCGGCCTGT972710.8935817294133362No Hit
AACCGGGCGGAAACACCA907270.8334651598573445No Hit
TGTAAACATCCTACACTCTCAGCT816620.7501893800552256No Hit
TGTAAACATCCCCGACTGGAAGCT752310.6911108869600877No Hit
TATTGCACTTGTCCCGGCCTGTA631490.5801193843049086No Hit
ACTGGACTTGGAGTCAGAAGGC601090.5521923715527364No Hit
CCCATAAAGTAGAAAGCACTAC557210.5118819334091405No Hit
GGAATACCGGGTGCTGTAGGCTTT508570.46719871300566496No Hit
TTCTCACTACTGCACTTGACTAG487510.44785190746090353No Hit
TGAGGTAGTAGTTTGTACAGTT438790.4030951949186065No Hit
CAACGGAATCCCAAAAGCAGCTGT428050.3932288752818194No Hit
TAGCTTATCAGACTGATGTTGACA398130.36574281536257625No Hit
CGCCTGTCTGAGCGTCGCT374380.3439248366499417No Hit
CTCCCACTGCTTCACTTGACTAG368940.3389273712100793No Hit
CTTCTCACTACTGCACTTGACTAG366860.3370165756007201No Hit
CATTGCACTTGTCTCGGTCTGA364860.3351792721301825No Hit
TGTAAACATCCTACACTCAGCT362120.3326621663755459No Hit
TGTCAGTTTGTCAAATACCCCAA341490.31371038107695015No Hit
CAAAGAATTCTCCTTTTGGGCT340980.313241868691963No Hit
CCCATAAAGTAGAAAGCACT340520.3128192888937394No Hit
GGAATACCGGGTGCTGTAGGCTT323100.29681637566535646No Hit
TGTCAGTTTGTCAAATACCCCA323090.2968071891480038No Hit
TCGTACGACTCTTAGCGG313210.28773091000354784No Hit
AGCTCGGTCTGAGGCCCCTCAGT298300.2740338126306897No Hit
TTCAAGTAATCCAGGATAGGCTT296040.27195765970898217No Hit
CACCCGGCTGTGTGCACATGTGC283670.2605939377437068No Hit
AACATTCAACGCTGTCGGTGAGTT274790.2524363103345197No Hit
CAAAGAATTCTCCTTTTGGGCTT271470.24938638657342718No Hit
TGAGGTAGTAGTTTGTGCTGTT252590.23204224181155184No Hit
TGTCAGTTTGTCAAATACCCC243090.223315050326498No Hit
CCCCCCACTGCTAAATTTGACTGGCT242970.22320481211826576No Hit
CCCCCACTGCTAAATTTGACTGGCTT242560.22282816490680554No Hit
TGTAAACATCCCCGACTGGAAGC238050.21868504558074314No Hit
TTATAAAGCAATGAGACTGATT227540.2090300158430678No Hit
TTCACAGTGGCTAAGTTCCG227150.208671741666313No Hit
TAGCAGCACGTAAATATTGGC211540.19433158807876666No Hit
TTCAAGTAATCCAGGATAGGC210680.1935415475864355No Hit
AGCAGCATTGTACAGGGCTATGT201800.18538392017724833No Hit
CTACGCCTGTCTGAGCGTCGCT200930.18458469316756446No Hit
TACGCCTGTCTGAGCGTCGCT192190.17655567700131494No Hit
TGTAAACATCCTTGACTGGAAGC183550.16861852600859234No Hit
ATCACATTGCCAGGGATTTCCA182810.1679387237244934No Hit
CAACACCAGTCGATGGGCTGT181880.1670843776106934No Hit
CCCCCCACTGCTAAATTTGACTGG180880.16616572587542458No Hit
TTCACAGTGGCTAAGTTCCGC180830.16611979328866114No Hit
CAACGGAATCCCAAAAGCAGCT180310.16564209438632135No Hit
AACGGAATCCCAAAAGCAGCTG175440.16116826043556218No Hit
TCAGTGCACTACAGAACTTTGT173880.15973516372854282No Hit
TATTGCACTTGTCCCGGCCTGTAG170230.15638208489481162No Hit
TTCACAGTGGCTAAGTTCTGC165480.15201848915228472No Hit
TATTGCACTTGTCCCGGCCTGTT161450.14831632265915135No Hit
TGATGTAGTAGGTTGTATAGTT152010.13964425027821367No Hit
CTCCCACTGCTTCACTTGACTAGCCT149090.13696178721122873No Hit
ATCACATTGCCAGGGATTTCC148040.13599720288919645No Hit
TAGCAGCACGTAAATATTGGCGT146350.13444468145659214No Hit
CAAAGTGCTGTTCGTGCAGGTAG142430.13084356665433836No Hit
CACTAGATTGTGAGCTCCTGGAA138130.12689336419268243No Hit
ACCCCCCACTGCTAAATTTGACTG136730.1256072517633061No Hit
AGCAGCATTGTACAGGGCTATG135300.12429357978187167No Hit
CACTAGATTGTGAGCTCCTGGA134250.12332899545983939No Hit
TGAGGTAGTAGGTTGTGTGGTT131380.12069246497961787No Hit
CAAAGTGCTGTTCGTGCAGGT130760.12012290090375122No Hit
GCGTTCGCGCTTTCCCCTG129760.11920424916848238No Hit
TTCAAGTAATTCAGGATAGGTTA127870.11746799738882431No Hit
TTCAAGTAATCCAGGATAGGCTA127590.11721077490294905No Hit
TCCTGTCTGAGCGTCGCT125430.1152264871547684No Hit
TGTCAGTTTGTCAAATACCC123450.1134075567189361No Hit
AGAGGTAGTAGGTTGCATAGTT123100.11308602861159203No Hit
TGATGTAGTAGATTGTATAGTT121490.11160699931780922No Hit
TCGTTTCCCGGGCGGCGCACCA119130.10943898122257481No Hit
ACGCCTGTCTGAGCGTCGCT117710.10813449575849307No Hit
TGAGGTAGTAGGTTGTATAGT117490.10793239237673394No Hit
ACCACAGGGTAGAACCACGGAC117130.10760167775203716No Hit
TATTGCACTTGTCCCGGCCTGTAAT115920.10649010915236189No Hit
CCCATAAAGTAGAAAGCACTA114950.10559901696915112No Hit
CAGTGCAATAGTATTGTCAAAGC113070.10387195170684572No Hit
TATTGCACTTGTCCCGGCCTGTTT110680.10167637405955325No Hit
GTAAACATCCTTGACTGGAAGCT110110.10115274257045002No Hit
TAGCAGCACATAATGGTTTGT109550.1006382975986995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)