FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6774677
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6636439.795935658629924No Hit
CCCCCCACTGCTAAATTTGACTG4687786.919562364375453No Hit
GCCTGTCTGAGCGTCGCT2950554.355262988921833No Hit
TTCAAGTAATCCAGGATAGGCT2466413.6406311326724508No Hit
AACATTCAACGCTGTCGGTGAGT2309703.4093138314933684No Hit
AAGCTGCCAGTTGAAGAACTGT2092503.088708140624269No Hit
TAGCAGCACGTAAATATTGGCG1980752.923755627021037No Hit
AACATTCAACGCTGTCGGTGAG1770642.613615379744304No Hit
CCCCCACTGCTAAATTTGACTG1472292.173225380339166No Hit
TATTGCACTTGTCCCGGCCTGTAA1273151.879277786970508No Hit
TATTGCACTTGTCCCGGCCTGTAT1024661.5124853922925034No Hit
TGAGGTAGTAGGTTGTATAGTT949861.4020742243504745No Hit
TAGCTTATCAGACTGATGTTGAC932211.3760213217545278No Hit
TAGCTTATCAGACTGATGTTGACT890241.314070028726093No Hit
TAGCTTATCAGACTGATGTTGA708381.0456291864542029No Hit
TGTAAACATCCTTGACTGGAAGCT707141.0437988408893883No Hit
TGAGGTAGTAGATTGTATAGTT649410.9585844461662157No Hit
TATTGCACTTGTCCCGGCCTGT564600.8333976660437096No Hit
TTCAAGTAATTCAGGATAGGTT504230.7442864065696416No Hit
TATTGCACTTGTCCCGGCCTGTA476990.7040778475490418No Hit
AACCGGGCGGAAACACCA426070.628915592581019No Hit
AGCAGCATTGTACAGGGCTATGA417070.6156308263847856No Hit
CATAAAGTAGAAAGCACTACT404570.5971797622233502No Hit
TGTAAACATCCCCGACTGGAAGCT379670.5604252424137711No Hit
CAACGGAATCCCAAAAGCAGCTGT349740.5162460143856306No Hit
ACTGGACTTGGAGTCAGAAGGC323400.47736593198465405No Hit
CCCATAAAGTAGAAAGCACTAC307240.45351239623675044No Hit
CACCCGGCTGTGTGCACATGTGC304620.44964505318851367No Hit
CAACGGAATCCCAAAAGCAGCT292170.43126779328372405No Hit
TCCTGTCTGAGCGTCGCT269360.3975982914019369No Hit
TGAGGTAGTAGTTTGTACAGTT256330.3783649021200568No Hit
TTAATGCTAATCGTGATAGGGGTT248110.3662314823274969No Hit
TGAGAACTGAATTCCATGGGTT228480.337255931168379No Hit
TGAGAACTGAATTCCATAGGCTGT220050.32481253349790695No Hit
CCCCCACTGCTAAATTTGACTGGCTT219870.3245468381739823No Hit
TGTAAACATCCTACACTCTCAGCT206930.30544629655406447No Hit
GGAATACCGGGTGCTGTAGGCTTT202820.2993795866577846No Hit
CCCATAAAGTAGAAAGCACT198160.2925010299384015No Hit
CATTGCACTTGTCTCGGTCTGA188640.2784486994730524No Hit
TTCTCACTACTGCACTTGACTAG186690.2755703334638685No Hit
CTCCCACTGCTTCACTTGACTAG184100.27174727296961904No Hit
CAAAGAATTCTCCTTTTGGGCT183470.2708173393358827No Hit
TAGCAGCACGTAAATATTGGC178740.26383545665719565No Hit
AACGGAATCCCAAAAGCAGCTG168410.2485874972341855No Hit
TAGCTTATCAGACTGATGTTGACA163540.2413989626368903No Hit
CGCCTGTCTGAGCGTCGCT162640.24007048601726697No Hit
TGAGGTAGTAGTTTGTGCTGTT152150.22458635297299043No Hit
AGCTCGGTCTGAGGCCCCTCAGT150630.2223427035709599No Hit
TATTGCACTTGTCCCGGCCTGTAG146450.21617266771537594No Hit
TCGTTTCCCGGCCAATGCACCA145420.21465230002847366No Hit
AACATTCAACGCTGTCGGTGAGTT138170.20395068281484122No Hit
CTTCTCACTACTGCACTTGACTAG137050.2022974674659766No Hit
GGGAATACCGGGTGCTGTAGGCTTT133870.19760351674330745No Hit
CTACGCCTGTCTGAGCGTCGCT133720.19738210397337025No Hit
GGAATACCGGGTGCTGTAGGCTT131290.19379521710038722No Hit
CAAAGAATTCTCCTTTTGGGCTT129940.1918025021709522No Hit
CCCCCCACTGCTAAATTTGACTGGCT124730.18411209862846598No Hit
CACTAGATTGTGAGCTCCTGGAA121900.17993477770231703No Hit
CACTAGATTGTGAGCTCCTGGA121160.17884247470396006No Hit
TTCAAGTAATCCAGGATAGGCTT119360.17618552146471336No Hit
CAACACCAGTCGATGGGCTGT117570.17354332907679584No Hit
ACCCCCCACTGCTAAATTTGACTG113290.16722568470792037No Hit
CCCCCCACTGCTAAATTTGACTGG110000.16236936462063062No Hit
TCGTACGACTCTTAGCGG109910.1622365169586683No Hit
TGTAAACATCCCCGACTGGAAGC108920.1607751926770826No Hit
ACTGGACTTGGAGTCAGAAGGCA106190.15674548026422516No Hit
TATTGCACTTGTCCCGGCCTGTT101240.14943885885629676No Hit
ACTGGACTTGGAGTCAGAAGGCT100180.14787420861540707No Hit
TATTGCACTTGTCCCGGCCTGTAAT99730.14720997030559538No Hit
CACCCGTAGAACCGACCTTGCG99360.1466638188064169No Hit
TATTGCACTTGTCCCGGCCTGTAAA98500.14539438559211015No Hit
CTCCCACTGCTTCACTTGACTAGCCT98200.1449515600522357No Hit
TTCAAGTAATCCAGGATAGGC97580.1440363872698285No Hit
TTCACAGTGGCTAAGTTCCGC94270.13915054547988046No Hit
TTCACAGTGGCTAAGTTCTGC93980.13872248079133515No Hit
GGGAATACCGGGTGCTGTAGGCTT93070.13737924332038265No Hit
CAACGGAATCCCAAAAGCAGCTGA91690.1353422458369602No Hit
TGTCAGTTTGTCAAATACCCCA91340.13482561604044002No Hit
TGAGGTAGTAGGTTGTGTGGTT90900.13417613858195748No Hit
TGTCAGTTTGTCAAATACCCCAA89780.1325229232330929No Hit
TACGCCTGTCTGAGCGTCGCT84770.12512773671718963No Hit
AGCAGCATTGTACAGGGCTATGT84340.12449302011003625No Hit
ACCGGGTGCTGTAGGCTTA80930.11945956980679669No Hit
ATCACATTGCCAGGGATTTCC79510.11736352891805765No Hit
TGATGTAGTAGGTTGTATAGTT76990.1136437943831123No Hit
TGAGGTAGTAAGTTGTATTGTT76650.11314192543792125No Hit
TCGTTTCCCGGGCGGCGCACCA76210.11249244797943872No Hit
TATTGCACTTGTCCCGGCCTGTTT75220.11103112369785305No Hit
TGAGGTAGTAGGTTGTATAGT74880.11052925475266201No Hit
TTATAAAGCAATGAGACTGATT73760.10887603940379742No Hit
TAGCAGCACGTAAATATTGGCGT73070.10785754066208618No Hit
TTCACAGTGGCTAAGTTCCG72600.10716378064961621No Hit
TCAGTGCACTACAGAACTTTGT71740.10589434743530947No Hit
ACCGGGTGCTGTAGGCTT71560.10562865211138478No Hit
ACCGGGTGCTGTAGGCTTT69790.10301598142612556No Hit
AATGCACCCGGGCAAGGATTCT69760.10297169887213813No Hit
CCCATAAAGTAGAAAGCACTA68150.10059520180814524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)