FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8154673
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG89457110.970041349297514No Hit
CCCCCCACTGCTAAATTTGACTG5715647.009036413845165No Hit
AACATTCAACGCTGTCGGTGAGT2893123.547806270098139No Hit
TTCAAGTAATCCAGGATAGGCT2697733.3082013221131No Hit
AAGCTGCCAGTTGAAGAACTGT2606603.196449446838641No Hit
TAGCAGCACGTAAATATTGGCG2344952.875590474320675No Hit
GCCTGTCTGAGCGTCGCT2023092.48089653625596No Hit
AACATTCAACGCTGTCGGTGAG1991332.441949542305375No Hit
CCCCCACTGCTAAATTTGACTG1845532.2631563521921727No Hit
TATTGCACTTGTCCCGGCCTGTAA1497941.8369099533482214No Hit
TGAGGTAGTAGGTTGTATAGTT1164311.4277825732558498No Hit
TAGCTTATCAGACTGATGTTGAC1130331.3861132138590966No Hit
TATTGCACTTGTCCCGGCCTGTAT1121121.3748190761297234No Hit
TAGCTTATCAGACTGATGTTGACT995191.220392283050467No Hit
TCCTGTCTGAGCGTCGCT859831.0544015682787036No Hit
TATTGCACTTGTCCCGGCCTGT771770.946414405580702No Hit
TAGCTTATCAGACTGATGTTGA762540.9350957420364986No Hit
TGTAAACATCCTTGACTGGAAGCT668820.8201677737415098No Hit
TGAGGTAGTAGATTGTATAGTT588450.7216107868457754No Hit
TTAATGCTAATCGTGATAGGGGTT586850.719648721659348No Hit
AGCAGCATTGTACAGGGCTATGA551360.6761276632429039No Hit
TATTGCACTTGTCCCGGCCTGTA541110.6635581831423529No Hit
CAACGGAATCCCAAAAGCAGCTGT504320.6184429467619363No Hit
ACTGGACTTGGAGTCAGAAGGC497150.6096504421452583No Hit
AACCGGGCGGAAACACCA467340.5730947151406316No Hit
CATAAAGTAGAAAGCACTACT420150.5152260550484367No Hit
CACCCGGCTGTGTGCACATGTGC416890.5112283472310907No Hit
TTCAAGTAATTCAGGATAGGTT396140.4857828143446095No Hit
CCCATAAAGTAGAAAGCACTAC380080.4660885850358439No Hit
TGTAAACATCCCCGACTGGAAGCT377850.4633539566822606No Hit
CAACGGAATCCCAAAAGCAGCT357100.43790842379577943No Hit
TCGTACGACTCTTAGCGG351690.43127419088417157No Hit
CGCCTGTCTGAGCGTCGCT340420.41745389422727314No Hit
CCCATAAAGTAGAAAGCACT248490.3047209863596002No Hit
AACGGAATCCCAAAAGCAGCTG240060.2943833554086105No Hit
CATTGCACTTGTCTCGGTCTGA234580.2876632821450964No Hit
AGCTCGGTCTGAGGCCCCTCAGT230400.2825373868455547No Hit
CCCCCACTGCTAAATTTGACTGGCTT226950.2783066837873205No Hit
TGTAAACATCCTACACTCTCAGCT225820.27692097524940607No Hit
TGAGAACTGAATTCCATAGGCTGT204890.2512547100294518No Hit
CTCCCACTGCTTCACTTGACTAG199490.24463274002525914No Hit
TGAGGTAGTAGTTTGTACAGTT199070.24411769791382196No Hit
CACCCGTAGAACCGACCTTGCG189930.23290940053635506No Hit
AACATTCAACGCTGTCGGTGAGTT186580.22880132655227256No Hit
TAGCTTATCAGACTGATGTTGACA179320.21989845576885791No Hit
CAAAGAATTCTCCTTTTGGGCT166530.2042141971848534No Hit
TTCTCACTACTGCACTTGACTAG164920.20223986909101077No Hit
TATTGCACTTGTCCCGGCCTGTAG163370.20033911844165916No Hit
TGAGGTAGTAGGTTGTGTGGTT161310.1978129595141338No Hit
ACCCCCCACTGCTAAATTTGACTG158790.19472270684551055No Hit
TAGCAGCACGTAAATATTGGC155480.19066368449108873No Hit
TGAGGTAGTAGTTTGTGCTGTT152420.1869112348220462No Hit
CAACACCAGTCGATGGGCTGT149270.18304841898626714No Hit
TCGTTTCCCGGCCAATGCACCA149180.1829380528195306No Hit
TGAGAACTGAATTCCATGGGTT146330.17944312420620667No Hit
CTACGCCTGTCTGAGCGTCGCT145320.17820457055727434No Hit
CACTAGATTGTGAGCTCCTGGA141050.17296830909099603No Hit
TACGCCTGTCTGAGCGTCGCT140640.172465529886974No Hit
CACTAGATTGTGAGCTCCTGGAA140550.17235516372023746No Hit
CAACGGAATCCCAAAAGCAGCTGA138410.16973090153339074No Hit
TTCAAGTAATCCAGGATAGGCTT137510.16862723986602526No Hit
CCCCCCACTGCTAAATTTGACTGGCT132710.1627410443067429No Hit
ACCGGGTGCTGTAGGCTT129920.15931969313791No Hit
TATTGCACTTGTCCCGGCCTGTT128560.15765193772944666No Hit
TATTGCACTTGTCCCGGCCTGTAAA126290.1548682577462027No Hit
ACCGGGTGCTGTAGGCTTA125410.15378912189366759No Hit
CCCCCCACTGCTAAATTTGACTGG124470.15263640859664146No Hit
CTTCTCACTACTGCACTTGACTAG121980.1495829446502637No Hit
ACCGGGTGCTGTAGGCTTT117740.14438347190623094No Hit
AATGCACCCGGGCAAGGATTCT115930.1421638856640849No Hit
CAAAGAATTCTCCTTTTGGGCTT115890.14211483403442418No Hit
TATTGCACTTGTCCCGGCCTGTAAT113890.13966225255138986No Hit
TTCAAGTAATCCAGGATAGGC113870.13963772673655953No Hit
TTCACAGTGGCTAAGTTCTGC111370.1365719998827666No Hit
GGAATACCGGGTGCTGTAGGCTTT105690.12960666847094912No Hit
AGCAGCATTGTACAGGGCTATGT105560.1294472506745519No Hit
TGTAAACATCCCCGACTGGAAGC105240.12905483763726638No Hit
CTCCCACTGCTTCACTTGACTAGCCT101550.12452982480106806No Hit
ACTGGACTTGGAGTCAGAAGGCT93890.11513643772104658No Hit
TCGTTTCCCGGGCGGCGCACCA93500.11465818433185487No Hit
ACCACAGGGTAGAACCACGGAC93480.11463365851702453No Hit
TGAGGTAGTAAGTTGTATTGTT88220.10818336921664426No Hit
TGTCAGTTTGTCAAATACCCCAA87680.107521172216225No Hit
CGCGACCTCAGATCAGACGTGGCG84890.10409982104739209No Hit
ACTGGACTTGGAGTCAGAAGGCA84660.10381777417684314No Hit
TGAGGTAGTAGGTTGTATAGT84410.10351120149146384No Hit
ACGCCTGTCTGAGCGTCGCT84320.10340083532472731No Hit
ATCACATTGCCAGGGATTTCC83060.10185570899041568No Hit
TTATAAAGCAATGAGACTGATT82100.1006784698785592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)