FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9824598
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG101114910.292013983676481No Hit
CCCCCCACTGCTAAATTTGACTG6443786.55882306838407No Hit
AACATTCAACGCTGTCGGTGAGT3839473.908017406920874No Hit
TTCAAGTAATCCAGGATAGGCT3465803.527676145120645No Hit
TAGCAGCACGTAAATATTGGCG3165163.2216687135697564No Hit
AACATTCAACGCTGTCGGTGAG2997653.0511680986845464No Hit
AAGCTGCCAGTTGAAGAACTGT2585452.6316089472566717No Hit
CCCCCACTGCTAAATTTGACTG1990512.0260472744024742No Hit
TATTGCACTTGTCCCGGCCTGTAA1464841.4909923031965278No Hit
TGAGGTAGTAGGTTGTATAGTT1373451.3979706854163396No Hit
GCCTGTCTGAGCGTCGCT1187561.2087619259332545No Hit
TAGCTTATCAGACTGATGTTGAC1182841.2039576581148663No Hit
TTAATGCTAATCGTGATAGGGGTT1179321.2003748143181023No Hit
TATTGCACTTGTCCCGGCCTGTAT1106961.1267229458141697No Hit
TATTGCACTTGTCCCGGCCTGT1033311.0517580464869911No Hit
TAGCTTATCAGACTGATGTTGA983761.0013234129274298No Hit
TAGCTTATCAGACTGATGTTGACT886280.9021030682374994No Hit
TTCAAGTAATTCAGGATAGGTT776450.7903122346583545No Hit
CAACGGAATCCCAAAAGCAGCT759980.7735481899615638No Hit
TATTGCACTTGTCCCGGCCTGTA716160.7289458561052574No Hit
TGAGGTAGTAGATTGTATAGTT704610.7171896498971255No Hit
TGTAAACATCCTTGACTGGAAGCT660700.6724957092391973No Hit
AGCAGCATTGTACAGGGCTATGA646800.658347547655385No Hit
CAACGGAATCCCAAAAGCAGCTGT562230.5722676897314272No Hit
CATAAAGTAGAAAGCACTACT560880.5708935877071001No Hit
TCGTACGACTCTTAGCGG552870.5627405823627593No Hit
TGAGAACTGAATTCCATGGGTT493210.5020154514210149No Hit
ACTGGACTTGGAGTCAGAAGGC472130.48055910277448505No Hit
TGTAAACATCCCCGACTGGAAGCT429330.43699497933655906No Hit
CACCCGGCTGTGTGCACATGTGC410550.4178796933981421No Hit
CCCATAAAGTAGAAAGCACTAC403950.41116186127920956No Hit
TGAGAACTGAATTCCATAGGCTGT353620.3599333021055925No Hit
CCCCCACTGCTAAATTTGACTGGCTT339300.3453576421142117No Hit
CTCCCACTGCTTCACTTGACTAG326070.3318914422758061No Hit
AACCGGGCGGAAACACCA311860.3174277461530742No Hit
CATTGCACTTGTCTCGGTCTGA305600.3110559841736018No Hit
TCGTTTCCCGGCCAATGCACCA295930.30121334226601437No Hit
TGAGGTAGTAGTTTGTACAGTT292680.2979053188741158No Hit
CCCATAAAGTAGAAAGCACT282220.2872585728189591No Hit
AACGGAATCCCAAAAGCAGCTG272390.27725307437515506No Hit
ACCGGGTGCTGTAGGCTT270700.27553290221136784No Hit
AGCTCGGTCTGAGGCCCCTCAGT267320.27209255788379333No Hit
TGAGGTAGTAGGTTGTGTGGTT249650.2541070891653786No Hit
TGTAAACATCCTACACTCTCAGCT246940.25134870658321085No Hit
AACATTCAACGCTGTCGGTGAGTT244400.24876335907077318No Hit
CGCCTGTCTGAGCGTCGCT239430.24370462791454672No Hit
CAAAGAATTCTCCTTTTGGGCT238910.24317534417184297No Hit
TAGCAGCACGTAAATATTGGC236670.24089535266481132No Hit
TTCTCACTACTGCACTTGACTAG232230.23637608378480218No Hit
CCCCCCACTGCTAAATTTGACTGGCT223620.22761236642964933No Hit
ACCGGGTGCTGTAGGCTTT220500.2244366639734267No Hit
TAGCTTATCAGACTGATGTTGACA216800.22067060657341908No Hit
ACCGGGTGCTGTAGGCTTA213330.21713865544422273No Hit
TGAGGTAGTAGTTTGTGCTGTT211140.2149095566047588No Hit
CAACACCAGTCGATGGGCTGT209190.21292474256961966No Hit
CAACGGAATCCCAAAAGCAGCTGA204120.20776422607825784No Hit
CACCCGTAGAACCGACCTTGCG199570.20313299332959983No Hit
CACTAGATTGTGAGCTCCTGGA192730.19617087640634254No Hit
TTAATGCTAATCGTGATAGGGGT179330.18253164149820686No Hit
GGAATACCGGGTGCTGTAGGCTTT174400.17751362447603453No Hit
TTCACAGTGGCTAAGTTCCG173840.17694362659927662No Hit
TTCAAGTAATCCAGGATAGGC173340.17643469992359992No Hit
TATTGCACTTGTCCCGGCCTGTT173050.17613952245170744No Hit
CTACGCCTGTCTGAGCGTCGCT171890.17495881256413748No Hit
ACCCCCCACTGCTAAATTTGACTG171330.17438881468737957No Hit
TATTGCACTTGTCCCGGCCTGTAG167680.17067364995493964No Hit
CTCCCACTGCTTCACTTGACTAGCCT167500.17049043635169603No Hit
CCCCCCACTGCTAAATTTGACTGG166020.16898401339169297No Hit
ATCCCGGACGAGCCCCCA162720.16562509733222672No Hit
CTTCTCACTACTGCACTTGACTAG158600.16143154152465067No Hit
CACTAGATTGTGAGCTCCTGGAA157390.16019993896951307No Hit
GACCCGCGGGCGCTCTCCAGTCCT146260.14887123116894962No Hit
TCGTTTCCCGGGCGGCGCACCA146140.14874908876678722No Hit
AATGCACCCGGGCAAGGATTCT143090.1456446360451593No Hit
AGCTACATCTGGCTACTGGGTCTC142230.14476928216299537No Hit
TTCAAGTAATCCAGGATAGGCTT138100.14056554782190578No Hit
TTAATGCTAATCGTGATAGGGGTTT137680.14013804941433736No Hit
GGAATACCGGGTGCTGTAGGCTT131280.13362378796567556No Hit
TTCACAGTGGCTAAGTTCTGC130480.13280950528459282No Hit
AGCAGCATTGTACAGGGCTATGT127220.12949130335918071No Hit
AGCTACATTGTCTGCTGGGTTT127130.1293996965575589No Hit
TGATGTAGTAGGTTGTATAGTT124940.12717059771809494No Hit
TTCACCACCTTCTCCACCCAGC124650.12687542024620244No Hit
TGAGGTAGTAGGTTGTATAGT122120.12430025126727831No Hit
CAAAGAATTCTCCTTTTGGGCTT121200.12336382618403317No Hit
TATTGCACTTGTCCCGGCCTGTAAA120310.12245793670132865No Hit
TGTCAGTTTGTCAAATACCCCAA119270.12139936921592111No Hit
TGTAAACATCCCCGACTGGAAGC114480.11652385166293829No Hit
TGAGAACTGAATTCCATGGGTTG113180.11520064230617884No Hit
ATCACATTGCCAGGGATTTCC112840.11485457216671868No Hit
GAATACCGGGTGCTGTAGGCTTT111830.11382654028185173No Hit
AATGACACGATCACTCCCGTTG110190.11215726078563214No Hit
AACATTCAACGCTGTCGGTGA109420.11137351370509002No Hit
CCCATAAAGTAGAAAGCACTA109270.111220835702387No Hit
TGAGGGGCAGAGAGCGAGACTTT108360.11029458915265541No Hit
AACATTCAACGCTGTCGGTGAGA108060.10998923314724939No Hit
TACGCCTGTCTGAGCGTCGCT107950.1098772692786005No Hit
CAAAGTGCTGTTCGTGCAGGT107300.10921566460022078No Hit
AACATTCATTGCTGTCGGTGGGT105980.1078720981764343No Hit
AGCAGCATTGTACAGGGCTATG105780.10766852750616362No Hit
CAAAGTGCTGTTCGTGCAGGTAG105560.10744459976886585No Hit
TGTCAGTTTGTCAAATACCCCA103890.10574478467210567No Hit
TTCACAGTGGCTAAGTTCTG102900.10473710985426578No Hit
TATTGCACTTGTCCCGGCCTGA102820.10465568158615753No Hit
ACTGGACTTGGAGTCAGAAGGCT102040.10386175597210186No Hit
AGAGGTAGTAGGTTGCATAGTT101890.10370907796939885No Hit
TATTGCACTTGTCCCGGCCTGTAAT99430.10120515872506947No Hit
GGGAATACCGGGTGCTGTAGGCTTT98840.10060462524777096No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)