FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10309672
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG118277611.47248913447489No Hit
GCCTGTCTGAGCGTCGCT6829646.624497850174089No Hit
CCCCCCACTGCTAAATTTGACTG4824724.679799706528006No Hit
TAGCAGCACGTAAATATTGGCG4685014.544286180976466No Hit
TTCAAGTAATCCAGGATAGGCT4427014.294035736539436No Hit
AAGCTGCCAGTTGAAGAACTGT3119213.025518173614059No Hit
AACATTCAACGCTGTCGGTGAGT2488052.413316349928494No Hit
AACATTCAACGCTGTCGGTGAG2265672.197615986231182No Hit
CCCCCACTGCTAAATTTGACTG1848731.7931996284653866No Hit
TATTGCACTTGTCCCGGCCTGTAA1402901.360761040700422No Hit
CATAAAGTAGAAAGCACTACT1332521.2924950473691112No Hit
TAGCTTATCAGACTGATGTTGAC1307431.2681586766290915No Hit
TAGCTTATCAGACTGATGTTGA1167011.131956477373868No Hit
TGAGGTAGTAGGTTGTATAGTT1093181.0603441118204342No Hit
TATTGCACTTGTCCCGGCCTGTAT1071091.038917629969217No Hit
TAGCTTATCAGACTGATGTTGACT1038391.0071998410812681No Hit
TGTAAACATCCTTGACTGGAAGCT957450.9286910388613722No Hit
TATTGCACTTGTCCCGGCCTGT759040.7362406873855929No Hit
CCCATAAAGTAGAAAGCACTAC725970.704164012201358No Hit
TGAGGTAGTAGATTGTATAGTT658880.6390891970181011No Hit
CAACGGAATCCCAAAAGCAGCTGT644380.6250247340555548No Hit
TATTGCACTTGTCCCGGCCTGTA628270.6093986307226845No Hit
TTCAAGTAATTCAGGATAGGTT607060.5888257162788496No Hit
AGCAGCATTGTACAGGGCTATGA565270.5482909640578284No Hit
AACCGGGCGGAAACACCA557740.5409871429469337No Hit
CCCCCACTGCTAAATTTGACTGGCTT503310.48819205887442396No Hit
TGTAAACATCCCCGACTGGAAGCT428910.41602681443211775No Hit
ACTGGACTTGGAGTCAGAAGGC393240.381428235544254No Hit
CCCATAAAGTAGAAAGCACT388880.37719919702586074No Hit
TTCTCACTACTGCACTTGACTAG372210.36102991443374727No Hit
TGTAAACATCCTACACTCTCAGCT353900.34326989258242163No Hit
CATTGCACTTGTCTCGGTCTGA347850.33740161665666957No Hit
CTCCCACTGCTTCACTTGACTAG341860.3315915385086936No Hit
CACCCGGCTGTGTGCACATGTGC338010.32785718110139683No Hit
TAGCAGCACGTAAATATTGGC331120.32117413628678004No Hit
CAACGGAATCCCAAAAGCAGCT329970.32005867887940564No Hit
TCGTACGACTCTTAGCGG319680.3100777599908125No Hit
GGAATACCGGGTGCTGTAGGCTTT296500.2875940185099972No Hit
AACGGAATCCCAAAAGCAGCTG295690.28680834851002046No Hit
TCCTGTCTGAGCGTCGCT295170.2863039677692947No Hit
CAAAGAATTCTCCTTTTGGGCT287910.2792620366583923No Hit
TTCACAGTGGCTAAGTTCCG256450.2487470018444816No Hit
TGAGGTAGTAGTTTGTACAGTT254900.2472435592519335No Hit
CTTCTCACTACTGCACTTGACTAG248860.2413849829558108No Hit
TTCAAGTAATCCAGGATAGGC242590.23530331517821323No Hit
TAGCTTATCAGACTGATGTTGACA229630.22273259517858573No Hit
TGTCAGTTTGTCAAATACCCCAA208700.2024312703643724No Hit
CAACACCAGTCGATGGGCTGT207840.201597102216249No Hit
GGAATACCGGGTGCTGTAGGCTT206090.19989966703111406No Hit
CCCATAAAGTAGAAAGCACTA203490.19737776332748508No Hit
TGTCAGTTTGTCAAATACCCCA201040.19500135406829627No Hit
CCCCCCACTGCTAAATTTGACTGGCT197190.1912669966609995No Hit
CTACGCCTGTCTGAGCGTCGCT189830.1841280692538036No Hit
TTCACAGTGGCTAAGTTCCGC187240.181615865179804No Hit
TCGTTTCCCGGCCAATGCACCA184930.1793752507354259No Hit
AGCTCGGTCTGAGGCCCCTCAGT183140.17763901703177365No Hit
TGAGGTAGTAGTTTGTGCTGTT172840.16764839851355118No Hit
AACATTCAACGCTGTCGGTGAGTT170710.16558237740250126No Hit
CGCCTGTCTGAGCGTCGCT170490.16536898555065574No Hit
TTCACAGTGGCTAAGTTCTGC166130.1611399470322625No Hit
ATCACATTGCCAGGGATTTCC162260.15738619036570708No Hit
GTCAGGATGGCCGAGCGGTCTAAGG161430.15658112110647168No Hit
ACCCCCCACTGCTAAATTTGACTG158720.15395252147691993No Hit
TGTAAACATCCCCGACTGGAAGC156800.1520901925880862No Hit
TATTGCACTTGTCCCGGCCTGTAG151680.147123982217863No Hit
CAAAGAATTCTCCTTTTGGGCTT151060.14652260518084378No Hit
TAGCAGCACGTAAATATTGGCGT149210.14472817369941546No Hit
CACTAGATTGTGAGCTCCTGGA148460.1440007014772148No Hit
AGAATTACTCTGAGACCT146480.14208017481060503No Hit
CACTAGATTGTGAGCTCCTGGAA144670.14032454184769408No Hit
TATTGCACTTGTCCCGGCCTGTT142890.13859800777367118No Hit
TCAGTGCACTACAGAACTTTGT139380.13519343777377205No Hit
TGTAAACATCCTACACTCAGCT138300.1341458777738031No Hit
TTCAAGTAATCCAGGATAGGCTT138180.13402948221825098No Hit
CCCCCCACTGCTAAATTTGACTGG132480.1285006933295259No Hit
TAGCAGCACATCATGGTTTACA125980.12219593407045345No Hit
ACTGGACTTGGAGTCAGAAGGCA124990.12123567073714858No Hit
TTCACAGTGGCTAAGTTCTG120860.11722972370023022No Hit
TGATGTAGTAGGTTGTATAGTT118270.1147175196262306No Hit
TGAGGTAGTAGGTTGTGTGGTT117650.11411614258921138No Hit
ATCACATTGCCAGGGATTTCCA114730.11128385073744344No Hit
TATTGCACTTGTCCCGGCCTGTAAA114680.11123535258929673No Hit
CTCCCACTGCTTCACTTGACTAGCCT113970.11054667888561343No Hit
AGCAGCATTGTACAGGGCTATGT113470.11006169740414631No Hit
AATGACACGATCACTCCCGTTG112620.10923722888565224No Hit
ACTGGACTTGGAGTCAGAAGGCT111650.10829636481160604No Hit
TGTAAACATCCCCGACTGGA107580.10434861555246373No Hit
TTATAAAGCAATGAGACTGATT106900.10368904073766846No Hit
ACTGGACTTGGAGTCAGAAGG106150.1029615685154678No Hit
TGTCAGTTTGTCAAATACCCC103730.10061425814516699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)