FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5667528
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4611058.135910400442663No Hit
CCCCCCACTGCTAAATTTGACTG3442586.074217895350495No Hit
AACATTCAACGCTGTCGGTGAGT2284124.03018741151345No Hit
AAGCTGCCAGTTGAAGAACTGT2118563.7380671079172436No Hit
TAGCAGCACGTAAATATTGGCG1867103.294381606936922No Hit
TTCAAGTAATCCAGGATAGGCT1741753.073209342768134No Hit
AACATTCAACGCTGTCGGTGAG1500052.646744753620979No Hit
CCCCCACTGCTAAATTTGACTG1289642.275489419725849No Hit
TAGCTTATCAGACTGATGTTGA926931.6355102259750633No Hit
CGCCTGTCTGAGCGTCGCT821861.4501207581153548No Hit
TGAGGTAGTAGGTTGTATAGTT805791.4217662444720167No Hit
TAGCTTATCAGACTGATGTTGAC793591.4002401046805593No Hit
TAGCTTATCAGACTGATGTTGACT683741.2064166246730497No Hit
TATTGCACTTGTCCCGGCCTGT629481.1106782357316982No Hit
TATTGCACTTGTCCCGGCCTGTAA566821.0001185702126218No Hit
CATAAAGTAGAAAGCACTACT562850.993113752591959No Hit
TATTGCACTTGTCCCGGCCTGTAT530640.9362812146671353No Hit
TGAGGTAGTAGATTGTATAGTT414580.7315005766182363No Hit
TGTAAACATCCTTGACTGGAAGCT359750.634756458194825No Hit
AGCAGCATTGTACAGGGCTATGA344890.6085369141537544No Hit
TCGTACGACTCTTAGCGG334620.5904161390997981No Hit
TTCAAGTAATTCAGGATAGGTT328490.5796001360734344No Hit
TATTGCACTTGTCCCGGCCTGTA327430.5777298321243406No Hit
ACTGGACTTGGAGTCAGAAGGC313430.5530277044947992No Hit
CAACACCAGTCGATGGGCTGT281710.4970597410370094No Hit
CCCATAAAGTAGAAAGCACT269660.4757982668987255No Hit
CATTGCACTTGTCTCGGTCTGA265990.46932278058441No Hit
CAACGGAATCCCAAAAGCAGCTGT226810.40019211197545035No Hit
ACCGGGTGCTGTAGGCTT218260.3851061697445518No Hit
CCCATAAAGTAGAAAGCACTAC217000.382882978257893No Hit
TGTAAACATCCCCGACTGGAAGCT210930.37217284149279894No Hit
CAACGGAATCCCAAAAGCAGCT195730.34535338863786824No Hit
TGAGGTAGTAGTTTGTACAGTT193810.3419656682772454No Hit
TACGCCTGTCTGAGCGTCGCT169760.29953094188506874No Hit
ACGCCTGTCTGAGCGTCGCT157370.27766955893292455No Hit
TTCTCACTACTGCACTTGACTAG154240.2721468689700342No Hit
AACATTCAACGCTGTCGGTGAGTT149730.2641892549979462No Hit
CTACGCCTGTCTGAGCGTCGCT147560.2603604252153673No Hit
GCGTTCGCGCTTTCCCCTG145030.25589639786517154No Hit
TGTCAGTTTGTCAAATACCCCAA144820.2555258659507284No Hit
CTCCCACTGCTTCACTTGACTAG136350.24058107873485582No Hit
TGAGGTAGTAGTTTGTGCTGTT135210.238569619770736No Hit
GCCTGTCTGAGCGTCGCT129570.22861819121140645No Hit
ACCGGGTGCTGTAGGCTTA125630.22166630672137833No Hit
ACCGGGTGCTGTAGGCTTT125500.22143692982196117No Hit
AACGGAATCCCAAAAGCAGCTG125430.22131341918381348No Hit
CTTCTCACTACTGCACTTGACTAG120490.212597097005961No Hit
TAGTGGGTTATCAGAACT112720.19888741617156547No Hit
TTATAAAGCAATGAGACTGATT108670.19174144353587666No Hit
CACCCGGCTGTGTGCACATGTGC105990.1870127505325073No Hit
ATCCCACCGCTGCCACCA104940.18516009096029168No Hit
CACTAGATTGTGAGCTCCTGGA103240.18216054689099023No Hit
ATCCCGGACGAGCCCCCA102180.18029024294189636No Hit
TTCACAGTGGCTAAGTTCCGC97400.1718562307940958No Hit
TTCACAGTGGCTAAGTTCCG96800.17079756818140113No Hit
TGTAAACATCCTACACTCTCAGCT96060.16949188429241108No Hit
TAGCAGCACGTAAATATTGGC95650.1687684648404031No Hit
ACTGGACTTGGAGTCAGAAGG93730.16538074447978024No Hit
TATTGCACTTGTCCCGGCCTGTT93620.16518665633411955No Hit
TTCAAGTAATCCAGGATAGGCTT93560.16508079007285012No Hit
CGCGACCTCAGATCAGACGTGG92500.16321048612375624No Hit
ACCACAGGGTAGAACCACGGAC91220.16095200588334102No Hit
TTCAAGTAATCCAGGATAGGC90290.15931107883366433No Hit
TGAGGTAGTAGGTTGTGTGGTT88800.156682066678806No Hit
CCCCCCACTGCTAAATTTGACTGG87770.15486469586034687No Hit
TTCACAGTGGCTAAGTTCTGC86710.15299439191125302No Hit
TTCACAGTGGCTAAGTTCTG85180.1502948022488817No Hit
TGATGTAGTAGGTTGTATAGTT84100.14838920954603135No Hit
ACTGGACTTGGAGTCAGAAGGCT78160.13790844968035448No Hit
TGAGGTAGTAGGTTGTATAGT77110.13605579010813887No Hit
TAGCTTATCAGACTGATGTTGACA75510.13323268980761982No Hit
AGCAGCATTGTACAGGGCTATG75370.13298566853132443No Hit
ACTGGACTTGGAGTCAGAAGGCA73980.13053310014524852No Hit
TGTCAGTTTGTCAAATACCCCA72600.12809817613605085No Hit
CAAAGTGCTGTTCGTGCAGGT72590.1280805317591726No Hit
AGCAGCATTGTACAGGGCTATGT70320.12407525820781123No Hit
TGTAAACATCCCCGACTGGAAGC69560.12273428556506469No Hit
CACTAGATTGTGAGCTCCTGGAA65520.11560595730625416No Hit
TCGTTCGCGCTTTCCCCTG65140.11493547098488088No Hit
TATTGCACTTGTCCCGGCCTGTAG64510.11382387524155153No Hit
TCGTTTCCCGGCCAATGCACCA63610.11223588132250957No Hit
AATGACACGATCACTCCCGTTG62050.10948335852950353No Hit
TACCACCCTGAACGCGCCCGA61820.10907753786130392No Hit
ACCCCCCACTGCTAAATTTGACTG59840.10558395123941161No Hit
CCCATAAAGTAGAAAGCACTA59810.10553101810877688No Hit
AACATTCAACGCTGTCGGTGA59630.10521341932496849No Hit
TAGCAGCACGTAAATATTGGCGT58780.10371364729031775No Hit
TGAGGTAGTAAGTTGTATTGTT58070.10246089653196244No Hit
CAAAGAATTCTCCTTTTGGGCT57860.1020903646175193No Hit
TAGCTTATCAGACTGATGTTG57630.1016845439493197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)