FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7588516
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6076578.007586727101847No Hit
CCCCCCACTGCTAAATTTGACTG5159346.798878726749737No Hit
TTCAAGTAATCCAGGATAGGCT2547253.3567169127666068No Hit
TAGCAGCACGTAAATATTGGCG2415723.183389216020629No Hit
AACATTCAACGCTGTCGGTGAGT2340523.0842921066516826No Hit
AAGCTGCCAGTTGAAGAACTGT2207312.9087505383134196No Hit
AACATTCAACGCTGTCGGTGAG1552962.04646073092552No Hit
CCCCCACTGCTAAATTTGACTG1540152.0295799600343467No Hit
TAGCTTATCAGACTGATGTTGACT1422441.8744639926963322No Hit
TATTGCACTTGTCCCGGCCTGTAT1409461.8573591990845115No Hit
TATTGCACTTGTCCCGGCCTGTAA1387831.8288556023338423No Hit
TAGCTTATCAGACTGATGTTGA1324831.7458354176231559No Hit
TAGCTTATCAGACTGATGTTGAC1261631.6625516767705306No Hit
TGAGGTAGTAGGTTGTATAGTT839581.1063823282444156No Hit
TGTAAACATCCTTGACTGGAAGCT747870.9855286593584306No Hit
TATTGCACTTGTCCCGGCCTGT707010.9316841395603567No Hit
CATAAAGTAGAAAGCACTACT673070.8869586622733615No Hit
TTCAAGTAATTCAGGATAGGTT644640.8494941566967771No Hit
TGAGGTAGTAGATTGTATAGTT626460.8255369033945504No Hit
TCGTACGACTCTTAGCGG613060.8078786418846583No Hit
TATTGCACTTGTCCCGGCCTGTA587940.7747759904571592No Hit
AGCAGCATTGTACAGGGCTATGA512600.6754943917888557No Hit
TGTAAACATCCCCGACTGGAAGCT424280.5591079995087314No Hit
TTAATGCTAATCGTGATAGGGGTT393440.5184676424217858No Hit
CAACGGAATCCCAAAAGCAGCTGT384960.5072928620035855No Hit
ACTGGACTTGGAGTCAGAAGGC348010.45860086478041295No Hit
CCCATAAAGTAGAAAGCACTAC339680.44762375146866656No Hit
TTCTCACTACTGCACTTGACTAG321370.4234951866741798No Hit
GCCTGTCTGAGCGTCGCT317010.4177496627799164No Hit
CTTCTCACTACTGCACTTGACTAG261710.34487638953386934No Hit
TGAGGTAGTAGTTTGTACAGTT252650.3329372963040468No Hit
CATTGCACTTGTCTCGGTCTGA252300.3324760730556541No Hit
CAACGGAATCCCAAAAGCAGCT242130.3190742432380718No Hit
CCCATAAAGTAGAAAGCACT240770.3172820614728888No Hit
CTCCCACTGCTTCACTTGACTAG235090.3097970670418301No Hit
CCCCCACTGCTAAATTTGACTGGCTT220880.2910714031570863No Hit
CAAAGAATTCTCCTTTTGGGCT216970.28591888058218495No Hit
TAGCTTATCAGACTGATGTTGACA194280.2560184362792409No Hit
TGTAAACATCCTACACTCTCAGCT178420.23511843422350298No Hit
TGAGAACTGAATTCCATGGGTT174750.23028217901892808No Hit
CACTAGATTGTGAGCTCCTGGA171640.22618388101178147No Hit
CACTAGATTGTGAGCTCCTGGAA167410.22060966860977826No Hit
AACATTCAACGCTGTCGGTGAGTT166190.21900197614395225No Hit
TATTGCACTTGTCCCGGCCTGTAG163440.21537807919229532No Hit
TAGCAGCACGTAAATATTGGC159080.2096325552980319No Hit
TGATGTAGTAGGTTGTATAGTT156750.2065621262444462No Hit
CAACACCAGTCGATGGGCTGT153440.20220027209536096No Hit
TGAGGTAGTAGTTTGTGCTGTT151030.19902442058499975No Hit
CCCCCCACTGCTAAATTTGACTGGCT150220.19795701821014805No Hit
TATTGCACTTGTCCCGGCCTGTT147600.19450443275075127No Hit
TTCAAGTAATCCAGGATAGGCTT147030.19375329774622602No Hit
ACCCCCCACTGCTAAATTTGACTG144030.1897999556171457No Hit
CAAAGAATTCTCCTTTTGGGCTT143320.18886433131326336No Hit
CGCCTGTCTGAGCGTCGCT140830.18558305734612668No Hit
TACGCCTGTCTGAGCGTCGCT137790.18157700398865867No Hit
CTCCCACTGCTTCACTTGACTAGCCT135700.17882284230539938No Hit
TTATAAAGCAATGAGACTGATT134910.17778179554474155No Hit
TGTAAACATCCCCGACTGGAAGC134540.17729421668215498No Hit
TCCTGTCTGAGCGTCGCT130450.17190449357950882No Hit
CCCCCCACTGCTAAATTTGACTGG125480.16535512345233244No Hit
TCGTTTCCCGGCCAATGCACCA123330.16252189492649155No Hit
TATTGCACTTGTCCCGGCCTGTTT123080.16219244974906818No Hit
CACCCGGCTGTGTGCACATGTGC122660.16163898185099695No Hit
TATTGCACTTGTCCCGGCCTGTAAT120070.15822592981289096No Hit
AGCAGCATTGTACAGGGCTATGT118170.15572214646447344No Hit
AACGGAATCCCAAAAGCAGCTG118120.15565625742898875No Hit
TTCAAGTAATCCAGGATAGGC116260.15320518530895896No Hit
TGATGTAGTAGATTGTATAGTT116160.15307340723798962No Hit
TGTCAGTTTGTCAAATACCCCAA112410.14813172957663923No Hit
ACTGGACTTGGAGTCAGAAGGCT112030.14763097290695573No Hit
CTACGCCTGTCTGAGCGTCGCT110900.14614188070500214No Hit
TAGTGGGTTATCAGAACT109450.14423109867594666No Hit
ACCGGGTGCTGTAGGCTTA109260.1439807203411049No Hit
CCCCCCACTGCTAAATTTGACTGTA106160.13989560014105526No Hit
GGAATACCGGGTGCTGTAGGCTTT106000.13968475522750431No Hit
TGTCAGTTTGTCAAATACCCCA104750.13803752934038752No Hit
ATCCCGGACGAGCCCCCA104400.1375763060919948No Hit
ACTGGACTTGGAGTCAGAAGGCA103830.13682517108746955No Hit
AGCTCGGTCTGAGGCCCCTCAGT102030.13445316581002137No Hit
TTCACAGTGGCTAAGTTCCG102010.13442681019582747No Hit
ACCGGGTGCTGTAGGCTT100190.13202844930418542No Hit
CCCCCCACTGCTAAATTTGACTGT98600.12993317797577286No Hit
TCGTTTCCCGGGCGGCGCACCA97350.1282859520886561No Hit
TTCACAGTGGCTAAGTTCCGC94090.12398998697505546No Hit
TTCACAGTGGCTAAGTTCTGC87190.11489730007817076No Hit
ATGCACCTGGGCAAGGATTCTGA87160.11485776665687995No Hit
CGTTCGCGCTTTCCCCTG86870.11447561025106885No Hit
TAGCAGCACGTAAATATTGGCGT86710.11426476533751789No Hit
CCCATAAAGTAGAAAGCACTA86580.11409345384525776No Hit
TATTGCACTTGTCCCGGCCTGTAAA83840.11048273470069775No Hit
GGAATACCGGGTGCTGTAGGCTT82360.10853241925035145No Hit
TCAGTGCACTACAGAACTTTGT77600.10225978307221069No Hit
TTCACAGTGGCTAAGTTCTG77210.10174584859543026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)