FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10403944
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8154347.837739226585611No Hit
CCCCCCACTGCTAAATTTGACTG7911797.604606483848817No Hit
AACATTCAACGCTGTCGGTGAGT3600463.4606683772999935No Hit
TTCAAGTAATCCAGGATAGGCT3375913.2448367657496044No Hit
AAGCTGCCAGTTGAAGAACTGT3287543.1598978233639086No Hit
TAGCAGCACGTAAATATTGGCG3106882.9862521366897017No Hit
CCCCCACTGCTAAATTTGACTG2811402.702244456525333No Hit
AACATTCAACGCTGTCGGTGAG2505122.4078560976491223No Hit
TAGCTTATCAGACTGATGTTGACT1924541.8498177229712118No Hit
TATTGCACTTGTCCCGGCCTGTAA1900461.8266726541396223No Hit
TATTGCACTTGTCCCGGCCTGTAT1869701.7971069432899678No Hit
TAGCTTATCAGACTGATGTTGAC1749571.6816411160998177No Hit
TAGCTTATCAGACTGATGTTGA1620611.5576881228887813No Hit
TCGTACGACTCTTAGCGG1576181.5149831640770077No Hit
TGAGGTAGTAGGTTGTATAGTT1104961.062058773096049No Hit
TATTGCACTTGTCCCGGCCTGT1016170.9767161376493376No Hit
TGTAAACATCCTTGACTGGAAGCT1002910.9639709710086868No Hit
TGAGGTAGTAGATTGTATAGTT845690.8128552018349965No Hit
GCCTGTCTGAGCGTCGCT817390.7856539789141503No Hit
TTCAAGTAATTCAGGATAGGTT804550.7733125053345155No Hit
TATTGCACTTGTCCCGGCCTGTA765820.736086238065103No Hit
CATAAAGTAGAAAGCACTACT751670.72248562660468No Hit
TTAATGCTAATCGTGATAGGGGTT709330.6817895213584387No Hit
AGCAGCATTGTACAGGGCTATGA682000.6555206371737488No Hit
TGTAAACATCCCCGACTGGAAGCT615550.5916506278772742No Hit
CAACGGAATCCCAAAAGCAGCTGT513930.49397613059047607No Hit
CCCATAAAGTAGAAAGCACTAC489390.4703889217396787No Hit
ACTGGACTTGGAGTCAGAAGGC459240.4414095270024522No Hit
CCCATAAAGTAGAAAGCACT441970.4248100528030524No Hit
TTCTCACTACTGCACTTGACTAG431100.414362091914374No Hit
CATTGCACTTGTCTCGGTCTGA378750.3640446353805826No Hit
TGAGGTAGTAGTTTGTACAGTT361960.3479065246794869No Hit
CTTCTCACTACTGCACTTGACTAG355910.3420914222529456No Hit
CAACGGAATCCCAAAAGCAGCT353100.339390523439957No Hit
TGAGAACTGAATTCCATGGGTT351230.33759312814448056No Hit
TGTAAACATCCTACACTCTCAGCT319600.30719119595414973No Hit
TCCTGTCTGAGCGTCGCT307800.2958493432875071No Hit
CTCCCACTGCTTCACTTGACTAG298530.2869392607265091No Hit
CAAAGAATTCTCCTTTTGGGCT262570.25237544531189327No Hit
TAGCTTATCAGACTGATGTTGACA239280.22998970390459617No Hit
AACATTCAACGCTGTCGGTGAGTT238770.22949950518764808No Hit
TGTCAGTTTGTCAAATACCCCAA221770.21315954795604436No Hit
CGCCTGTCTGAGCGTCGCT217990.2095263104068995No Hit
TATTGCACTTGTCCCGGCCTGTAG216980.2085555247125513No Hit
CCCCCACTGCTAAATTTGACTGGCTT216450.20804610251650718No Hit
CAACACCAGTCGATGGGCTGT214390.20606608416961875No Hit
TGATGTAGTAGGTTGTATAGTT211650.20343246753346617No Hit
TAGCAGCACGTAAATATTGGC205710.19772309424195286No Hit
CACTAGATTGTGAGCTCCTGGA205390.1974155185764168No Hit
TATTGCACTTGTCCCGGCCTGTT202930.19505103064760826No Hit
CACTAGATTGTGAGCTCCTGGAA201410.19359004623631193No Hit
ATCCCGGACGAGCCCCCA199320.19158119267077947No Hit
TTCAAGTAATCCAGGATAGGCTT199270.19153313397303945No Hit
GGAATACCGGGTGCTGTAGGCTTT194880.1873135803114665No Hit
TACGCCTGTCTGAGCGTCGCT192090.1846319049775739No Hit
TCGTTTCCCGGCCAATGCACCA190660.18325742622220959No Hit
TGTAAACATCCCCGACTGGAAGC188260.18095060873068905No Hit
TGAGGTAGTAGTTTGTGCTGTT184550.17738465335838025No Hit
CAAAGAATTCTCCTTTTGGGCTT181910.17484715411770765No Hit
ACCGGGTGCTGTAGGCTTA175910.16908011038890636No Hit
AACGGAATCCCAAAAGCAGCTG175910.16908011038890636No Hit
TTATAAAGCAATGAGACTGATT172580.16587940111942165No Hit
TGTCAGTTTGTCAAATACCCCA172050.16536997892337751No Hit
AGCTCGGTCTGAGGCCCCTCAGT169000.16243839836123686No Hit
ACCCCCCACTGCTAAATTTGACTG166600.16013158086971632No Hit
CACCCGGCTGTGTGCACATGTGC165360.15893972516576407No Hit
ACCGGGTGCTGTAGGCTT164050.15768058728497578No Hit
TGATGTAGTAGATTGTATAGTT161750.15546988718893529No Hit
CCCCCCACTGCTAAATTTGACTGG160370.15414346713131097No Hit
TATTGCACTTGTCCCGGCCTGTTT158130.1519904374725585No Hit
TTCAAGTAATCCAGGATAGGC157610.1514906270160624No Hit
ACTGGACTTGGAGTCAGAAGGCT156830.1507409113313182No Hit
TATTGCACTTGTCCCGGCCTGTAAT154670.14866477558894972No Hit
GGAATACCGGGTGCTGTAGGCTT154350.14835719992341365No Hit
AGCAGCATTGTACAGGGCTATGT151740.14584853590138508No Hit
CCCCCCACTGCTAAATTTGACTGGCT147410.14168665267710015No Hit
CTCCCACTGCTTCACTTGACTAGCCT140720.13525639891948668No Hit
ACTGGACTTGGAGTCAGAAGGCA140600.13514105804491067No Hit
CTACGCCTGTCTGAGCGTCGCT140010.13457396541157854No Hit
TGAGAACTGAATTCCATAGGCTGT139800.13437211888107048No Hit
TTCACAGTGGCTAAGTTCCG130730.12565427111103253No Hit
CCCATAAAGTAGAAAGCACTA128000.1230302662144279No Hit
CGTTCGCGCTTTCCCCTG126020.12112714178392349No Hit
AACCGGGCGGAAACACCA125100.12024286174550727No Hit
TATTGCACTTGTCCCGGCCTGTAAA123180.11839740775229086No Hit
TTCACAGTGGCTAAGTTCCGC122710.11794565599353476No Hit
TCGTTTCCCGGGCGGCGCACCA121970.11723438726698258No Hit
TCAGTGCACTACAGAACTTTGT120990.11629243679127839No Hit
AACATTCAACGCTGTCGGTGA118370.11377416102970181No Hit
CCCCCCACTGCTAAATTTGACTGT115110.1106407339370531No Hit
ACCGGGTGCTGTAGGCTTT113900.10947771345174483No Hit
TTCACAGTGGCTAAGTTCTGC110860.10655574462915217No Hit
ATGCACCTGGGCAAGGATTCTGA108540.10432582105401568No Hit
CCCCCCACTGCTAAATTTGACTGTA104540.1004811252348148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)