FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8956133
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7885888.805005463853652No Hit
CAACGGAATCCCAAAAGCAGCTG6436037.1861706386003865No Hit
AACATTCAACGCTGTCGGTGAGT4124624.605358138384054No Hit
TTCAAGTAATCCAGGATAGGCT2932243.2740022954103067No Hit
AAGCTGCCAGTTGAAGAACTGT2886183.2225738496737377No Hit
CCCCCACTGCTAAATTTGACTG2572372.8721882535688117No Hit
TAGCAGCACGTAAATATTGGCG2528022.822669114002662No Hit
AACATTCAACGCTGTCGGTGAG2368482.6445341979624466No Hit
TATTGCACTTGTCCCGGCCTGTAA1470331.6417018371656606No Hit
TATTGCACTTGTCCCGGCCTGTAT1451351.6205096552273175No Hit
TAGCTTATCAGACTGATGTTGA1282841.4323592559422689No Hit
TAGCTTATCAGACTGATGTTGACT1262691.4098607066241646No Hit
TAGCTTATCAGACTGATGTTGAC1195291.3346050131234095No Hit
TGAGGTAGTAGGTTGTATAGTT999891.1164304951701811No Hit
TATTGCACTTGTCCCGGCCTGT967041.0797517187384331No Hit
TATTGCACTTGTCCCGGCCTGTA798580.8916571471191863No Hit
CATAAAGTAGAAAGCACTACT739070.8252110592819467No Hit
TTAATGCTAATCGTGATAGGGGTT692050.7727107223619837No Hit
TGAGGTAGTAGATTGTATAGTT691780.7724092529666543No Hit
GCCTGTCTGAGCGTCGCT681000.7603728082197976No Hit
TGTAAACATCCTTGACTGGAAGCT633960.7078501402335138No Hit
TCGTACGACTCTTAGCGG554500.619128813741377No Hit
AGCAGCATTGTACAGGGCTATGA540590.6035975571153309No Hit
TTCAAGTAATTCAGGATAGGTT518110.5784974385708653No Hit
CCCATAAAGTAGAAAGCACTAC466780.5211847568587916No Hit
ACTGGACTTGGAGTCAGAAGGC435860.4866609283269911No Hit
CAACGGAATCCCAAAAGCAGCTGT400380.4470456166740713No Hit
TGTAAACATCCCCGACTGGAAGCT393960.4398773443851269No Hit
CCCATAAAGTAGAAAGCACT365780.40841287193926223No Hit
TTCTCACTACTGCACTTGACTAG330960.3695344854749254No Hit
AACATTCAACGCTGTCGGTGAGTT314460.3511113557603488No Hit
CAACGGAATCCCAAAAGCAGCT307260.3430721718848972No Hit
CATTGCACTTGTCTCGGTCTGA304650.34015796773004603No Hit
CTCCCACTGCTTCACTTGACTAG288510.322136797209242No Hit
CGCCTGTCTGAGCGTCGCT287870.3214222030869796No Hit
TGAGGTAGTAGTTTGTACAGTT280410.31309271534935895No Hit
CTTCTCACTACTGCACTTGACTAG275410.3075099487691842No Hit
ACCGGGTGCTGTAGGCTTA265990.29699201653213503No Hit
TCCTGTCTGAGCGTCGCT254510.28417398446405384No Hit
ACCGGGTGCTGTAGGCTT253890.2834817214081122No Hit
ATCCCGGACGAGCCCCCA239010.26686740806551223No Hit
CAACACCAGTCGATGGGCTGT219590.24518394266811355No Hit
TTCACAGTGGCTAAGTTCCG209750.23419705803832971No Hit
TGTAAACATCCTACACTCTCAGCT207170.23131635048295957No Hit
TATTGCACTTGTCCCGGCCTGTT193420.21596374238747906No Hit
CACTAGATTGTGAGCTCCTGGA187510.20936491228971252No Hit
CCCCCCACTGCTAAATTTGACTGG183680.2050885130892987No Hit
ACCGGGTGCTGTAGGCTTT182240.20348067631420838No Hit
TATTGCACTTGTCCCGGCCTGTAG177740.19845618639205112No Hit
TGTCAGTTTGTCAAATACCCCAA177530.1982217101956838No Hit
CCCCCACTGCTAAATTTGACTGGCTT176910.19752944713974213No Hit
CAAAGAATTCTCCTTTTGGGCT176600.1971833156117713No Hit
TGAGGTAGTAGTTTGTGCTGTT169000.1886975104099057No Hit
CCCCCCACTGCTAAATTTGACTGGCT163700.1827797778349205No Hit
GGAATACCGGGTGCTGTAGGCTTT162840.18181954198313044No Hit
CACTAGATTGTGAGCTCCTGGAA158540.17701836272418017No Hit
TGATGTAGTAGGTTGTATAGTT150010.16749416293840208No Hit
AACGGAATCCCAAAAGCAGCTG147550.16474744178095613No Hit
TAGCTTATCAGACTGATGTTGACA147150.16430082045454214No Hit
CACCCGGCTGTGTGCACATGTGC144140.16093999497327696No Hit
TCGTTTCCCGGCCAATGCACCA142810.1594549790629505No Hit
TTCAAGTAATCCAGGATAGGC142790.1594326479966298No Hit
ACCCCCCACTGCTAAATTTGACTG140920.15734469329564446No Hit
ACTGGACTTGGAGTCAGAAGGCA140710.1571102170992771No Hit
TTCAAGTAATCCAGGATAGGCTT140350.15670825790550452No Hit
GGAATACCGGGTGCTGTAGGCTT137390.1534032600900411No Hit
TTATAAAGCAATGAGACTGATT134090.14971863414712577No Hit
CCCATAAAGTAGAAAGCACTA131670.14701657512232122No Hit
TAGCAGCACGTAAATATTGGC129900.14504027575293935No Hit
TTCACAGTGGCTAAGTTCTG129720.14483929615605307No Hit
AGCTCGGTCTGAGGCCCCTCAGT129710.14482813062289274No Hit
CTCCCACTGCTTCACTTGACTAGCCT126960.14175760900379664No Hit
TGAGAACTGAATTCCATGGGTT126960.14175760900379664No Hit
CTACGCCTGTCTGAGCGTCGCT126890.14167945027167417No Hit
ACTGGACTTGGAGTCAGAAGGCT124630.13915603977743518No Hit
AACATTCAACGCTGTCGGTGA121720.1359068696277735No Hit
TGAGGTAGTAGGTTGTGTGGTT117430.1311168559019836No Hit
TATTGCACTTGTCCCGGCCTGTTT115730.12921871526472417No Hit
CCCCCCACTGCTAAATTTGACTGT114570.12792351341812366No Hit
TGTAAACATCCCCGACTGGAAGC114210.12752155422435107No Hit
TGTCAGTTTGTCAAATACCCCA113630.1268739533010508No Hit
ACTGGACTTGGAGTCAGAAGG112440.12554525485496923No Hit
AACATTCAACGCTGTCGGTGAGA110530.12341263802134246No Hit
TGAGGTAGTAGGTTGTATAGT109750.12254172643483521No Hit
CCCCCCACTGCTAAATTTGACTGTA108890.12158149058304517No Hit
CAAAGAATTCTCCTTTTGGGCTT108310.1209338896597449No Hit
ACCACAGGGTAGAACCACGGAC107720.12027512320328428No Hit
AGCAGCATTGTACAGGGCTATGT106220.11860029322923187No Hit
TATTGCACTTGTCCCGGCCTGA103920.11603222060235148No Hit
TACGCCTGTCTGAGCGTCGCT102190.11410058336561103No Hit
TGATGTAGTAGATTGTATAGTT100860.11261556745528455No Hit
TTAATGCTAATCGTGATAGGGGT100380.11207962186358778No Hit
TTCACAGTGGCTAAGTTCTGC97950.10936639730562286No Hit
TATTGCACTTGTCCCGGCCTGTAAT97380.10872996191548295No Hit
CAAAGTGCTGTTCGTGCAGGT93850.10478852870987958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)