FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14667541
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG203071313.844945107022369No Hit
CAACGGAATCCCAAAAGCAGCTG9266956.317998361143153No Hit
GGAATACCGGGTGCTGTAGGCTTT5590453.811443240554092No Hit
CCCCCACTGCTAAATTTGACTG5400903.6822123081162688No Hit
AACATTCAACGCTGTCGGTGAGT5295753.6105233999345905No Hit
GGAATACCGGGTGCTGTAGGCTT4637043.1614297174966137No Hit
TAGCAGCACGTAAATATTGGCG3692922.5177499077725436No Hit
AACATTCAACGCTGTCGGTGAG3519552.3995501359089433No Hit
AAGCTGCCAGTTGAAGAACTGT3362602.2925451512288255No Hit
TTCAAGTAATCCAGGATAGGCT3124372.1301252882129322No Hit
TATTGCACTTGTCCCGGCCTGTAT2271301.5485213233765633No Hit
TATTGCACTTGTCCCGGCCTGTAA2121331.4462751459157333No Hit
TAGCTTATCAGACTGATGTTGACT1549341.056305211623407No Hit
TAGCTTATCAGACTGATGTTGA1460750.9959065394806124No Hit
TATTGCACTTGTCCCGGCCTGT1425420.9718193390425839No Hit
TAGCTTATCAGACTGATGTTGAC1394900.9510114885651249No Hit
TGAGGTAGTAGGTTGTATAGTT1383290.9430960513422121No Hit
CGCCTGTCTGAGCGTCGCT1171340.7985933020401988No Hit
TATTGCACTTGTCCCGGCCTGTA1128350.7692836856566483No Hit
GGAATACCGGGTGCTGTAGGCTTA1059870.7225955598146956No Hit
TCGTACGACTCTTAGCGG1009800.6884589584579992No Hit
TGAGGTAGTAGATTGTATAGTT984780.6714008844427297No Hit
AGCAGCATTGTACAGGGCTATGA862800.5882376602867515No Hit
GCCTGTCTGAGCGTCGCT828370.564764059633445No Hit
TGTAAACATCCTTGACTGGAAGCT796730.5431926183127764No Hit
ACTGGACTTGGAGTCAGAAGGC794350.5415699877709562No Hit
CATAAAGTAGAAAGCACTACT729080.49707036782784514No Hit
GGAATACCGGGTGCTGTAGGCT719530.49055939233440693No Hit
CTCCCACTGCTTCACTTGACTAG623830.425313281892309No Hit
TGTAAACATCCCCGACTGGAAGCT556870.37966145790899786No Hit
TTCAAGTAATTCAGGATAGGTT551280.37585032146833613No Hit
ACCGGGTGCTGTAGGCTT547440.37323229572018923No Hit
ACCGGGTGCTGTAGGCTTA535450.365057783032616No Hit
TTCTCACTACTGCACTTGACTAG495660.3379298547725212No Hit
CATTGCACTTGTCTCGGTCTGA476670.32498289931488855No Hit
CAACGGAATCCCAAAAGCAGCTGT467380.3186491859814811No Hit
CCCATAAAGTAGAAAGCACTAC446110.30414777773588636No Hit
CTTCTCACTACTGCACTTGACTAG439080.29935488163966956No Hit
ATCCCGGACGAGCCCCCA426220.2905872224935318No Hit
TGAGGTAGTAGTTTGTACAGTT399740.27253375327193563No Hit
CCCATAAAGTAGAAAGCACT394480.26894760341900525No Hit
CCCCCCACTGCTAAATTTGACTGG392090.2673181551017993No Hit
AACATTCAACGCTGTCGGTGAGTT374880.25558476366283894No Hit
CAACACCAGTCGATGGGCTGT357830.24396045663005136No Hit
ACCGGGTGCTGTAGGCTTT347980.23724494787503916No Hit
AGCTCGGTCTGAGGCCCCTCAGT343360.23409513564679996No Hit
TATTGCACTTGTCCCGGCCTGTAG304270.20744445166371106No Hit
TACGCCTGTCTGAGCGTCGCT285890.19491338050461218No Hit
TATTGCACTTGTCCCGGCCTGTT284020.19363845650746775No Hit
CACTAGATTGTGAGCTCCTGGA282400.1925339768949683No Hit
ACCCCCCACTGCTAAATTTGACTG275220.1876388141679645No Hit
CCCCCCACTGCTAAATTTGACTGGCT275010.1874956408848627No Hit
AACGGAATCCCAAAAGCAGCTG265940.18131191860994286No Hit
CACTAGATTGTGAGCTCCTGGAA262080.17868025731102438No Hit
TGAGGTAGTAGTTTGTGCTGTT254890.1737782768086348No Hit
CCCCCACTGCTAAATTTGACTGGCTT253610.1729056015592525No Hit
TGTAAACATCCTACACTCTCAGCT245180.16715821690902383No Hit
CTACGCCTGTCTGAGCGTCGCT239960.16359933815763666No Hit
ACTGGACTTGGAGTCAGAAGGCT220750.15050239164151646No Hit
TTCACAGTGGCTAAGTTCCG219650.14975243634907856No Hit
CACCCGGCTGTGTGCACATGTGC211940.1444959315266274No Hit
ACTGGACTTGGAGTCAGAAGGCA203630.13883036018102832No Hit
ACTGGACTTGGAGTCAGAAGG202660.13816903596860577No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC198860.13557828132200211No Hit
TAGCAGCACGTAAATATTGGC198680.1354555613650577No Hit
CTCCCACTGCTTCACTTGACTAGCCT197750.1348215082541784No Hit
CCCCCCACTGCTAAATTTGACTGA196700.1341056418386695No Hit
TGTCAGTTTGTCAAATACCCCAA195420.13323296658928718No Hit
AACCGGGCGGAAACACCA194680.13272845121073806No Hit
ACGCCTGTCTGAGCGTCGCT194460.13257846015225047No Hit
GGAATACCGGGTGCTGTAGGCTA181760.12391988541228553No Hit
TATTGCACTTGTCCCGGCCTGTTT181730.12389943208612814No Hit
TGAGGTAGTAGGTTGTGTGGTT180580.12311538791676124No Hit
AGCAGCATTGTACAGGGCTATGT176440.12029282890704039No Hit
TAGCTTATCAGACTGATGTTGACA175700.11978831352849124No Hit
TTCAAGTAATCCAGGATAGGCTT173700.11842475845133141No Hit
GTCAGGATGGCCGAGCGGTCTAAGG172740.1177702520142947No Hit
TTCAAGTAATCCAGGATAGGC166560.11355686682587081No Hit
CCCCCCACTGCTAAATTTGACTGT165900.11310689365040807No Hit
ATCCCACCGCTGCCACCA165210.11263646714878793No Hit
CGCGACCTCAGATCAGACGT162180.11057068120689077No Hit
TTCACAGTGGCTAAGTTCTG159910.10902304619431437No Hit
CAAAGAATTCTCCTTTTGGGCT159420.10868897520041022No Hit
TGTAAACATCCCCGACTGGAAGC157430.10733223789863619No Hit
GACCCGCGGGCGCTCTCCAGTCCT156400.10663000703389885No Hit
CTCGTACGACTCTTAGCGG155960.1063300249169237No Hit
TCGTTTCCCGGGCGGCGCACCA155930.1063095715907663No Hit
AGCAGCATTGTACAGGGCTATG155490.10600958947379115No Hit
CGCGACCTCAGATCAGACGTGGC154680.10545734966754142No Hit
TGAGGTAGTAGGTTGTATAGT153140.10440741225812833No Hit
TAGTGGGTTATCAGAACT151820.10350746590720285No Hit
ACCACAGGGTAGAACCACGGAC151220.1030983993840549No Hit
GCGTTCGCGCTTTCCCCTG150920.10289386612248094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)