FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9136790
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG92019710.071337964427332No Hit
AACATTCAACGCTGTCGGTGAGT3339743.6552662368293456No Hit
CCCCCCACTGCTAAATTTGACTG2925433.20181376610385No Hit
AAGCTGCCAGTTGAAGAACTGT2809593.0750296329454874No Hit
TAGCAGCACGTAAATATTGGCG2665012.9167902512808106No Hit
AACATTCAACGCTGTCGGTGAG2616732.863948936114325No Hit
TTCAAGTAATCCAGGATAGGCT2616052.86320469223874No Hit
TAGCTTATCAGACTGATGTTGACT2145622.3483302122517866No Hit
TAGCTTATCAGACTGATGTTGAC2118072.3181773905277456No Hit
TATTGCACTTGTCCCGGCCTGTAA1955572.140324993788847No Hit
TATTGCACTTGTCCCGGCCTGTAT1663351.8204971330193647No Hit
TAGCTTATCAGACTGATGTTGA1616341.7690458027381606No Hit
CCCCCACTGCTAAATTTGACTG1217331.3323388192133123No Hit
TATTGCACTTGTCCCGGCCTGT1057351.1572445027192262No Hit
TGAGGTAGTAGGTTGTATAGTT943421.032550819270225No Hit
TATTGCACTTGTCCCGGCCTGTA721530.7896974758093379No Hit
GCCTGTCTGAGCGTCGCT705860.77254703238227No Hit
TGTAAACATCCTTGACTGGAAGCT703780.770270521704012No Hit
AGCAGCATTGTACAGGGCTATGA634820.6947954369094617No Hit
CATAAAGTAGAAAGCACTACT627660.6869589866900738No Hit
TGAGGTAGTAGATTGTATAGTT626670.6858754551653261No Hit
TTCAAGTAATTCAGGATAGGTT606160.663427746506158No Hit
TGTAAACATCCCCGACTGGAAGCT561750.6148220545727766No Hit
CATTGCACTTGTCTCGGTCTGA497630.5446442350103264No Hit
ACTGGACTTGGAGTCAGAAGGC474860.5197230099411281No Hit
CAACGGAATCCCAAAAGCAGCTGT467910.5121163997421413No Hit
CAACGGAATCCCAAAAGCAGCT443520.4854221230869923No Hit
CACCCGGCTGTGTGCACATGTGC419380.4590014655037491No Hit
TCGTACGACTCTTAGCGG403190.44128189440711674No Hit
CCCATAAAGTAGAAAGCACT367260.4019573613927867No Hit
TGATGTAGTAGGTTGTATAGTT331060.36233731978079825No Hit
CAACACCAGTCGATGGGCTGT328880.359951361473778No Hit
CCCATAAAGTAGAAAGCACTAC319980.3502105225139245No Hit
TAGCTTATCAGACTGATGTTGACA274880.3008496419420825No Hit
TCCTGTCTGAGCGTCGCT267950.29326492126884823No Hit
CACTAGATTGTGAGCTCCTGGA252100.275917472110008No Hit
CGCGACCTCAGATCAGACGTGGCG230360.25212355761706245No Hit
TGATGTAGTAGATTGTATAGTT219840.24060966707125808No Hit
AACATTCAACGCTGTCGGTGAGTT217270.23779686301206443No Hit
CACTAGATTGTGAGCTCCTGGAA211750.23175535390438No Hit
TAGCAGCACGTAAATATTGGC209130.22888782603080515No Hit
CCCCCACTGCTAAATTTGACTGGCTT206030.22549494953917076No Hit
TGTAAACATCCTACACTCTCAGCT195170.2136089370555742No Hit
TGAGGTAGTAGTTTGTACAGTT194540.2129194169943711No Hit
CACCCGTAGAACCGACCTTGCG193390.21166076926360353No Hit
TATTGCACTTGTCCCGGCCTGTAG192160.210314563429826No Hit
TTCTCACTACTGCACTTGACTAG191890.21001905483216754No Hit
TATTGCACTTGTCCCGGCCTGTT191620.20972354623450903No Hit
CAAAGAATTCTCCTTTTGGGCT189230.20710774790708772No Hit
ACTTGACTTGGAGTCAGAAGGC188010.20577248683618646No Hit
TACCACCCTGAACGCGCCCGA187360.20506107724923084No Hit
CGCGACCTCAGATCAGACGTGG186240.20383526380709197No Hit
TATTGCACTTGTCCCGGCCTGTAAT182030.19922751863619498No Hit
TTCAAGTAATCCAGGATAGGCTT180280.19731218513285304No Hit
TTATAAAGCAATGAGACTGATT179820.19680872604054597No Hit
CTTCTCACTACTGCACTTGACTAG175430.19200397513787665No Hit
AACGGAATCCCAAAAGCAGCTG165400.18102637797300802No Hit
CAAAGAATTCTCCTTTTGGGCTT153460.16795833109877759No Hit
TACCACCCTGAACGCGCCCGAT150620.16485001844192543No Hit
CAACGGAATCCCAAAAGCAGCTGA142940.1564444405529732No Hit
TATTGCACTTGTCCCGGCCTGTAAA142390.15584247859478No Hit
CTCCCACTGCTTCACTTGACTAG141080.15440871465799258No Hit
AGCAGCATTGTACAGGGCTATGT139270.15242771257739315No Hit
TGAGGTAGTAGTTTGTGCTGTT138210.15126756771251174No Hit
ACTGGACTTGGAGTCAGAAGGCT138190.15124567818675924No Hit
TGCAAGTAATCCAGGATAGGCT134170.1468458835105108No Hit
AGCTCGGTCTGAGGCCCCTCAGT128650.1408043744028264No Hit
AATGCACCCGGGCAAGGATTCT124870.1366672540356077No Hit
TTCACAGTGGCTAAGTTCCGC121330.13279280797741877No Hit
CGCGACCTCAGATCAGACGTGGC119400.13068046874230446No Hit
TGTAAACATCCCCGACTGGAAGC118040.12919198099113582No Hit
CGCCTGTCTGAGCGTCGCT117410.12850246092993273No Hit
TTCACAGTGGCTAAGTTCTGC116930.1279771123118732No Hit
ACCACAGGGTAGAACCACGGAC116880.12792238849749202No Hit
CCCATAAAGTAGAAAGCACTA116150.12712342080752648No Hit
TGAGAACTGAATTCCATAGGCTGT115400.1263025635918085No Hit
TTCACAGTGGCTAAGTTCTG113970.12473746250050619No Hit
TATTGCACTTGTCCCGGCCTGTTT113650.12438723008846651No Hit
ACTGGACTTGGAGTCAGAAGGCA108710.118980517227604No Hit
ATGCACCTGGGCAAGGATTCTGA108220.11844422384666825No Hit
CTCCCACTGCTTCACTTGACTAGCCT108110.1183238314550296No Hit
TTCAAGTAATCCAGGATAGGC106170.11620054745703906No Hit
AGCAGCATTGTACAGGGCTATG102220.11187736612092432No Hit
CGACGGAATCCCAAAAGCAGCTG101400.11097989556507262No Hit
CTACGGAATCCCAAAAGCAGCTG100840.11036698884400321No Hit
CCCCCCACTGCTAAATTTGACTGGCT100580.11008242500922095No Hit
CAAGGGAATCCCAAAAGCAGCTG99470.10886755632995833No Hit
ACTGGACTTGGAGTCAGAAGG92010.10070276322428336No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)