FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5459036
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG3041725.571899507532099No Hit
AAGCTGCCAGTTGAAGAACTGT2239724.102775654895846No Hit
AACATTCAACGCTGTCGGTGAGT2222604.071414806570244No Hit
TTCAAGTAATCCAGGATAGGCT1774253.2501159545384932No Hit
TAGCAGCACGTAAATATTGGCG1594082.9200759987660825No Hit
TAGCTTATCAGACTGATGTTGAC1286772.357137780370014No Hit
AACATTCAACGCTGTCGGTGAG1053011.9289303093073578No Hit
TAGCTTATCAGACTGATGTTGACT1043381.9112898321242064No Hit
CCCCCCACTGCTAAATTTGACTG865721.5858477577359813No Hit
TAGCTTATCAGACTGATGTTGA829971.5203600049532555No Hit
CATAAAGTAGAAAGCACTACT640171.1726795719976935No Hit
TATTGCACTTGTCCCGGCCTGTAA606991.1118996101143133No Hit
TGTAAACATCCTTGACTGGAAGCT582411.0668733454038406No Hit
CGCCTGTCTGAGCGTCGCT559031.0240452709965642No Hit
TATTGCACTTGTCCCGGCCTGT551691.010599673642013No Hit
TATTGCACTTGTCCCGGCCTGTAT537780.9851189843774616No Hit
TGAGGTAGTAGGTTGTATAGTT441490.808732530798478No Hit
AGCAGCATTGTACAGGGCTATGA405880.7435012335511251No Hit
CCCCCACTGCTAAATTTGACTG342300.6270337839867698No Hit
CCCATAAAGTAGAAAGCACTAC335210.6140461429453845No Hit
TGTAAACATCCCCGACTGGAAGCT300880.5511595820214411No Hit
TTCAAGTAATTCAGGATAGGTT298940.5476058410312736No Hit
ACTGGACTTGGAGTCAGAAGGC297420.5448214666472249No Hit
TATTGCACTTGTCCCGGCCTGTA291860.5346365182424149No Hit
TGAGGTAGTAGATTGTATAGTT287020.5257704840195229No Hit
TCGTACGACTCTTAGCGG248140.4545491181959599No Hit
CCCATAAAGTAGAAAGCACT212960.39010550580725245No Hit
CAACGGAATCCCAAAAGCAGCTGT204740.37504790223035717No Hit
CATTGCACTTGTCTCGGTCTGA198290.3632326293506766No Hit
AACATTCAACGCTGTCGGTGAGTT193160.35383536580451197No Hit
TGTAAACATCCTACACTCTCAGCT185280.33940058281352237No Hit
TGATGTAGTAGGTTGTATAGTT165870.3038448546593208No Hit
TGTCAGTTTGTCAAATACCCCAA151490.2775032075260174No Hit
CAACACCAGTCGATGGGCTGT151460.2774482527684375No Hit
TTATAAAGCAATGAGACTGATT149400.27367469274795037No Hit
TTAATGCTAATCGTGATAGGGGTT149260.27341823721257746No Hit
CTACGCCTGTCTGAGCGTCGCT142290.2606504152015118No Hit
TAGCTTATCAGACTGATGTTGACA142210.2605038691812987No Hit
TACGCCTGTCTGAGCGTCGCT136220.24953123591784335No Hit
TGAGGTAGTAGTTTGTACAGTT132600.24290002850320094No Hit
CGCGACCTCAGATCAGACGTGGCG131540.24095829373537744No Hit
ACGCCTGTCTGAGCGTCGCT130390.23885169469481426No Hit
TTCAAGTAATCCAGGATAGGCTT124180.22747605987577293No Hit
ACTTGACTTGGAGTCAGAAGGC120540.22080821595607722No Hit
TTCTCACTACTGCACTTGACTAG113620.2081319852076447No Hit
TGATGTAGTAGATTGTATAGTT108220.19824012884326098No Hit
TGTCAGTTTGTCAAATACCCCA104370.19118760162050588No Hit
CCCCCACTGCTAAATTTGACTGGCTT103090.1888428652970964No Hit
CAAAGAATTCTCCTTTTGGGCT98250.1799768310742043No Hit
CAAAGAATTCTCCTTTTGGGCTT95090.17418826327578715No Hit
CACCCGGCTGTGTGCACATGTGC94020.17222821025543705No Hit
AGCAGCATTGTACAGGGCTATGT93340.17098256908362575No Hit
TGCAAGTAATCCAGGATAGGCT91740.1680516486793639No Hit
TTCACAGTGGCTAAGTTCCG90910.16653123371965306No Hit
GCGTTCGCGCTTTCCCCTG87590.16044957388080971No Hit
TTCACAGTGGCTAAGTTCCGC87470.1602297548504901No Hit
TAGCAGCACGTAAATATTGGC87400.16010152708280365No Hit
ACCACAGGGTAGAACCACGGAC87030.15942375173931808No Hit
TGTAAACATCCTACACTCAGCT86170.15784838202202733No Hit
CTTCTCACTACTGCACTTGACTAG85810.15718892493106842No Hit
TTCAAGTAATCCAGGATAGGC84910.15554028220367114No Hit
TGAGGTAGTAGTTTGTGCTGTT83940.15376341170858737No Hit
TATTGCACTTGTCCCGGCCTGTT83940.15376341170858737No Hit
TAGCAGCACGTAAATATTGGCGT82190.15055771751642597No Hit
CGCGACCTCAGATCAGACGTGG81500.14929375809208806No Hit
CACTAGATTGTGAGCTCCTGGA79310.14528206078875464No Hit
AACATTCAACGCTGTCGGTGA76990.14103222620257494No Hit
TGTAAACATCCCCGACTGGAAGC76060.13932862871759774No Hit
CAACGGAATCCCAAAAGCAGCT73860.13529861316173772No Hit
TTTAAACATCCTTGACTGGAAGCT73770.13513374888899798No Hit
AGCAGCATTGTACAGGGCTATG73120.13394306247476662No Hit
ATCCCGGACGAGCCCCCA72380.13258751178779551No Hit
ACTGGACTTGGAGTCAGAAGGCA71000.13005959293911964No Hit
TTCACAGTGGCTAAGTTCTGC70160.12852085972688218No Hit
CCCCCCACTGCTAAATTTGACTGGCT67400.1234650220295305No Hit
ACTGGACTTGGAGTCAGAAGG67080.12287883794867811No Hit
CTCCCACTGCTTCACTTGACTAG64700.11851909384733861No Hit
ACTGGACTTGGAGTCAGAAGGCT64030.11729177092805396No Hit
TATTGCACTTGTCCCGGCCTGTAG63500.11632090354414222No Hit
GCCTGTCTGAGCGTCGCT63210.11578967422086975No Hit
ACCGGGTGCTGTAGGCTT62480.1144524417864253No Hit
CACTAGATTGTGAGCTCCTGGAA62180.1139028942106262No Hit
TTCACAGTGGCTAAGTTCTG60360.11056897225077834No Hit
TCGTTCGCGCTTTCCCCTG60350.1105506539982517No Hit
AACATTCAACGCTGTCGGTGAGA58670.10747318757377677No Hit
CAAAGTGCTGTTCGTGCAGGTAG57490.10531163377563366No Hit
CAAAGTGCTGTTCGTGCAGGT56800.10404767435129572No Hit
AACGGAATCCCAAAAGCAGCTG56480.10346149027044335No Hit
AGCTCGGTCTGAGGCCCCTCAGT55540.10173957453293951No Hit
CCCATAAAGTAGAAAGCACTA55480.10162966501777969No Hit
CCTCGACACAAGGGTTTGT55070.10087861666418758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)