FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6014071
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4832508.035322496192679No Hit
AAGCTGCCAGTTGAAGAACTGT2610274.340271340328374No Hit
TAGCAGCACGTAAATATTGGCG2187253.636887559192434No Hit
TTCAAGTAATCCAGGATAGGCT1877293.1214962377397937No Hit
AACATTCAACGCTGTCGGTGAGT1713102.8484864910972947No Hit
TAGCTTATCAGACTGATGTTGAC1641772.72988130668893No Hit
CCCCCCACTGCTAAATTTGACTG1191111.9805386401324492No Hit
TAGCTTATCAGACTGATGTTGACT1017321.6915663283655946No Hit
TGTAAACATCCTTGACTGGAAGCT899881.4962909483443079No Hit
AACATTCAACGCTGTCGGTGAG872581.450897403771921No Hit
TAGCTTATCAGACTGATGTTGA768581.2779696149247324No Hit
TATTGCACTTGTCCCGGCCTGTAA633131.0527477976232738No Hit
TATTGCACTTGTCCCGGCCTGTAT558790.9291376839415432No Hit
AGCAGCATTGTACAGGGCTATGA546470.908652392031953No Hit
CATAAAGTAGAAAGCACTACT505250.84011312802925No Hit
TATTGCACTTGTCCCGGCCTGT492220.8184472714073379No Hit
TGTAAACATCCCCGACTGGAAGCT451400.7505731142848163No Hit
TTAATGCTAATCGTGATAGGGGTT446570.7425419487066248No Hit
TTCAAGTAATTCAGGATAGGTT429210.713676310106748No Hit
CCCATAAAGTAGAAAGCACTAC407600.6777439109049427No Hit
TGAGGTAGTAGGTTGTATAGTT406900.6765799738646251No Hit
CAACGGAATCCCAAAAGCAGCTGT398150.6620307608606549No Hit
CCCCCACTGCTAAATTTGACTG365680.6080407098619222No Hit
TGTAAACATCCTACACTCTCAGCT333460.5544663506633029No Hit
TGAGGTAGTAGATTGTATAGTT319810.5317695783771093No Hit
CCCCCACTGCTAAATTTGACTGGCTT272080.45240569989945245No Hit
TATTGCACTTGTCCCGGCCTGTA259330.43120541809366736No Hit
ACTGGACTTGGAGTCAGAAGGC254640.42340703992353934No Hit
TAGCTTATCAGACTGATGTTGACA247780.41200045692842663No Hit
CAAAGAATTCTCCTTTTGGGCT243890.4055322925186617No Hit
TGTCAGTTTGTCAAATACCCCAA229740.3820041366322413No Hit
TGTCAGTTTGTCAAATACCCCA198960.33082416220227534No Hit
TTCTCACTACTGCACTTGACTAG193750.3221611450879113No Hit
CAAAGAATTCTCCTTTTGGGCTT175430.2916992499755989No Hit
CATTGCACTTGTCTCGGTCTGA169550.28192217883693094No Hit
CGCCTGTCTGAGCGTCGCT167140.27791490988383744No Hit
CAACGGAATCCCAAAAGCAGCT166660.2771167816276196No Hit
AACATTCAACGCTGTCGGTGAGTT163750.27227812907429927No Hit
CTTCTCACTACTGCACTTGACTAG147470.24520827905091241No Hit
CCCCCCACTGCTAAATTTGACTGGCT147460.24519165137890792No Hit
CTACGCCTGTCTGAGCGTCGCT143610.23878999765716102No Hit
GCCTGTCTGAGCGTCGCT143610.23878999765716102No Hit
TAGCAGCACGTAAATATTGGC142300.23661177262456662No Hit
TGAGGTAGTAGTTTGTACAGTT134960.2244070613732362No Hit
TGATGTAGTAGGTTGTATAGTT133740.22237848538868266No Hit
TGTAAACATCCCCGACTGGAAGC132230.2198677069159975No Hit
TGTAAACATCCTACACTCAGCT130250.2165754278590991No Hit
CCCATAAAGTAGAAAGCACT130040.21622624674700383No Hit
CACCCGTAGAACCGACCTTGCG120080.1996650854304846No Hit
TAGCAGCACGTAAATATTGGCGT118070.1963229233575726No Hit
TTCACAGTGGCTAAGTTCCGC115640.19228239906047No Hit
TTCAAGTAATCCAGGATAGGCTT110620.18393530771419225No Hit
ATCACATTGCCAGGGATTTCCA109490.18205638077767955No Hit
TGATGTAGTAGATTGTATAGTT104970.17454067303162865No Hit
AGCTCGGTCTGAGGCCCCTCAGT99490.1654287087731422No Hit
TTCACAGTGGCTAAGTTCCG99060.16471371887694708No Hit
CGCGACCTCAGATCAGACGTGGCG99000.16461395284491984No Hit
TGAGGTAGTAGTTTGTGCTGTT97710.16246898315633454No Hit
CACCCGGCTGTGTGCACATGTGC96370.16024087510772653No Hit
ATCACATTGCCAGGGATTTCC96240.16002471537166754No Hit
AGCAGCATTGTACAGGGCTATGT94720.15749730922697788No Hit
TCGTACGACTCTTAGCGG94130.15651627657871017No Hit
ACTTGACTTGGAGTCAGAAGGC89830.1493663776167591No Hit
TTAATGCTAATCGTGATAGGGGTTT85500.1421665956387944No Hit
TTATAAAGCAATGAGACTGATT84590.14065347748638152No Hit
TTCAAGTAATCCAGGATAGGC84480.1404705730943316No Hit
TTCACAGTGGCTAAGTTCTGC83950.13958930647809115No Hit
TATTGCACTTGTCCCGGCCTGTT82860.13777689022959655No Hit
CTCCCACTGCTTCACTTGACTAGCCT82570.13729468774146497No Hit
TACGCCTGTCTGAGCGTCGCT81500.13551552683697948No Hit
CTCCCACTGCTTCACTTGACTAG81480.1354822714929704No Hit
TGTCAGTTTGTCAAATACCCC81420.13538250546094316No Hit
TCGTTTCCCGGCCAATGCACCA78770.1309761723797408No Hit
ACTGGACTTGGAGTCAGAAGGCA78200.13002839507548214No Hit
GGAATACCGGGTGCTGTAGGCTTT75340.12527288088218447No Hit
GGGCTACGCCTGTCTGAGCGTCGCT75320.1252396255381754No Hit
CAACACCAGTCGATGGGCTGT75190.1250234658021164No Hit
TAGCAGCACATCATGGTTTACA73240.12178106976123161No Hit
CCCCCCACTGCTAAATTTGACTGG72810.12106607986503651No Hit
CAAAGTGCTGTTCGTGCAGGTAG72170.12000190885674612No Hit
AGCAGCATTGTACAGGGCTATG71090.11820612028025608No Hit
CACTAGATTGTGAGCTCCTGGA70060.11649347006378874No Hit
TCCTGTCTGAGCGTCGCT68880.11453140476725333No Hit
TATTGCACTTGTCCCGGCCTGTAG68330.11361688280700377No Hit
TGAGAACTGAATTCCATGGGTT68100.11323444635089942No Hit
CACTAGATTGTGAGCTCCTGGAA68030.11311805264686764No Hit
AACCGGGCGGAAACACCA67120.11160493449445476No Hit
AACGGAATCCCAAAAGCAGCTG65860.10950984782188306No Hit
TGTAAACATCCTTGACTGGAAGC64410.10709883538122514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)