FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14968477
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG12305148.220702747513991No Hit
AACATTCAACGCTGTCGGTGAGT7078594.728998147239696No Hit
TTCAAGTAATCCAGGATAGGCT6286444.19978599025138No Hit
AAGCTGCCAGTTGAAGAACTGT6256434.179737190363455No Hit
TAGCAGCACGTAAATATTGGCG5498433.673339645710115No Hit
CCCCCCACTGCTAAATTTGACTG5057123.378513391843405No Hit
AACATTCAACGCTGTCGGTGAG4460202.9797286657820967No Hit
TAGCTTATCAGACTGATGTTGACT2402951.605340342908634No Hit
TGAGGTAGTAGGTTGTATAGTT2307361.5414794704898835No Hit
TAGCTTATCAGACTGATGTTGAC2199701.469554985453764No Hit
TAGCTTATCAGACTGATGTTGA2117671.4147531509050655No Hit
CCCCCACTGCTAAATTTGACTG2060441.3765194682130988No Hit
CATAAAGTAGAAAGCACTACT1677231.1205081184946204No Hit
TATTGCACTTGTCCCGGCCTGTAA1619991.08226775509626No Hit
CGCCTGTCTGAGCGTCGCT1514451.0117595798156351No Hit
TATTGCACTTGTCCCGGCCTGT1436460.9596567506500495No Hit
TATTGCACTTGTCCCGGCCTGTAT1321020.8825346760395196No Hit
TTCAAGTAATTCAGGATAGGTT1213030.8103897276924031No Hit
TGTAAACATCCTTGACTGGAAGCT1169280.7811616372193377No Hit
TCGTACGACTCTTAGCGG1116250.7457338512127853No Hit
TGAGGTAGTAGATTGTATAGTT1077900.7201133421923954No Hit
AGCAGCATTGTACAGGGCTATGA946510.632335540883685No Hit
ACTGGACTTGGAGTCAGAAGGC805610.5382043877944296No Hit
TATTGCACTTGTCCCGGCCTGTA741500.4953743791034987No Hit
CAACGGAATCCCAAAAGCAGCTGT729380.48727736295416024No Hit
CCCATAAAGTAGAAAGCACTAC701360.46855802363861065No Hit
TGTAAACATCCCCGACTGGAAGCT648350.433143599044846No Hit
CCCATAAAGTAGAAAGCACT627210.41902058572826073No Hit
ACCGGGTGCTGTAGGCTT625790.41807192542033506No Hit
CAACACCAGTCGATGGGCTGT618620.41328185893594915No Hit
TTCTCACTACTGCACTTGACTAG576350.3850425130091725No Hit
CATTGCACTTGTCTCGGTCTGA563290.37631751045881284No Hit
CCCCCACTGCTAAATTTGACTGGCTT516200.34485806405020364No Hit
AACATTCAACGCTGTCGGTGAGTT480230.3208275631515484No Hit
CGCGACCTCAGATCAGACGTGGCG450690.3010927564641346No Hit
TACGCCTGTCTGAGCGTCGCT448600.2996964888278213No Hit
TGAGGTAGTAGTTTGTACAGTT446320.29817328777002494No Hit
CTACGCCTGTCTGAGCGTCGCT429420.2868828939644294No Hit
CACCCGGCTGTGTGCACATGTGC425110.2840035095086828No Hit
CTCCCACTGCTTCACTTGACTAG386940.258503253203382No Hit
TTCACAGTGGCTAAGTTCCG358800.23970374541110628No Hit
ACCGGGTGCTGTAGGCTTA356900.23843441119627604No Hit
TGTAAACATCCTACACTCTCAGCT356880.23842104978348835No Hit
AGCTCGGTCTGAGGCCCCTCAGT353230.23598259194973545No Hit
TGAGGTAGTAGTTTGTGCTGTT345100.23055117765154062No Hit
CTTCTCACTACTGCACTTGACTAG341160.2279189793323663No Hit
TTCAAGTAATCCAGGATAGGC341150.22791229862597243No Hit
AACGGAATCCCAAAAGCAGCTG337480.22546047937943184No Hit
CGCGACCTCAGATCAGACGTGG321690.21491164398355292No Hit
ACGCCTGTCTGAGCGTCGCT320070.21382936954775023No Hit
ACCGGGTGCTGTAGGCTTT304760.2036012080587758No Hit
CCCCCCACTGCTAAATTTGACTGGCT296800.19828336576927633No Hit
TTCAAGTAATCCAGGATAGGCTT295740.1975752108915289No Hit
TGAGGTAGTAGGTTGTGTGGTT294730.1969004595457507No Hit
TAGCAGCACGTAAATATTGGC288310.19261144604090316No Hit
TGAGGTAGTAGGTTGTATAGT282660.18883684692838157No Hit
TTCACAGTGGCTAAGTTCTG270430.1806663430087109No Hit
TAGCTTATCAGACTGATGTTGACA261640.17479400208852242No Hit
CAAAGTGCTGTTCGTGCAGGT257430.1719814246967143No Hit
AGAGGTAGTAGGTTGCATAGTT250530.16737173728496227No Hit
AACATTCAACGCTGTCGGTGA236570.15804547115915668No Hit
TATTGCACTTGTCCCGGCCTGTT228310.15252720767784192No Hit
CAAAGAATTCTCCTTTTGGGCT223480.14930042648961547No Hit
TTATAAAGCAATGAGACTGATT223360.14922025801288935No Hit
TTCACAGTGGCTAAGTTCCGC221030.14766365342312382No Hit
ACTGGACTTGGAGTCAGAAGG220850.14754340070803462No Hit
CCCCCCACTGCTAAATTTGACTGG219610.14671499311519803No Hit
GCCTGTCTGAGCGTCGCT216260.14447695647326045No Hit
AGCAGCATTGTACAGGGCTATGT216070.1443500230517774No Hit
CTCCCACTGCTTCACTTGACTAGCCT208630.13937957749475782No Hit
TTCACAGTGGCTAAGTTCTGC205870.137535702530057No Hit
CAACGGAATCCCAAAAGCAGCT203030.13563838191420544No Hit
AATGACACGATCACTCCCGTTG202320.13516405176024254No Hit
AGCAGCATTGTACAGGGCTATG199130.13303290642060647No Hit
ACCACAGGGTAGAACCACGGAC195290.13046751516537053No Hit
CACTAGATTGTGAGCTCCTGGA194480.1299263779474692No Hit
AACCGGGCGGAAACACCA185280.12378012806513315No Hit
CGCGACCTCAGATCAGACGTGGC182940.12221684276897377No Hit
GGAATACCGGGTGCTGTAGGCTTT179510.11992536047588542No Hit
GCGTTCGCGCTTTCCCCTG178960.11955792162422403No Hit
ACTGGACTTGGAGTCAGAAGGCT175520.11725975862474185No Hit
GGAATACCGGGTGCTGTAGGCTT174090.11630441761042222No Hit
CGCGACCTCAGATCAGACGT173670.1160238279418808No Hit
AACATTCAACGCTGTCGGTGAGA172610.11531567306413337No Hit
TATTGCACTTGTCCCGGCCTGTAG167080.11162124242833789No Hit
CCCATAAAGTAGAAAGCACTA165160.11033854680071993No Hit
ATCCCGGACGAGCCCCCA163750.109396567199188No Hit
CAAAGAATTCTCCTTTTGGGCTT162010.10823412428665923No Hit
TGTAAACATCCCCGACTGGAAGC156710.10469334989792216No Hit
CGCGACCTCAGATCAGACGTG155850.1041188091480516No Hit
CAAAGTGCTGTTCGTGCAGGTAG155590.10394511078181166No Hit
CACTAGATTGTGAGCTCCTGGAA153880.10280270998846443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)