FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6365762
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5631988.847299035056604No Hit
CCCCCCACTGCTAAATTTGACTG5445178.55383848783539No Hit
AACATTCAACGCTGTCGGTGAGT2335073.6681704405536997No Hit
TTCAAGTAATCCAGGATAGGCT1845102.8984746837849107No Hit
AACATTCAACGCTGTCGGTGAG1748842.747259479697796No Hit
CCCCCACTGCTAAATTTGACTG1583592.487667619367485No Hit
TAGCAGCACGTAAATATTGGCG1299412.0412481647915834No Hit
TAGCTTATCAGACTGATGTTGACT1277192.0063426813632055No Hit
TAGCTTATCAGACTGATGTTGAC1265411.987837434073093No Hit
TATTGCACTTGTCCCGGCCTGTAA1214231.907438575303318No Hit
AAGCTGCCAGTTGAAGAACTGT1204001.8913682289724307No Hit
TATTGCACTTGTCCCGGCCTGTAT1137601.787060213686908No Hit
TGAGGTAGTAGGTTGTATAGTT937881.4733192978311158No Hit
TAGCTTATCAGACTGATGTTGA844191.3261413166247813No Hit
GCCTGTCTGAGCGTCGCT625780.9830402079122657No Hit
TATTGCACTTGTCCCGGCCTGT565370.8881419066562652No Hit
TGAGGTAGTAGATTGTATAGTT544630.855561360917986No Hit
TATTGCACTTGTCCCGGCCTGTA498000.7823101146414208No Hit
TTCAAGTAATTCAGGATAGGTT442760.6955333862623202No Hit
TCCTGTCTGAGCGTCGCT413960.6502913555360694No Hit
AGCAGCATTGTACAGGGCTATGA408880.6423111640051891No Hit
TGTAAACATCCTTGACTGGAAGCT388840.6108302509581728No Hit
TCGTACGACTCTTAGCGG334530.5255144631546074No Hit
TGATGTAGTAGGTTGTATAGTT330590.5193251020066412No Hit
TGTAAACATCCCCGACTGGAAGCT304700.47865440146835525No Hit
CACCCGGCTGTGTGCACATGTGC298580.4690404699390269No Hit
CAACGGAATCCCAAAAGCAGCTGT288810.4536927393766842No Hit
CAACGGAATCCCAAAAGCAGCT284470.44687501669085333No Hit
ACTGGACTTGGAGTCAGAAGGC273130.42906096709239205No Hit
CATTGCACTTGTCTCGGTCTGA269960.4240812019048152No Hit
GGAATACCGGGTGCTGTAGGCTTT242720.38128978117623624No Hit
CCCCCACTGCTAAATTTGACTGGCTT236380.37133025080108245No Hit
CATAAAGTAGAAAGCACTACT223380.3509085008204831No Hit
TAGCTTATCAGACTGATGTTGACA215190.3380427983327055No Hit
TGATGTAGTAGATTGTATAGTT193890.3045825464414158No Hit
CGCGACCTCAGATCAGACGTGG164610.2585864818697275No Hit
CCCATAAAGTAGAAAGCACTAC162590.2554132561035113No Hit
TGAATACCGGGTGCTGTAGGCTTT157260.24704033861146554No Hit
TGAGGTAGTAGTTTGTACAGTT156420.24572077938194986No Hit
GGAATACCGGGTGCTGTAGGCTT152230.23913869227281825No Hit
CACTAGATTGTGAGCTCCTGGAA150640.2366409551598065No Hit
CACTAGATTGTGAGCTCCTGGA150390.2362482291986411No Hit
AACATTCAACGCTGTCGGTGAGTT146140.22957188785882982No Hit
TATTGCACTTGTCCCGGCCTGTAG145080.2279067297834886No Hit
TATTGCACTTGTCCCGGCCTGTAAT135010.2120877280677474No Hit
CAAAGAATTCTCCTTTTGGGCT133040.2089930474937643No Hit
TCGTTTCCCGGCCAATGCACCA131650.20680949114968483No Hit
TTCAAGTAATCCAGGATAGGCTT130490.2049872426898775No Hit
CCCCCCACTGCTAAATTTGACTGGCT128660.202112488654147No Hit
CCCATAAAGTAGAAAGCACT128020.20110711019356362No Hit
ACCCCCCACTGCTAAATTTGACTG123940.19469782250734474No Hit
CTCCCACTGCTTCACTTGACTAG123250.19361389885452834No Hit
CTCCCACTGCTTCACTTGACTAGCCT119860.18828853482112587No Hit
TGTAAACATCCTACACTCTCAGCT118040.185429489823842No Hit
TAGCAGCACGTAAATATTGGC115410.18129801271238227No Hit
TATTGCACTTGTCCCGGCCTGTT112750.17711940848558272No Hit
CGCGACCTCAGATCAGACGTGGCG111090.17451170810344466No Hit
AACCGGGCGGAAACACCA109020.17125993714499538No Hit
ACTTGACTTGGAGTCAGAAGGC107210.16841660118615806No Hit
CCCCCCACTGCTAAATTTGACTGG105080.1650705759970291No Hit
TATTGCACTTGTCCCGGCCTGTAAA102710.1613475338851814No Hit
CAACACCAGTCGATGGGCTGT102540.1610804802315889No Hit
CCCGCCACTGCTAAATTTGACTG100730.15823714427275162No Hit
TGAATACCGGGTGCTGTAGGCTT99840.1568390398510029No Hit
TGAGGTAGTAGTTTGTGCTGTT98240.15432559369954454No Hit
AACGGAATCCCAAAAGCAGCTG97070.15248763620129058No Hit
ACCGGGTGCTGTAGGCTT96470.1515450938944937No Hit
AGCAGCATTGTACAGGGCTATGT90930.14284228659506906No Hit
TGCAAGTAATCCAGGATAGGCT88780.13946484332904688No Hit
CAAAGAATTCTCCTTTTGGGCTT86620.13607169102457806No Hit
TTATAAAGCAATGAGACTGATT86620.13607169102457806No Hit
CTCCCCACTGCTAAATTTGACTG85030.1335739539115663No Hit
ATGCACCTGGGCAAGGATTCTGA82310.12930109545408705No Hit
AGCTCGGTCTGAGGCCCCTCAGT79560.12498110988126794No Hit
TATTGCACTTGTCCCGGCCTGTTT78900.12394431334379137No Hit
TTCAAGTAATCCAGGATAGGC78470.12326882469058692No Hit
CGCCCCACTGCTAAATTTGACTG77320.12146228526922621No Hit
CAACGGAATCCCAAAAGCAGCTGA74810.11751931661912589No Hit
ACCGGGTGCTGTAGGCTTT74680.1173150991193199No Hit
CTACGGAATCCCAAAAGCAGCTG72880.11448747219892921No Hit
CGCGACCTCAGATCAGACGTGGC72700.11420470950689016No Hit
TGAGAACTGAATTCCATAGGCTGT71400.11216253450883021No Hit
TGAGGTAGTAGGTTGTATAGT71190.11183264470145128No Hit
TTCTCACTACTGCACTTGACTAG70010.10997897816475075No Hit
TGTAAACATCCCCGACTGGAAGC69540.10924065335775983No Hit
CAAAGTGCTGTTCGTGCAGGTAG68250.10721418739814653No Hit
TTAGGTAGTAGGTTGTATAGTT67030.1052976847076595No Hit
ACCGGGTGCTGTAGGCTTA66990.10523484855387305No Hit
CGACGGAATCCCAAAAGCAGCTG66730.10482641355426107No Hit
AATGCACCCGGGCAAGGATTCT65670.10316125547891988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)