FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6350347
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG4358716.863735162818661No Hit
CAACGGAATCCCAAAAGCAGCTG3770425.937344841155924No Hit
AACATTCAACGCTGTCGGTGAGT2892634.555073919582662No Hit
TAGCAGCACGTAAATATTGGCG2224663.503210139540406No Hit
TTCAAGTAATCCAGGATAGGCT1934013.045518614967025No Hit
AAGCTGCCAGTTGAAGAACTGT1892822.980656017694781No Hit
TAGCTTATCAGACTGATGTTGAC1326982.0896180948852088No Hit
AACATTCAACGCTGTCGGTGAG1195441.8824798077963298No Hit
TAGCTTATCAGACTGATGTTGACT1161551.829112645340483No Hit
CCCCCACTGCTAAATTTGACTG992551.56298545575541No Hit
TATTGCACTTGTCCCGGCCTGTAT905891.4265204720308984No Hit
TATTGCACTTGTCCCGGCCTGTAA895271.4097969764486886No Hit
TAGCTTATCAGACTGATGTTGA792391.2477900813924028No Hit
TATTGCACTTGTCCCGGCCTGT552050.8693225740262698No Hit
TGTAAACATCCTTGACTGGAAGCT507740.7995468594078403No Hit
TGAGGTAGTAGGTTGTATAGTT404480.6369415718542625No Hit
TATTGCACTTGTCCCGGCCTGTA395270.6224384273804251No Hit
AGCAGCATTGTACAGGGCTATGA377910.5951013385567749No Hit
TTAATGCTAATCGTGATAGGGGTT351880.5541114524922811No Hit
TGTAAACATCCCCGACTGGAAGCT333060.5244752767053518No Hit
ACTGGACTTGGAGTCAGAAGGC331190.5215305557318364No Hit
TTCAAGTAATTCAGGATAGGTT307010.4834538962988952No Hit
CCCCCACTGCTAAATTTGACTGGCTT293980.46293533251017627No Hit
CATAAAGTAGAAAGCACTACT291590.45917175864562987No Hit
CCCATAAAGTAGAAAGCACTAC273520.4307166206823029No Hit
CAACGGAATCCCAAAAGCAGCTGT270670.4262286769526138No Hit
AACATTCAACGCTGTCGGTGAGTT252310.39731687103082713No Hit
TGAGGTAGTAGATTGTATAGTT225920.3557600868110042No Hit
CCCCCCACTGCTAAATTTGACTGGCT224030.35278387149552615No Hit
TGTAAACATCCTACACTCTCAGCT204010.3212580351908329No Hit
CAAAGAATTCTCCTTTTGGGCT184170.29001564796380414No Hit
TAGCTTATCAGACTGATGTTGACA181560.28590563633766786No Hit
CATTGCACTTGTCTCGGTCTGA168230.2649146574195079No Hit
TTCAAGTAATCCAGGATAGGCTT164690.25934015889210466No Hit
TTTAAACATCCTTGACTGGAAGCT154360.24307333126835431No Hit
CAAAGAATTCTCCTTTTGGGCTT154240.2428843652165779No Hit
ACTTGACTTGGAGTCAGAAGGC152520.24017585180778311No Hit
TCGTACGACTCTTAGCGG147060.23157789645195767No Hit
CCCCCCACTGCTAAATTTGACTGG144970.22828673771685232No Hit
CACTAGATTGTGAGCTCCTGGA144100.22691673384147354No Hit
GGAATACCGGGTGCTGTAGGCTTT143630.22617661680534937No Hit
CGCGACCTCAGATCAGACGTGGCG137110.2159094613254992No Hit
TGATGTAGTAGGTTGTATAGTT131240.20666587195943778No Hit
CACTAGATTGTGAGCTCCTGGAA128000.20156378856147544No Hit
CCCATAAAGTAGAAAGCACT123800.19494997674930203No Hit
CGCCTGTCTGAGCGTCGCT120770.19017858394194836No Hit
CTCCCACTGCTTCACTTGACTAG120160.1892180065120851No Hit
CAACGGAATCCCAAAAGCAGCT116530.1835017834458495No Hit
CACCCGGCTGTGTGCACATGTGC114880.1809035002339242No Hit
CTTCTCACTACTGCACTTGACTAG114110.1796909680683591No Hit
TATTGCACTTGTCCCGGCCTGTAG112830.17767533018274434No Hit
CGCCCCACTGCTAAATTTGACTG110680.1742896884217508No Hit
CTACGCCTGTCTGAGCGTCGCT110290.17367554875347754No Hit
TATTGCACTTGTCCCGGCCTGTT110010.17323462796599934No Hit
CTCCCACTGCTTCACTTGACTAGCCT108530.1709040466607573No Hit
ATCAGCATTGTACAGGGCTATGA105910.16677828786363957No Hit
TGTAAACATCCCCGACTGGAAGC104500.16455793675526706No Hit
TTTAAACATCCCCGACTGGAAGCT100800.15873148349216193No Hit
CGCGACCTCAGATCAGACGTGG99520.15671584560654717No Hit
GGAATACCGGGTGCTGTAGGCTT98990.1558812455445348No Hit
TAGCAGCACGTAAATATTGGC95240.14997605642652284No Hit
TTCTCACTACTGCACTTGACTAG94670.149078467680585No Hit
TGTCAGTTTGTCAAATACCCCAA92620.14585029762940516No Hit
ACTGGACTTGGAGTCAGAAGGCA91760.1444960409250077No Hit
TAGCAGCACGTAAATATTGGCGT91570.14419684467636176No Hit
TGAGGTAGTAGTTTGTACAGTT87500.1377877460869461No Hit
AACATTCAACGCTGTCGGTGAGA87370.13758303286418835No Hit
GTCAGGATGGCCGAGCGGTCTAAGG86620.13640199504058598No Hit
TGAATACCGGGTGCTGTAGGCTTT86200.13574061385936861No Hit
TATTGCACTTGTCCCGGCCTGTAAT85460.1345753232067476No Hit
TTCAAGTAATCCAGGATAGGC84230.13263842117603966No Hit
ACCCCCCACTGCTAAATTTGACTG84130.13248094946622602No Hit
AACATTCAACGCTGTCGGTGA83290.13115818710379132No Hit
TGCAAGTAATCCAGGATAGGCT82200.12944174546682252No Hit
CCCCCACTGCTAAATTTGACTGGCT82110.12930002092799023No Hit
TATTGCACTTGTCCCGGCCTGTTT80560.1268592094258786No Hit
AGCTCGGTCTGAGGCCCCTCAGT80390.1265915075191954No Hit
CCCGCCACTGCTAAATTTGACTG78860.12418219035904651No Hit
AGCAGCATTGTACAGGGCTATGT77790.12249724306404043No Hit
ATCACATTGCCAGGGATTTCCA75450.11881240505440095No Hit
CGCGACCTCAGATCAGACGTGGC73310.11544251046438879No Hit
ACCGGGTGCTGTAGGCTT73240.11533228026751924No Hit
TGATGTAGTAGATTGTATAGTT73070.11506457836083603No Hit
TTAGGTAGTAGGTTGTATAGTT72880.11476538211219009No Hit
TTATGTAGTAGGTTGTATAGTT70650.11125376298334562No Hit
TGAGGTAGTAGTTTGTGCTGTT70160.11048215160525873No Hit
TTATAAAGCAATGAGACTGATT70140.110450657263296No Hit
TGTAAACATCCTACACTCAGCT69270.10908065338791724No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC67470.10624616261127148No Hit
TGTCAGTTTGTCAAATACCCCA67040.10556903425907277No Hit
ACCACAGGGTAGAACCACGGAC65830.10366362657032756No Hit
ACTGGACTTGGAGTCAGAAGGCT65830.10366362657032756No Hit
CAAAGTGCTGTTCGTGCAGGTAG65210.10268730196948293No Hit
TACGCCTGTCTGAGCGTCGCT64200.10109683770036504No Hit
CCCTCCACTGCTAAATTTGACTG64060.10087637730662592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)