FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7130887
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4658266.532511313108734No Hit
GCCTGTCTGAGCGTCGCT3955685.547248189460862No Hit
CCCCCCACTGCTAAATTTGACTG3687455.171095825806804No Hit
AAGCTGCCAGTTGAAGAACTGT2495903.50012558045023No Hit
TAGCAGCACGTAAATATTGGCG2124632.979475063901588No Hit
TTCAAGTAATCCAGGATAGGCT2054512.881142275848713No Hit
AACATTCAACGCTGTCGGTGAGT1993062.794967863044247No Hit
GGAATACCGGGTGCTGTAGGCTTT1559862.187469805649704No Hit
TCCTGTCTGAGCGTCGCT1412121.980286603896542No Hit
TAGCTTATCAGACTGATGTTGAC1124171.5764798965402198No Hit
GGAATACCGGGTGCTGTAGGCTT1116881.566256764410935No Hit
CCCCCACTGCTAAATTTGACTG1065761.494568627998172No Hit
AACATTCAACGCTGTCGGTGAG1010111.4165278456943715No Hit
TAGCTTATCAGACTGATGTTGACT804821.128639396473398No Hit
TGAGGTAGTAGGTTGTATAGTT725021.0167318595849297No Hit
TAGCTTATCAGACTGATGTTGA674670.946123532738634No Hit
TATTGCACTTGTCCCGGCCTGTAA673030.9438236785970666No Hit
TGTAAACATCCTTGACTGGAAGCT667600.93620891762834No Hit
TATTGCACTTGTCCCGGCCTGTAT666320.9344139095178483No Hit
TGAATACCGGGTGCTGTAGGCTTT599810.8411436052765946No Hit
TATTGCACTTGTCCCGGCCTGT542890.7613218383631657No Hit
TTAATGCTAATCGTGATAGGGGTT537060.7531461373599104No Hit
AGCAGCATTGTACAGGGCTATGA464920.651980602132666No Hit
TGAGGTAGTAGATTGTATAGTT456180.6397240623782147No Hit
TGAATACCGGGTGCTGTAGGCTT422730.5928154519907551No Hit
TTCAAGTAATTCAGGATAGGTT365150.512068134020354No Hit
TGTAAACATCCCCGACTGGAAGCT351450.4928559378377472No Hit
TATTGCACTTGTCCCGGCCTGTA348700.48899947510036273No Hit
CATAAAGTAGAAAGCACTACT334970.46974520841516637No Hit
CAACGGAATCCCAAAAGCAGCTGT332110.46573448716828636No Hit
CCCATAAAGTAGAAAGCACTAC318510.4466625259943118No Hit
CGCCTGTCTGAGCGTCGCT312080.43764541493926357No Hit
CCCCCACTGCTAAATTTGACTGGCTT310360.4352333727907902No Hit
TGTCAGTTTGTCAAATACCCCAA267740.37546521211176115No Hit
ACTGGACTTGGAGTCAGAAGGC252410.35396718528845006No Hit
TGTAAACATCCTACACTCTCAGCT237730.33338068602124815No Hit
GGAATACCGGGTGCTGTAGGCTTA235000.32955227028559003No Hit
AACCGGGCGGAAACACCA214860.3013089395470718No Hit
TGAGGTAGTAGTTTGTACAGTT206410.28945908131765374No Hit
CATTGCACTTGTCTCGGTCTGA190550.26721780894859226No Hit
TGATGTAGTAGGTTGTATAGTT189500.26574534135795447No Hit
CCCCCCACTGCTAAATTTGACTGGCT179900.25228278052926656No Hit
TGTCAGTTTGTCAAATACCCCA178370.2501371848971944No Hit
GGAATACCGGGTGCTGTAGGCT174720.24501860708212037No Hit
AACATTCAACGCTGTCGGTGAGTT165760.23245355030867826No Hit
TAGCTTATCAGACTGATGTTGACA158210.22186580715694973No Hit
CAACGGAATCCCAAAAGCAGCT156230.21908915398603288No Hit
ACCGGGTGCTGTAGGCTT150060.21043665395342823No Hit
CCCCCCACTGCTAAATTTGACTGG147870.20736550726438382No Hit
CTACGCCTGTCTGAGCGTCGCT146450.20537417014180703No Hit
CAAAGAATTCTCCTTTTGGGCT141680.19868496023005272No Hit
CGCGACCTCAGATCAGACGTGG133750.18756432404552198No Hit
TTCTCACTACTGCACTTGACTAG130540.18306278026842945No Hit
TTCAAGTAATCCAGGATAGGCTT129840.18208113520800429No Hit
TGAGGTAGTAGTTTGTGCTGTT128230.1798233515690264No Hit
CTCCCACTGCTTCACTTGACTAG125680.1762473588489062No Hit
TGTAAACATCCCCGACTGGAAGC124770.17497122027035344No Hit
TAGCAGCACGTAAATATTGGC124190.1741578572202869No Hit
CCCATAAAGTAGAAAGCACT122100.17122694553987464No Hit
TGATGTAGTAGATTGTATAGTT119820.16802958734306123No Hit
GGGCTACGCCTGTCTGAGCGTCGCT119300.167300365298174No Hit
CGCGACCTCAGATCAGACGTGGCG119020.16690770727400392No Hit
CACCCGGCTGTGTGCACATGTGC118900.16673942526364532No Hit
TAGCAGCACGTAAATATTGGCGT113900.159727674832037No Hit
AGCTCGGTCTGAGGCCCCTCAGT113720.15947525181649913No Hit
ACCGGGTGCTGTAGGCTTT106720.1496588012122475No Hit
CTCCCACTGCTTCACTTGACTAGCCT105630.1481302396181569No Hit
CAAAGAATTCTCCTTTTGGGCTT104730.1468681245404674No Hit
TACGCCTGTCTGAGCGTCGCT104580.14665777202751917No Hit
TGTAAACATCCTACACTCAGCT102980.1444140118894045No Hit
TATTGCACTTGTCCCGGCCTGTT99810.13996856211576483No Hit
TGAATACCGGGTGCTGTAGGCTTA95220.13353177521954843No Hit
CTTCTCACTACTGCACTTGACTAG95070.13332142270660016No Hit
TTCAAGTAATCCAGGATAGGC91490.12830100939756864No Hit
AGCAGCATTGTACAGGGCTATGT90880.12744557584491242No Hit
ACCGGGTGCTGTAGGCTTA90200.12649197778621368No Hit
ACGCCTGTCTGAGCGTCGCT87360.12250930354106018No Hit
CCCTGTCTGAGCGTCGCT86610.12145754097631895No Hit
ACCCCCCACTGCTAAATTTGACTG85980.1205740604219363No Hit
AACGGAATCCCAAAAGCAGCTG85260.11956436835978469No Hit
GACCCGCGGGCGCTCTCCAGTCCT82690.11596032863793802No Hit
TTAATGCTAATCGTGATAGGGGTTT82430.11559571761549439No Hit
TTCACAGTGGCTAAGTTCCG81520.11431957903694169No Hit
ATCACATTGCCAGGGATTTCCA80270.1125666414290396No Hit
TTCACAGTGGCTAAGTTCTGC76950.1079108391424517No Hit
ATCACATTGCCAGGGATTTCC76880.10781267463640919No Hit
TATTGCACTTGTCCCGGCCTGTAG76180.10683102957598403No Hit
TGAGAACTGAATTCCATAGGCTGT74380.10430679942060503No Hit
CACTAGATTGTGAGCTCCTGGA72560.10175452226349962No Hit
CAAAGTGCTGTTCGTGCAGGTAG71910.10084299470739053No Hit
TAGCAGCACATCATGGTTTACA71380.10009974916164006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)