FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12961238
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8518216.572065106743661No Hit
CAACGGAATCCCAAAAGCAGCTG6074624.686759088908019No Hit
AACATTCAACGCTGTCGGTGAGT5862334.5229707223955No Hit
AAGCTGCCAGTTGAAGAACTGT4137093.1918941693686973No Hit
TTCAAGTAATCCAGGATAGGCT3825202.9512612915525507No Hit
TAGCAGCACGTAAATATTGGCG3442822.656243176770614No Hit
AACATTCAACGCTGTCGGTGAG2648782.0436165125584456No Hit
CCCCCACTGCTAAATTTGACTG2568561.9817242766470302No Hit
TAGCTTATCAGACTGATGTTGAC2092161.6141667948694407No Hit
TAGCTTATCAGACTGATGTTGACT1823061.40654773872681No Hit
TAGCTTATCAGACTGATGTTGA1610201.2423195994086367No Hit
TATTGCACTTGTCCCGGCCTGTAA1502691.159372276012523No Hit
TATTGCACTTGTCCCGGCCTGTAT1487501.1476527165074817No Hit
TATTGCACTTGTCCCGGCCTGT1484491.1453304074811372No Hit
GCCTGTCTGAGCGTCGCT1463471.1291128208586247No Hit
TTAATGCTAATCGTGATAGGGGTT1199470.9254285740297339No Hit
TGAGGTAGTAGGTTGTATAGTT1145050.8834418440584147No Hit
CGCCTGTCTGAGCGTCGCT953290.7354930138617931No Hit
TGTAAACATCCTTGACTGGAAGCT919920.7097470164501262No Hit
TATTGCACTTGTCCCGGCCTGTA905920.698945579118291No Hit
AGCAGCATTGTACAGGGCTATGA726390.5604325759622654No Hit
CATAAAGTAGAAAGCACTACT706230.5448785062044228No Hit
TGAGGTAGTAGATTGTATAGTT677370.5226121146760827No Hit
ACCGGGTGCTGTAGGCTT596800.4604498428313715No Hit
TTCAAGTAATTCAGGATAGGTT588830.45430073886460537No Hit
TCGTACGACTCTTAGCGG562180.4337394313722192No Hit
GGAATACCGGGTGCTGTAGGCTTT556250.429164251130949No Hit
ACTGGACTTGGAGTCAGAAGGC543630.41942752690753765No Hit
TGTAAACATCCCCGACTGGAAGCT542860.4188334478542868No Hit
TCCTGTCTGAGCGTCGCT531350.4099531233050423No Hit
AACATTCAACGCTGTCGGTGAGTT475290.36670108210342256No Hit
ACCGGGTGCTGTAGGCTTT412340.3181331906720639No Hit
TGATGTAGTAGGTTGTATAGTT404200.31185292639483975No Hit
CAACGGAATCCCAAAAGCAGCTGT391270.30187702748765205No Hit
ACCGGGTGCTGTAGGCTTA389790.3007351612554295No Hit
CCCATAAAGTAGAAAGCACTAC381690.2944857582277248No Hit
AACCGGGCGGAAACACCA381300.29418486104490943No Hit
GGAATACCGGGTGCTGTAGGCTT376110.2901806139197506No Hit
CATTGCACTTGTCTCGGTCTGA367110.28323683277785655No Hit
CGCGACCTCAGATCAGACGTGG330630.2550913732160462No Hit
CCCATAAAGTAGAAAGCACT324980.2507322217214127No Hit
TGTAAACATCCTACACTCTCAGCT323660.2497138004872683No Hit
CTCCCACTGCTTCACTTGACTAG308770.23822570035362364No Hit
CCCCCACTGCTAAATTTGACTGGCTT306400.23639717131959154No Hit
CGCGACCTCAGATCAGACGTGGCG290070.22379806620324386No Hit
AGCTCGGTCTGAGGCCCCTCAGT281360.21707802912036642No Hit
GCGTTCGCGCTTTCCCCTG277240.21389932041985496No Hit
TTCAAGTAATCCAGGATAGGCTT276260.2131432198066265No Hit
TACGCCTGTCTGAGCGTCGCT272000.20985649673279666No Hit
CCCCCCACTGCTAAATTTGACTGGCT271310.2093241401785848No Hit
CTACGCCTGTCTGAGCGTCGCT271090.20915440330622737No Hit
TGAGGTAGTAGTTTGTACAGTT262710.20268897153188606No Hit
TATTGCACTTGTCCCGGCCTGTT259500.2002123562579439No Hit
CCCCCCACTGCTAAATTTGACTGG252020.19444130259779197No Hit
TGAATACCGGGTGCTGTAGGCTTT246810.19042162484787334No Hit
ACGCCTGTCTGAGCGTCGCT245170.18915631361757265No Hit
CACCCGGCTGTGTGCACATGTGC237930.18357042745453792No Hit
TTCTCACTACTGCACTTGACTAG234530.18094722124537796No Hit
TGATGTAGTAGATTGTATAGTT232620.17947359658082043No Hit
TAGCTTATCAGACTGATGTTGACA230130.17755248379822977No Hit
CAACGGAATCCCAAAAGCAGCT213960.1650768236799602No Hit
ACTTGACTTGGAGTCAGAAGGC212980.16432072306673173No Hit
CAAAGAATTCTCCTTTTGGGCT210410.16233788778510203No Hit
TTCACAGTGGCTAAGTTCCG206580.1593829231436071No Hit
TGCAAGTAATCCAGGATAGGCT197100.15206880700747877No Hit
CACTAGATTGTGAGCTCCTGGA190220.14676067209011978No Hit
TATTGCACTTGTCCCGGCCTGTAG189120.14591198772833275No Hit
CTTCTCACTACTGCACTTGACTAG179980.13886019221312038No Hit
TGAGGTAGTAGTTTGTGCTGTT177450.13690821818101018No Hit
TTATAAAGCAATGAGACTGATT175030.13504111258507867No Hit
TTCAAGTAATCCAGGATAGGC172700.13324344480056613No Hit
CAAAGTGCTGTTCGTGCAGGT170570.1316000832636512No Hit
CAACACCAGTCGATGGGCTGT169100.13046593234380852No Hit
GACCCGCGGGCGCTCTCCAGTCCT168730.13018046578575287No Hit
ACCACAGGGTAGAACCACGGAC165810.12792759457082725No Hit
TGAATACCGGGTGCTGTAGGCTT165390.1276035514508722No Hit
CGCGACCTCAGATCAGACGTGGC159360.12295121808580323No Hit
AACATTCAACGCTGTCGGTGA159170.12280462715058547No Hit
CACTAGATTGTGAGCTCCTGGAA157160.12125384936222912No Hit
TGTAAACATCCCCGACTGGAAGC155650.1200888371928669No Hit
ACCTGGTGCTGTAGGCTT154990.1195796265757947No Hit
CAAAGAATTCTCCTTTTGGGCTT154230.11899326283492365No Hit
CTCCCACTGCTTCACTTGACTAGCCT152730.11783596597794131No Hit
TTCACAGTGGCTAAGTTCTG149140.11506616883356358No Hit
TAGCAGCACGTAAATATTGGC149120.11505073820880381No Hit
ATCCCGGACGAGCCCCCA148920.11489643196120618No Hit
AGCAGCATTGTACAGGGCTATGT143000.11032896703231589No Hit
AACATTCAACGCTGTCGGTGAGA137580.10614726772241972No Hit
CAAAGTGCTGTTCGTGCAGGTAG135980.10491281774163858No Hit
AGCAGCATTGTACAGGGCTATG132870.1025133555914952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)