FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12093478
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG9127617.547547529337714No Hit
CAACGGAATCCCAAAAGCAGCTG6381585.276877338347165No Hit
AACATTCAACGCTGTCGGTGAGT5364184.4355974352456755No Hit
TAGCAGCACGTAAATATTGGCG3708493.066520648567765No Hit
TTCAAGTAATCCAGGATAGGCT3333392.7563534658929383No Hit
AAGCTGCCAGTTGAAGAACTGT3302222.7305792427951663No Hit
CCCCCACTGCTAAATTTGACTG2195491.815433078887645No Hit
AACATTCAACGCTGTCGGTGAG2073681.7147093664866304No Hit
TAGCTTATCAGACTGATGTTGAC1955711.6171609192988152No Hit
GGAATACCGGGTGCTGTAGGCTTT1877181.5522250919049094No Hit
TATTGCACTTGTCCCGGCCTGTAA1679581.388831236142324No Hit
TAGCTTATCAGACTGATGTTGACT1661611.373971987214927No Hit
TATTGCACTTGTCCCGGCCTGTAT1655331.3687791055641727No Hit
GCCTGTCTGAGCGTCGCT1647871.3626104913739456No Hit
TAGCTTATCAGACTGATGTTGA1422581.1763199966130504No Hit
GGAATACCGGGTGCTGTAGGCTT1335911.1046532684807464No Hit
TATTGCACTTGTCCCGGCCTGT1101680.9109703593953699No Hit
TGTAAACATCCTTGACTGGAAGCT908980.7516282743475451No Hit
TGAATACCGGGTGCTGTAGGCTTT831140.6872630024216358No Hit
TATTGCACTTGTCCCGGCCTGTA787730.6513676214567885No Hit
TGAGGTAGTAGGTTGTATAGTT756840.6258249281141455No Hit
TCCTGTCTGAGCGTCGCT707540.5850591533717596No Hit
AGCAGCATTGTACAGGGCTATGA656670.5429951582166851No Hit
TGTAAACATCCCCGACTGGAAGCT617410.5105313789796451No Hit
CATAAAGTAGAAAGCACTACT598200.4946467839938188No Hit
TGAATACCGGGTGCTGTAGGCTT583740.48268992592536236No Hit
ACTGGACTTGGAGTCAGAAGGC571200.47232070046350605No Hit
CCCCCCACTGCTAAATTTGACTGGCT527050.4358134194315316No Hit
CCCCCACTGCTAAATTTGACTGGCTT523200.4326298852985055No Hit
CCCATAAAGTAGAAAGCACTAC503150.41605070104729175No Hit
TGAGGTAGTAGATTGTATAGTT494550.4089394299968958No Hit
AACATTCAACGCTGTCGGTGAGTT486930.40263851308945203No Hit
TTCAAGTAATTCAGGATAGGTT469990.38863096290413723No Hit
CAACGGAATCCCAAAAGCAGCTGT464010.38368614884816427No Hit
TGTAAACATCCTACACTCTCAGCT428660.3544555172631066No Hit
AACCGGGCGGAAACACCA426990.35307460765215765No Hit
CTCCCACTGCTTCACTTGACTAG363440.30052562215766215No Hit
TCGTACGACTCTTAGCGG331860.2744123733470223No Hit
GTCAGGATGGCCGAGCGGTCTAAGG321570.26590365484602524No Hit
CCCCCCACTGCTAAATTTGACTGG314740.26025598260483873No Hit
GGAATACCGGGTGCTGTAGGCTTA307790.2545090833257397No Hit
CATTGCACTTGTCTCGGTCTGA286490.23689628409627075No Hit
CGCCTGTCTGAGCGTCGCT280230.2317199402851686No Hit
CTTCTCACTACTGCACTTGACTAG279570.2311741915766498No Hit
TTCTCACTACTGCACTTGACTAG272750.22553478825528933No Hit
TGATGTAGTAGGTTGTATAGTT269740.22304584338765074No Hit
CAAAGAATTCTCCTTTTGGGCT269680.22299622986869452No Hit
AGCTCGGTCTGAGGCCCCTCAGT253360.20950135271259435No Hit
TTCAAGTAATCCAGGATAGGCTT249260.20611109558391721No Hit
CCCATAAAGTAGAAAGCACT247930.20501132924705368No Hit
TAGCTTATCAGACTGATGTTGACA238420.19714758649248795No Hit
CACTAGATTGTGAGCTCCTGGA230080.1902513073575691No Hit
CTACGCCTGTCTGAGCGTCGCT228160.18866367475096907No Hit
TATTGCACTTGTCCCGGCCTGTT227450.18807658144332012No Hit
GACCCGCGGGCGCTCTCCAGTCCT225170.18619126772298258No Hit
ACTTGACTTGGAGTCAGAAGGC217190.17959266970180127No Hit
CAAAGAATTCTCCTTTTGGGCTT216550.17906345883293456No Hit
TGTAAACATCCCCGACTGGAAGC214120.17705411131520643No Hit
CTCCCACTGCTTCACTTGACTAGCCT212490.17570627738356162No Hit
CACCCGGCTGTGTGCACATGTGC210560.17411037585713557No Hit
CAACGGAATCCCAAAAGCAGCT209060.1728700378832293No Hit
CGCGACCTCAGATCAGACGTGGCG207890.1719025742635824No Hit
TATTGCACTTGTCCCGGCCTGTAG206210.17051339573280738No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC199970.1653535897613573No Hit
TGAGGTAGTAGTTTGTACAGTT198660.16427036126414585No Hit
GGGCTACGCCTGTCTGAGCGTCGCT195170.16138450824485728No Hit
CACTAGATTGTGAGCTCCTGGAA191360.15823404979113537No Hit
CCCCCACTGCTAAATTTGACTGGCT183790.15197447748282175No Hit
AACATTCAACGCTGTCGGTGA179190.1481707743628425No Hit
GGAATACCGGGTGCTGTAGGCT178630.1477077148525842No Hit
TGATGTAGTAGATTGTATAGTT170400.1409023938357518No Hit
AACATTCAACGCTGTCGGTGAGA170160.1407039397599268No Hit
TGCAAGTAATCCAGGATAGGCT169540.1401912667307122No Hit
TTCAAGTAATCCAGGATAGGC162470.13434514041370066No Hit
TTCACAGTGGCTAAGTTCCG160550.13275750780710066No Hit
TGTAAACATCCTACACTCAGCT159330.13174869958832355No Hit
ACCGGGTGCTGTAGGCTT157310.13007837778346312No Hit
TGTCAGTTTGTCAAATACCCCAA156250.1292018722819027No Hit
TATTGCACTTGTCCCGGCCTGTTT154140.12745713019860785No Hit
ACTGGACTTGGAGTCAGAAGGCA150710.12462089069827556No Hit
ACCCCCCACTGCTAAATTTGACTG150180.12418263794749533No Hit
TGAGGTAGTAGTTTGTGCTGTT147200.1217184998393349No Hit
TACGCCTGTCTGAGCGTCGCT141400.11692252634023066No Hit
TGAATACCGGGTGCTGTAGGCTTA141030.11661657630666711No Hit
CGCGACCTCAGATCAGACGTGG138520.11454107743033062No Hit
CGCCCCACTGCTAAATTTGACTG136540.11290383130477435No Hit
TAGCAGCACGTAAATATTGGC136100.11253999883242853No Hit
TAGCAGCACGTAAATATTGGCGT135600.11212655284112644No Hit
CGCGACCTCAGATCAGACGTGGC135330.1119032920058233No Hit
TTCAGGATGGCCGAGCGGTCTAAGG131270.10854611055645033No Hit
CTCCCCACTGCTAAATTTGACTG128570.10631350220341906No Hit
AGCAGCATTGTACAGGGCTATGT127930.1057842913345524No Hit
GCGTTCGCGCTTTCCCCTG127920.10577602241472635No Hit
TTAATGCTAATCGTGATAGGGGTT126410.10452741552099405No Hit
TGTCAGTTTGTCAAATACCCCA121030.10007873665458357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)