FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7679457
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7418109.659667343667659No Hit
CAACGGAATCCCAAAAGCAGCTG4398125.727123675541122No Hit
AAGCTGCCAGTTGAAGAACTGT2910663.790189853266969No Hit
TTCAAGTAATCCAGGATAGGCT2497523.2522091080137567No Hit
TAGCAGCACGTAAATATTGGCG2345433.054161251244717No Hit
CCCCCACTGCTAAATTTGACTG2293842.986982022296629No Hit
AACATTCAACGCTGTCGGTGAGT2063842.6874816800198245No Hit
TAGCTTATCAGACTGATGTTGAC1875412.4421127691710494No Hit
GCCTGTCTGAGCGTCGCT1559242.03040397257254No Hit
TAGCTTATCAGACTGATGTTGACT1423621.8538029446613216No Hit
AACATTCAACGCTGTCGGTGAG1110971.4466778054750484No Hit
TATTGCACTTGTCCCGGCCTGTAT1033631.3459675599459702No Hit
TGTAAACATCCTTGACTGGAAGCT982011.2787492657358457No Hit
TATTGCACTTGTCCCGGCCTGTAA913521.1895632725074181No Hit
TAGCTTATCAGACTGATGTTGA852051.1095185505954392No Hit
TGAGGTAGTAGGTTGTATAGTT749140.9755116800575874No Hit
TATTGCACTTGTCCCGGCCTGT682940.889307668497916No Hit
AGCAGCATTGTACAGGGCTATGA610160.7945353427983255No Hit
CATAAAGTAGAAAGCACTACT539200.7021329763289254No Hit
TTCAAGTAATTCAGGATAGGTT516850.6730293561120272No Hit
AACCGGGCGGAAACACCA479850.6248488662674978No Hit
TGTAAACATCCCCGACTGGAAGCT478780.6234555385882101No Hit
TGAGGTAGTAGATTGTATAGTT473720.6168665310581204No Hit
ACTGGACTTGGAGTCAGAAGGC430050.5600005312875637No Hit
TATTGCACTTGTCCCGGCCTGTA426570.5554689608913755No Hit
CTCCCACTGCTTCACTTGACTAG412690.537394766322671No Hit
TTCTCACTACTGCACTTGACTAG395900.5155312413364642No Hit
TGTAAACATCCTACACTCTCAGCT393340.5121976723093833No Hit
CAACGGAATCCCAAAAGCAGCTGT349410.4549931069345137No Hit
CCCATAAAGTAGAAAGCACTAC342400.4458648573720772No Hit
CTTCTCACTACTGCACTTGACTAG322820.42036826301651276No Hit
TCCTGTCTGAGCGTCGCT312480.4069037693680686No Hit
CCCCCACTGCTAAATTTGACTGGCTT292360.3807040002958543No Hit
CGCCTGTCTGAGCGTCGCT290540.3783340410656639No Hit
CCCCCCACTGCTAAATTTGACTGGCT260580.33932086604560713No Hit
TCGTACGACTCTTAGCGG239160.31142826895182824No Hit
TAGCTTATCAGACTGATGTTGACA236240.30762591678031403No Hit
GGAATACCGGGTGCTGTAGGCTTT234830.3057898494646171No Hit
CATTGCACTTGTCTCGGTCTGA229170.29841953669380533No Hit
CCCCCCACTGCTAAATTTGACTGG225700.2939009880516292No Hit
TGAGGTAGTAGTTTGTACAGTT197770.2575312290960155No Hit
TTCAAGTAATCCAGGATAGGCTT191040.248767588645916No Hit
CCCATAAAGTAGAAAGCACT190820.2484811100576512No Hit
AACATTCAACGCTGTCGGTGAGTT175940.2291047400877432No Hit
CAAAGAATTCTCCTTTTGGGCT175470.22849271764917756No Hit
TGTCAGTTTGTCAAATACCCCA171590.22344027709250797No Hit
TGTAAACATCCTACACTCAGCT169590.22083592629010101No Hit
AGCTCGGTCTGAGGCCCCTCAGT168830.21984627298518633No Hit
ACCCCCCACTGCTAAATTTGACTG168390.21927331580865678No Hit
CTCCCACTGCTTCACTTGACTAGCCT161320.21006693572214805No Hit
GGAATACCGGGTGCTGTAGGCTT159720.20798345508022245No Hit
TGTCAGTTTGTCAAATACCCCAA158270.2060953007484774No Hit
CTACGCCTGTCTGAGCGTCGCT150750.1963029417314271No Hit
AGCAGCATTGTACAGGGCTATGT145420.18936234684301245No Hit
CAACGGAATCCCAAAAGCAGCT137380.17889285661733637No Hit
TATTGCACTTGTCCCGGCCTGTT135520.17647081037109785No Hit
CAAAGAATTCTCCTTTTGGGCTT133890.17434826446713617No Hit
TACGCCTGTCTGAGCGTCGCT133030.17322839362210116No Hit
TGTAAACATCCCCGACTGGAAGC132690.17278565398569196No Hit
TAGCAGCACGTAAATATTGGC128750.16765508290495018No Hit
CCCCCCACTGCTAAATTTGACTGT127030.16541534121488016No Hit
CAACACCAGTCGATGGGCTGT118170.15387806716021718No Hit
CCCCCCACTGCTAAATTTGACTGTA115880.15089608549146116No Hit
TCCCACTGCTTCACTTGACTAG115620.15055751988714827No Hit
TGAGGTAGTAGTTTGTGCTGTT115350.15020593252882333No Hit
TATTGCACTTGTCCCGGCCTGTAG111040.14459355654963627No Hit
TTATAAAGCAATGAGACTGATT110690.14413779515921504No Hit
TTCAAGTAATCCAGGATAGGC110390.14374714253885398No Hit
CACTAGATTGTGAGCTCCTGGAA109720.14287468502004766No Hit
CACTAGATTGTGAGCTCCTGGA106930.13924161565068988No Hit
ATCACATTGCCAGGGATTTCCA106840.13912441986458157No Hit
TGTCAGTTTGTCAAATACCCC106750.13900722407847327No Hit
AACGGAATCCCAAAAGCAGCTG105780.13774411393930586No Hit
CGCGACCTCAGATCAGACGTGGCG105610.13752274412110127No Hit
TAGCAGCACGTAAATATTGGCGT105000.13672841712636713No Hit
TATTGCACTTGTCCCGGCCTGTTT104550.13614243819582555No Hit
CAAAGTGCTGTTCGTGCAGGTAG98930.1288242124410619No Hit
GGGCTACGCCTGTCTGAGCGTCGCT98260.12795175492225558No Hit
CACCCGGCTGTGTGCACATGTGC97890.12746995002381026No Hit
TTCACAGTGGCTAAGTTCCG94680.12328996698594706No Hit
GCGTTCGCGCTTTCCCCTG93290.12147994317827418No Hit
TGTAAACATCCTTGACTGGAAGC92870.12093302950976871No Hit
TATTGCACTTGTCCCGGCCTGTAAT89400.1164144808675926No Hit
ATCACATTGCCAGGGATTTCC88960.11584152369106306No Hit
ACCGGGTGCTGTAGGCTT87990.11457841355189567No Hit
TGATGTAGTAGGTTGTATAGTT85460.11128390978685082No Hit
TCGTTTCCCGGCCAATGCACCA85410.11121880101679063No Hit
ACCACAGGGTAGAACCACGGAC85070.11077606138038146No Hit
TTCACAGTGGCTAAGTTCTGC84540.11008590841774359No Hit
CCCCCACTGCTAAATTTGACTGGCT83840.10917438563690116No Hit
ACGCCTGTCTGAGCGTCGCT83070.10817171057797446No Hit
AGCAGCATTGTACAGGGCTATG82650.107624796909469No Hit
TGAGGTAGTAGGTTGTGTGGTT81270.10582779485580818No Hit
CAAAGTGCTGTTCGTGCAGGT77750.10124413744357186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)