FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7835442
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT96217512.279779494251889No Hit
CAACGGAATCCCAAAAGCAGCTG4913996.27149049153832No Hit
CCCCCCACTGCTAAATTTGACTG3147234.01665917506632No Hit
TAGCAGCACGTAAATATTGGCG2605243.3249432514464403No Hit
AAGCTGCCAGTTGAAGAACTGT2504003.1957354798874142No Hit
TTCAAGTAATCCAGGATAGGCT2435203.1079293293218173No Hit
CCCCCACTGCTAAATTTGACTG1808512.308114845339931No Hit
TAGCTTATCAGACTGATGTTGAC1603652.0466618220133594No Hit
AACATTCAACGCTGTCGGTGAGT1526871.9486711789839042No Hit
CATAAAGTAGAAAGCACTACT1459201.8623071933912598No Hit
TAGCTTATCAGACTGATGTTGA1383001.7650567766311076No Hit
AACATTCAACGCTGTCGGTGAG1272221.6236735591942355No Hit
TGAGGTAGTAGGTTGTATAGTT978371.2486468536171922No Hit
TAGCTTATCAGACTGATGTTGACT850251.0851334232325376No Hit
TATTGCACTTGTCCCGGCCTGT844791.0781650862835817No Hit
TCGTACGACTCTTAGCGG773550.9872448803781587No Hit
TTCAAGTAATTCAGGATAGGTT606090.7735236888997455No Hit
TGAGGTAGTAGATTGTATAGTT557060.7109490440998734No Hit
TGTAAACATCCTTGACTGGAAGCT521580.6656676164535453No Hit
TATTGCACTTGTCCCGGCCTGTAT505240.6448136556942161No Hit
CATTGCACTTGTCTCGGTCTGA462090.5897433737624502No Hit
TATTGCACTTGTCCCGGCCTGTAA387970.49514756155428113No Hit
CAACGGAATCCCAAAAGCAGCT385470.49195693108314753No Hit
AGCAGCATTGTACAGGGCTATGA374250.4776373815287No Hit
TATTGCACTTGTCCCGGCCTGTA367860.4694821300444825No Hit
ACCGGGTGCTGTAGGCTT342220.4367590239325363No Hit
CCCATAAAGTAGAAAGCACT341140.43538067156900656No Hit
TTCTCACTACTGCACTTGACTAG323930.4134163714057229No Hit
AACCGGGCGGAAACACCA303410.3872276764986583No Hit
CAACACCAGTCGATGGGCTGT300070.3829649941892238No Hit
CTCCCACTGCTTCACTTGACTAG298440.3808847031220447No Hit
CCCATAAAGTAGAAAGCACTAC274200.3499483500739333No Hit
CAACGGAATCCCAAAAGCAGCTGT268390.3425333248590188No Hit
CGCCTGTCTGAGCGTCGCT260870.33293590840184895No Hit
TGTAAACATCCCCGACTGGAAGCT255730.32637597215319825No Hit
TGAGAACTGAATTCCATGGGTT246420.3144940642786967No Hit
ACTGGACTTGGAGTCAGAAGGC239040.3050753231279103No Hit
ACCGGGTGCTGTAGGCTTT227860.29080682366100086No Hit
TGAGGTAGTAGTTTGTACAGTT227520.29037289791692666No Hit
TAGCAGCACGTAAATATTGGC209310.26713234556518956No Hit
TCCTGTCTGAGCGTCGCT199990.2552376751688035No Hit
CTTCTCACTACTGCACTTGACTAG194000.24759292455996737No Hit
TTAATGCTAATCGTGATAGGGGTT185840.2371787067021873No Hit
TGAGGTAGTAGTTTGTGCTGTT177930.2270835518915206No Hit
ATCCCGGACGAGCCCCCA160590.20495333894373796No Hit
TTATAAAGCAATGAGACTGATT153480.195879185883834No Hit
TGTAAACATCCTACACTCTCAGCT149270.19050616417044502No Hit
TAGTGGGTTATCAGAACT149130.19032748886406153No Hit
TAGCTTATCAGACTGATGTTGACA145330.18547773054793845No Hit
TGATGTAGTAGGTTGTATAGTT143970.18374202757164176No Hit
TTCACAGTGGCTAAGTTCCGC143710.18341020200264388No Hit
AACGGAATCCCAAAAGCAGCTG143080.18260616312391822No Hit
ACCGGGTGCTGTAGGCTTA139290.17776916732967968No Hit
CCCCCACTGCTAAATTTGACTGGCTT136940.1747699746868141No Hit
TGTCAGTTTGTCAAATACCCCA136800.1745912993804306No Hit
TATTGCACTTGTCCCGGCCTGTT133600.1705072923773796No Hit
TTCACAGTGGCTAAGTTCCG132960.16969049097676941No Hit
TCGTTTCCCGGCCAATGCACCA131610.16796755052235726No Hit
TTCACAGTGGCTAAGTTCTGC129240.1649428328357226No Hit
CACTAGATTGTGAGCTCCTGGA127450.16265834141839094No Hit
TGTCAGTTTGTCAAATACCCCAA120660.1539925890587921No Hit
TTCAAGTAATCCAGGATAGGCTT119770.15285672461106853No Hit
TCCCACTGCTTCACTTGACTAG117780.15031698275604619No Hit
TTCAAGTAATCCAGGATAGGC117520.14998515718704827No Hit
TGAGAACTGAATTCCATAGGCTGT117220.14960228153051225No Hit
CACCCGGCTGTGTGCACATGTGC116130.148211166645098No Hit
CTACGCCTGTCTGAGCGTCGCT115150.14696043950041365No Hit
CCCATAAAGTAGAAAGCACTA114180.14572247487761378No Hit
TAGCAGCACGTAAATATTGGCGT104030.1327685151648114No Hit
TGTAAACATCCTACACTCAGCT103740.13239840203015987No Hit
TGAGGTAGTAGGTTGTGTGGTT99460.12693604266357916No Hit
AGAATTACTCTGAGACCT97990.1250599519465526No Hit
AACATTCAACGCTGTCGGTGAGTT97330.12421762550217333No Hit
AGCAGCATTGTACAGGGCTATG96610.12329872392648686No Hit
CCCCCCACTGCTAAATTTGACTGG93460.11927852953285852No Hit
TGTAAACATCCCCGACTGGAAGC92920.11858935335109365No Hit
TGAGGTAGTAGGTTGTATAGT92910.11857659082920911No Hit
AATGACACGATCACTCCCGTTG90950.11607513653984038No Hit
CTCCCACTGCTTCACTTGACTAGCCT89570.11431390851977463No Hit
TTCACAGTGGCTAAGTTCTG89260.11391827034135406No Hit
ATCCCACCGCTGCCACCA88830.11336948190031909No Hit
AGCTCGGTCTGAGGCCCCTCAGT88190.11255268049970889No Hit
CATTATTACTTTTGGTACGCG87470.11163377892402242No Hit
AATGCACCCGGGCAAGGATTCT84990.10846867349665788No Hit
CAAAGAATTCTCCTTTTGGGCT83920.10710308365501271No Hit
AGCAGCATTGTACAGGGCTATGT83730.10686059573920655No Hit
TGATGTAGTAGATTGTATAGTT83330.10635009486382517No Hit
CACTAGATTGTGAGCTCCTGGAA81290.10374654039938015No Hit
TAGCTTATCAGACTGATGTTG80820.10314670187080703No Hit
ACCACAGGGTAGAACCACGGAC79310.10121956106624234No Hit
CATAAAGTAGAAAGCACTAC78640.10036447209997854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)