FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7778292
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT7033879.042949274725094No Hit
CAACGGAATCCCAAAAGCAGCTG4774056.137658498806679No Hit
CCCCCCACTGCTAAATTTGACTG3853354.953979614033518No Hit
TTCAAGTAATCCAGGATAGGCT3185954.095950627721356No Hit
AAGCTGCCAGTTGAAGAACTGT3184894.094587860676868No Hit
TAGCAGCACGTAAATATTGGCG2798343.5976278596895046No Hit
TCCTGTCTGAGCGTCGCT1948092.5045215582032663No Hit
CCCCCACTGCTAAATTTGACTG1900772.4436855803304893No Hit
AACATTCAACGCTGTCGGTGAGT1408131.8103331682585329No Hit
TAGCTTATCAGACTGATGTTGAC1301891.673747912780852No Hit
CATAAAGTAGAAAGCACTACT1276561.6411829229347523No Hit
TGAGGTAGTAGGTTGTATAGTT1142521.4688571732714586No Hit
AACATTCAACGCTGTCGGTGAG1032011.3267822807372107No Hit
TAGCTTATCAGACTGATGTTGACT993711.277542679035449No Hit
TAGCTTATCAGACTGATGTTGA975331.2539128127357522No Hit
TATTGCACTTGTCCCGGCCTGT629540.8093550614967913No Hit
TGTAAACATCCTTGACTGGAAGCT626220.8050867722631139No Hit
TGAGGTAGTAGATTGTATAGTT596740.7671864208749171No Hit
TATTGCACTTGTCCCGGCCTGTAT559410.7191938795817899No Hit
TATTGCACTTGTCCCGGCCTGTAA538210.6919385386920419No Hit
AACCGGGCGGAAACACCA482390.6201747118776204No Hit
TTCAAGTAATTCAGGATAGGTT476620.6127566308901748No Hit
CCCATAAAGTAGAAAGCACTAC439120.5645455326182148No Hit
AGCAGCATTGTACAGGGCTATGA366880.47167167290711126No Hit
CAACGGAATCCCAAAAGCAGCTGT355300.45678408576072993No Hit
TTCTCACTACTGCACTTGACTAG339890.43697253844417255No Hit
CGCCTGTCTGAGCGTCGCT323950.41647960760537145No Hit
TATTGCACTTGTCCCGGCCTGTA322600.41474400806758094No Hit
CATTGCACTTGTCTCGGTCTGA282320.36295886037705966No Hit
ACTGGACTTGGAGTCAGAAGGC281210.36153181186820965No Hit
TGAGGTAGTAGTTTGTACAGTT261030.33558781285145894No Hit
CCCATAAAGTAGAAAGCACT256900.330278163895107No Hit
CTACGCCTGTCTGAGCGTCGCT252320.3243899817594917No Hit
TGTAAACATCCCCGACTGGAAGCT250750.32237154377850563No Hit
CTCCCACTGCTTCACTTGACTAG247060.3176275717085448No Hit
TTAATGCTAATCGTGATAGGGGTT235730.30306139188397657No Hit
TGTAAACATCCTACACTCTCAGCT230240.2960032870969616No Hit
CAACACCAGTCGATGGGCTGT210540.270676390138092No Hit
TAGCAGCACGTAAATATTGGC206520.2655081604033379No Hit
TTCACAGTGGCTAAGTTCCGC204260.26260263821414775No Hit
CCCCCACTGCTAAATTTGACTGGCTT195300.2510833998003675No Hit
CCCTGTCTGAGCGTCGCT190810.24531092430060483No Hit
CTTCTCACTACTGCACTTGACTAG189710.24389673208462734No Hit
TACGCCTGTCTGAGCGTCGCT164500.2114860177529977No Hit
TAGCAGCACATCATGGTTTACA162260.2086062081495526No Hit
ACCGGGTGCTGTAGGCTT153610.19748551481482052No Hit
TGTAAACATCCTACACTCAGCT148630.19108308096430424No Hit
TGAGGTAGTAGTTTGTGCTGTT146460.18829326541096683No Hit
TTCAAGTAATCCAGGATAGGC146180.18793328921053618No Hit
TAGCTTATCAGACTGATGTTGACA145000.18641624665157852No Hit
TGTCAGTTTGTCAAATACCCCA142760.18353643704813347No Hit
TTCAAGTAATCCAGGATAGGCTT141340.18171084346023525No Hit
TTCACAGTGGCTAAGTTCCG141310.18167227458161767No Hit
TTCACAGTGGCTAAGTTCTGC138880.17854819541359465No Hit
CCCCCCACTGCTAAATTTGACTGG138220.17769968008400816No Hit
TAGCAGCACGTAAATATTGGCGT130960.16836601145855673No Hit
AACGGAATCCCAAAAGCAGCTG129400.16636042977044319No Hit
TCGTTTCCCGGCCAATGCACCA124780.16042082246333772No Hit
TCCCACTGCTTCACTTGACTAG124450.15999656479854446No Hit
CAAAGAATTCTCCTTTTGGGCT122590.15760529432425524No Hit
ACCGGGTGCTGTAGGCTTT122430.1573995936382949No Hit
ACGCCTGTCTGAGCGTCGCT122040.15689819821626652No Hit
TTATAAAGCAATGAGACTGATT118330.1521285135605606No Hit
TGTCAGTTTGTCAAATACCCCAA118300.15208994468194303No Hit
ATCACATTGCCAGGGATTTCC116500.14977581196488893No Hit
AGCTCGGTCTGAGGCCCCTCAGT115370.14832305087029388No Hit
CCCCCCACTGCTAAATTTGACTGGCT111680.14357907880033302No Hit
TATTGCACTTGTCCCGGCCTGTT110030.14145779047636678No Hit
TGAGAACTGAATTCCATGGGTT109310.14053213738954518No Hit
CAACGGAATCCCAAAAGCAGCT107150.13775517812908028No Hit
TGAGGTAGTAGGTTGTATAGT104800.13473394930403745No Hit
ACCCCCCACTGCTAAATTTGACTG103870.13353831406689284No Hit
TGAGGTAGTAGGTTGTGTGGTT101050.12991283947684146No Hit
TAGCAGCACATAATGGTTTGT99760.12825437769628603No Hit
TCCACCACGTTCCCGTGG99170.1274958564168072No Hit
GCCTGTCTGAGCGTCGCG98130.1261588019580648No Hit
AACATTCAACGCTGTCGGTGAGTT97510.12536171179996844No Hit
GCCTGTCTGAGCGTCGCA94680.12172338091704452No Hit
ACCGGGTGCTGTAGGCTTA92630.11908784087817736No Hit
AGAGGTAGTAGGTTGCATAGTT89580.11516667155205797No Hit
CTCCCACTGCTTCACTTGACTAGCCT89380.11490954569460751No Hit
CCCATAAAGTAGAAAGCACTA86940.11177261023371197No Hit
CACCCGGCTGTGTGCACATGTGC85990.11055126241082233No Hit
GTCAGGATGGCCGAGCGGTCTAAGG84160.10819856081515067No Hit
CAAAGAATTCTCCTTTTGGGCTT82990.10669437454906552No Hit
TGAGAACTGAATTCCATAGGCTGT82170.10564015853351869No Hit
TGTAAACATCCCCGACTGGAAGC81510.10479164320393217No Hit
AGCAGCATTGTACAGGGCTATGT79850.10265749858709342No Hit
CATAAAGTAGAAAGCACTAC78090.10039479104152942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)