FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10561734
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9111148.626556964983212No Hit
CCCCCCACTGCTAAATTTGACTG8758218.292397820282162No Hit
TTCAAGTAATCCAGGATAGGCT4320414.090625649159504No Hit
TAGCAGCACGTAAATATTGGCG4077373.8605119197283324No Hit
CCCCCACTGCTAAATTTGACTG3332963.1556939419227943No Hit
AAGCTGCCAGTTGAAGAACTGT3209493.038790789466957No Hit
AACATTCAACGCTGTCGGTGAGT2061821.952160506977358No Hit
TAGCTTATCAGACTGATGTTGAC2054101.9448511011544127No Hit
GCCTGTCTGAGCGTCGCT1878131.778240201845644No Hit
AACATTCAACGCTGTCGGTGAG1876301.7765075318124843No Hit
TAGCTTATCAGACTGATGTTGACT1762921.669157734894668No Hit
TGAGGTAGTAGGTTGTATAGTT1436701.360287998163938No Hit
TATTGCACTTGTCCCGGCCTGTAT1332431.261563678842887No Hit
TAGCTTATCAGACTGATGTTGA1310611.2409041924365827No Hit
TATTGCACTTGTCCCGGCCTGTAA1276731.2088261264674909No Hit
CATAAAGTAGAAAGCACTACT1157051.0955113999273225No Hit
TGTAAACATCCTTGACTGGAAGCT1102401.0437680024889853No Hit
TGAGGTAGTAGATTGTATAGTT904310.8562135725061812No Hit
TTCAAGTAATTCAGGATAGGTT848580.8034476157040122No Hit
TATTGCACTTGTCCCGGCCTGT754120.7140115439377662No Hit
CCCATAAAGTAGAAAGCACTAC667330.6318375372831772No Hit
CAACGGAATCCCAAAAGCAGCTGT617740.5848850198272367No Hit
AGCAGCATTGTACAGGGCTATGA616820.5840139507395281No Hit
TTCTCACTACTGCACTTGACTAG606350.574100805795715No Hit
CTCCCACTGCTTCACTTGACTAG587880.5566131470457407No Hit
CCCCCACTGCTAAATTTGACTGGCTT562690.5327628966985913No Hit
TATTGCACTTGTCCCGGCCTGTA561790.5319107638953983No Hit
TGTAAACATCCCCGACTGGAAGCT455460.4312360072692609No Hit
CTTCTCACTACTGCACTTGACTAG421800.3993662404298385No Hit
ACTGGACTTGGAGTCAGAAGGC403160.3817176232614834No Hit
CATTGCACTTGTCTCGGTCTGA377930.35782950034530314No Hit
TGTAAACATCCTACACTCTCAGCT328750.31126517672192844No Hit
TGAGGTAGTAGTTTGTACAGTT326020.308680373885576No Hit
TAGCTTATCAGACTGATGTTGACA315170.29840743953597015No Hit
CAAAGAATTCTCCTTTTGGGCT313900.29720498546924207No Hit
ACCCCCCACTGCTAAATTTGACTG311500.2949326313273938No Hit
TAGCAGCACGTAAATATTGGC303860.28769897064251004No Hit
TCGTACGACTCTTAGCGG282940.267891617039399No Hit
CCCATAAAGTAGAAAGCACT273760.2591998624468293No Hit
CCCCCCACTGCTAAATTTGACTGGCT270300.25592388522566467No Hit
CTCCCACTGCTTCACTTGACTAGCCT250380.23706334584832375No Hit
TGATGTAGTAGGTTGTATAGTT240570.22777509829351883No Hit
CAACGGAATCCCAAAAGCAGCT239130.22641168580840987No Hit
GGAATACCGGGTGCTGTAGGCTTT238680.22598561940681333No Hit
TGTCAGTTTGTCAAATACCCCA233380.22096750401023163No Hit
CCCCCCACTGCTAAATTTGACTGG223080.21121531748479935No Hit
TTCAAGTAATCCAGGATAGGCTT212880.2015578123819441No Hit
TTCAAGTAATCCAGGATAGGC211890.2006204662984317No Hit
CAACACCAGTCGATGGGCTGT209960.19879311484269535No Hit
AACGGAATCCCAAAAGCAGCTG199870.1892397593046748No Hit
AACCGGGCGGAAACACCA187730.17774543460382547No Hit
TGTCAGTTTGTCAAATACCCCAA185960.17606957342421237No Hit
TCCCACTGCTTCACTTGACTAG184530.17471562908136107No Hit
TGAGGTAGTAGTTTGTGCTGTT178950.1694324057015638No Hit
ATCACATTGCCAGGGATTTCC178060.16858974104062835No Hit
CAAAGAATTCTCCTTTTGGGCTT173400.16417758674853958No Hit
GGAATACCGGGTGCTGTAGGCTT170890.16180108304185659No Hit
GTCAGGATGGCCGAGCGGTCTAAGG169170.16017256257353196No Hit
TTCACAGTGGCTAAGTTCTGC168370.15941511119291588No Hit
TTCACAGTGGCTAAGTTCCGC165940.15711435262429446No Hit
TTCACAGTGGCTAAGTTCCG165450.15665041365366708No Hit
TATTGCACTTGTCCCGGCCTGTT163170.15449167721891122No Hit
CACTAGATTGTGAGCTCCTGGAA158850.15040143976358428No Hit
CACCCGGCTGTGTGCACATGTGC157560.1491800494123408No Hit
CACTAGATTGTGAGCTCCTGGA157030.14867823787268264No Hit
CCCATAAAGTAGAAAGCACTA154410.14619758460116494No Hit
AGCTCGGTCTGAGGCCCCTCAGT152630.1445122552792941No Hit
TCGTTTCCCGGCCAATGCACCA149820.14185170730488006No Hit
TGATGTAGTAGATTGTATAGTT148620.1407155302339559No Hit
TATTGCACTTGTCCCGGCCTGTAG147700.1398444611462474No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC146210.13843370794984988No Hit
TAGCAGCACGTAAATATTGGCGT145140.13742061672827585No Hit
TGTAAACATCCCCGACTGGAAGC142910.1353092210048085No Hit
AACATTCAACGCTGTCGGTGAGTT140650.133169420854568No Hit
AGCAGCATTGTACAGGGCTATGT137650.13032897817725764No Hit
TTATAAAGCAATGAGACTGATT135390.12818917802701715No Hit
TCCTGTCTGAGCGTCGCT127660.1208703040618141No Hit
ATCACATTGCCAGGGATTTCCA127300.12052945094053685No Hit
TGTAAACATCCTACACTCAGCT125600.11891986675672764No Hit
TAGCAGCACATCATGGTTTACA125090.11843699150158486No Hit
TGAGGTAGTAGGTTGTATAGT121250.11480122487462759No Hit
TATTGCACTTGTCCCGGCCTGTTT120340.11393962392917678No Hit
TATTGCACTTGTCCCGGCCTGTAAT115390.10925289351161466No Hit
ATGCACCTGGGCAAGGATTCTGA110430.10455669495179484No Hit
CTACGCCTGTCTGAGCGTCGCT109230.1034205178808707No Hit
CAAAGTGCTGTTCGTGCAGGTAG109190.1033826453118399No Hit
GTGCGGTAACGCGACCGATCCCGGA107930.10218965938736954No Hit
TGTCAGTTTGTCAAATACCCC107150.10145114429126884No Hit
TTAATGCTAATCGTGATAGGGGTT106700.10102507788967229No Hit
GACCCGCGGGCGCTCTCCAGTCCT106400.10074103362194124No Hit
CGCGACCTCAGATCAGACGTGGCG106220.10057060706130261No Hit
TTCACAGTGGCTAAGTTCTG105990.1003528397893755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)