FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6124888
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5296828.648027523115525No Hit
CCCCCCACTGCTAAATTTGACTG3050334.980221679155602No Hit
AAGCTGCCAGTTGAAGAACTGT2463914.022783763556166No Hit
AACATTCAACGCTGTCGGTGAGT2405223.926961603216255No Hit
TTCAAGTAATCCAGGATAGGCT2205803.601371976107971No Hit
TAGCAGCACGTAAATATTGGCG1837092.9993854581504187No Hit
AACATTCAACGCTGTCGGTGAG1404582.2932337701522054No Hit
CCCCCACTGCTAAATTTGACTG1088451.7770937199178172No Hit
TAGCTTATCAGACTGATGTTGAC895341.4618063220094801No Hit
GCCTGTCTGAGCGTCGCT871801.4233729661668915No Hit
TAGCTTATCAGACTGATGTTGACT799331.30505243524453No Hit
CGCCTGTCTGAGCGTCGCT789691.2893133719343113No Hit
TATTGCACTTGTCCCGGCCTGT716261.169425465412592No Hit
TCGTACGACTCTTAGCGG714831.167090728842715No Hit
TATTGCACTTGTCCCGGCCTGTAA710471.1599722313289647No Hit
TATTGCACTTGTCCCGGCCTGTAT688791.1245756657101322No Hit
TAGCTTATCAGACTGATGTTGA673351.099367041487126No Hit
TGAGGTAGTAGGTTGTATAGTT628781.0265983639211036No Hit
CATAAAGTAGAAAGCACTACT571420.932947671859469No Hit
TTCAAGTAATTCAGGATAGGTT461320.7531892828081101No Hit
TGTAAACATCCTTGACTGGAAGCT445490.7273439122478648No Hit
AGCAGCATTGTACAGGGCTATGA414470.6766980881936127No Hit
TCCTGTCTGAGCGTCGCT379180.6190807080880499No Hit
TGAGGTAGTAGATTGTATAGTT378930.6186725373590505No Hit
AACCGGGCGGAAACACCA366070.5976762350593187No Hit
CCCATAAAGTAGAAAGCACT363800.5939700448400036No Hit
TATTGCACTTGTCCCGGCCTGTA362700.592174093632406No Hit
CAACGGAATCCCAAAAGCAGCTGT347900.5680103864756384No Hit
CCCATAAAGTAGAAAGCACTAC340970.5566958938677735No Hit
ACTGGACTTGGAGTCAGAAGGC318460.5199442014286628No Hit
CATTGCACTTGTCTCGGTCTGA255270.4167749679667612No Hit
TGTAAACATCCCCGACTGGAAGCT237620.3879581144993998No Hit
TACGCCTGTCTGAGCGTCGCT231130.377362002374574No Hit
CAACACCAGTCGATGGGCTGT223240.3644801341673513No Hit
CTACGCCTGTCTGAGCGTCGCT203970.3330183343760735No Hit
TGTAAACATCCTACACTCTCAGCT196220.32036504177709046No Hit
CTCCCACTGCTTCACTTGACTAG183090.2989279150900392No Hit
TGAGGTAGTAGTTTGTACAGTT175850.28710729077821506No Hit
AACATTCAACGCTGTCGGTGAGTT174320.2846092859167384No Hit
ACGCCTGTCTGAGCGTCGCT171780.280462271310104No Hit
CAACGGAATCCCAAAAGCAGCT141840.2315797448051295No Hit
TTCTCACTACTGCACTTGACTAG136120.22224079852562204No Hit
CACCCGGCTGTGTGCACATGTGC131680.21499168637859173No Hit
TTCAAGTAATCCAGGATAGGCTT129140.2108446717719573No Hit
AACGGAATCCCAAAAGCAGCTG128040.2090487205643597No Hit
AGCTCGGTCTGAGGCCCCTCAGT123230.20119551573841024No Hit
CCCCCCACTGCTAAATTTGACTGGCT118470.19342394505826066No Hit
TGAGGTAGTAGTTTGTGCTGTT118100.19281985237934146No Hit
TTCAAGTAATCCAGGATAGGC116120.1895871402056658No Hit
ATCCCACCGCTGCCACCA114330.1866646377860297No Hit
TATTGCACTTGTCCCGGCCTGTT113370.18509726218667183No Hit
TAGCAGCACGTAAATATTGGC109770.17921960368907972No Hit
ACCACAGGGTAGAACCACGGAC108360.1769175207775228No Hit
CCCCCACTGCTAAATTTGACTGGCTT107860.1761011793195239No Hit
TGAGGTAGTAGGTTGTGTGGTT107670.1757909695654843No Hit
CTTCTCACTACTGCACTTGACTAG105540.17231335495440897No Hit
ACCGGGTGCTGTAGGCTT98840.16137437941722363No Hit
AGCAGCATTGTACAGGGCTATGT98520.16085192088410433No Hit
CACTAGATTGTGAGCTCCTGGA98380.16062334527586464No Hit
GCGTTCGCGCTTTCCCCTG97000.15837024285178766No Hit
TTCACAGTGGCTAAGTTCCG96020.1567702135941098No Hit
TTATAAAGCAATGAGACTGATT95360.15569264286955123No Hit
ATCCCGGACGAGCCCCCA93020.15187216484611638No Hit
TAGCTTATCAGACTGATGTTGACA91810.14989661851775904No Hit
CCCCCCACTGCTAAATTTGACTGG89540.146190428298444No Hit
CAAAGTGCTGTTCGTGCAGGT85490.13957806248865284No Hit
CAAAGAATTCTCCTTTTGGGCT82460.1346310332531795No Hit
TGAGGTAGTAGGTTGTATAGT81290.13272079424146205No Hit
TGTAAACATCCTACACTCAGCT77450.12645129184403045No Hit
TCGTTTCCCGGCCAATGCACCA77410.12638598452739053No Hit
ACCGGGTGCTGTAGGCTTT76990.12570025770267146No Hit
TATTGCACTTGTCCCGGCCTGTAG76410.12475330161139274No Hit
TGTCAGTTTGTCAAATACCCCAA75760.12369205771599415No Hit
AGCAGCATTGTACAGGGCTATG75720.12362675039935422No Hit
CACTAGATTGTGAGCTCCTGGAA74560.12173283821679678No Hit
TTCACAGTGGCTAAGTTCCGC73700.12032873090903867No Hit
TTCACAGTGGCTAAGTTCTGC73330.11972463823011947No Hit
CCCATAAAGTAGAAAGCACTA73190.11949606262187978No Hit
AGAGGTAGTAGGTTGCATAGTT72290.11802664799748173No Hit
TTCACAGTGGCTAAGTTCTG71520.11676948215216343No Hit
TAGCAGCACGTAAATATTGGCGT71460.11667152117720357No Hit
AACATTCAACGCTGTCGGTGA71120.11611640898576431No Hit
CAAAGTGCTGTTCGTGCAGGTAG71060.11601844801080444No Hit
CTCCCACTGCTTCACTTGACTAGCCT69950.11420616997404687No Hit
ACCGGGTGCTGTAGGCTTA67670.11048365292557186No Hit
AACATTCAACGCTGTCGGTGAGA67560.11030405780481212No Hit
AATGACACGATCACTCCCGTTG64970.10607540905237778No Hit
ACTGGACTTGGAGTCAGAAGGCT63040.102924331024502No Hit
TGTAAACATCCCCGACTGGAAGC61650.10065490177126504No Hit
CCTCGACACAAGGGTTTGT61350.1001650968964657No Hit
TCGTTTCCCGGGCGGCGCACCA61330.10013244323814575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)