FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7302664
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6708069.185771110378349No Hit
CCCCCCACTGCTAAATTTGACTG3510834.8076017190439No Hit
AAGCTGCCAGTTGAAGAACTGT3310304.533003298522293No Hit
AACATTCAACGCTGTCGGTGAGT3000924.109349683896178No Hit
TAGCAGCACGTAAATATTGGCG2456363.363649210753774No Hit
TTCAAGTAATCCAGGATAGGCT2444053.3467923486552302No Hit
AACATTCAACGCTGTCGGTGAG1574002.155377818286587No Hit
TAGCTTATCAGACTGATGTTGAC1279131.7515936649967738No Hit
CCCCCACTGCTAAATTTGACTG1075971.4733938190227567No Hit
TAGCTTATCAGACTGATGTTGACT1036741.4196736971603787No Hit
TATTGCACTTGTCCCGGCCTGTAA1018751.3950388515752608No Hit
TATTGCACTTGTCCCGGCCTGTAT921871.2623749360507344No Hit
TGTAAACATCCTTGACTGGAAGCT788031.07909935333188No Hit
TAGCTTATCAGACTGATGTTGA681780.933604503781086No Hit
TATTGCACTTGTCCCGGCCTGT644070.8819658141193405No Hit
AGCAGCATTGTACAGGGCTATGA624400.8550304382072077No Hit
TGAGGTAGTAGGTTGTATAGTT597370.8180165484814856No Hit
CCCATAAAGTAGAAAGCACTAC541230.7411404933870708No Hit
CAACGGAATCCCAAAAGCAGCTGT510190.6986354568688906No Hit
CATAAAGTAGAAAGCACTACT464710.6363568144447013No Hit
TTAATGCTAATCGTGATAGGGGTT458280.6275518084907097No Hit
TGTAAACATCCCCGACTGGAAGCT447690.6130502512507764No Hit
TATTGCACTTGTCCCGGCCTGTA400660.548649095727258No Hit
ACTGGACTTGGAGTCAGAAGGC388440.5319154763248043No Hit
TGTAAACATCCTACACTCTCAGCT375920.5147710479353836No Hit
TGAGGTAGTAGATTGTATAGTT375700.5144697880116078No Hit
CGCCTGTCTGAGCGTCGCT355990.4874796375678793No Hit
TTCAAGTAATTCAGGATAGGTT350310.4797016540813051No Hit
AACCGGGCGGAAACACCA320190.4384564317898235No Hit
TGTCAGTTTGTCAAATACCCCAA316260.4330748340605565No Hit
GCCTGTCTGAGCGTCGCT307990.4217501996531677No Hit
CTACGCCTGTCTGAGCGTCGCT284660.389802954100038No Hit
CCCCCACTGCTAAATTTGACTGGCTT272210.3727543811409097No Hit
CCCATAAAGTAGAAAGCACT266590.3650585594517288No Hit
AACATTCAACGCTGTCGGTGAGTT249790.3420532561815797No Hit
TAGCAGCACATCATGGTTTACA218490.2991921852080282No Hit
TACGCCTGTCTGAGCGTCGCT205550.2814726242368538No Hit
TTCTCACTACTGCACTTGACTAG203130.27815876507532045No Hit
CCCCCCACTGCTAAATTTGACTGGCT198650.27202401753661404No Hit
CATTGCACTTGTCTCGGTCTGA198570.2719144684734229No Hit
CAAAGAATTCTCCTTTTGGGCT184590.25277076968076306No Hit
TGTCAGTTTGTCAAATACCCCA184070.25205870077002035No Hit
TGTAAACATCCTACACTCAGCT179350.2455953060417404No Hit
TCCACCACGTTCCCGTGG176740.24202126785512795No Hit
TGAGGTAGTAGTTTGTACAGTT172680.23646165289817525No Hit
CTCCCACTGCTTCACTTGACTAG172510.23622886113889396No Hit
TCGTTTCCCGGCCAATGCACCA171490.23483211058320635No Hit
TAGCTTATCAGACTGATGTTGACA170020.2328191465470683No Hit
TAGCAGCACGTAAATATTGGC165170.22617773459110263No Hit
CTTCTCACTACTGCACTTGACTAG163340.22367179977060428No Hit
TTCACAGTGGCTAAGTTCCGC144590.19799623808517No Hit
CAACGGAATCCCAAAAGCAGCT141340.1935458073930281No Hit
CCCCCCACTGCTAAATTTGACTGG139650.19123158343311428No Hit
AGCTCGGTCTGAGGCCCCTCAGT139300.19075230628165285No Hit
ATCACATTGCCAGGGATTTCC138890.190190867332798No Hit
TGTAAACATCCCCGACTGGAAGC137270.18797249880317649No Hit
GTCAGGATGGCCGAGCGGTCTAAGG137200.1878766433728842No Hit
TTCAAGTAATCCAGGATAGGCTT135200.18513791679310454No Hit
ACGCCTGTCTGAGCGTCGCT130960.17933181644397167No Hit
TAGCAGCACGTAAATATTGGCGT130120.17818155128046423No Hit
AACGGAATCCCAAAAGCAGCTG129590.1774557887368226No Hit
ATCACATTGCCAGGGATTTCCA129550.17740101420522703No Hit
CACCCGGCTGTGTGCACATGTGC122670.167979794770785No Hit
CAAAGAATTCTCCTTTTGGGCTT122500.16774700301150375No Hit
TATTGCACTTGTCCCGGCCTGTT121090.16581620077275908No Hit
ACTGGACTTGGAGTCAGAAGGCA119100.16309116782587835No Hit
GGAATACCGGGTGCTGTAGGCTTT118040.16163964273859513No Hit
TGAGGTAGTAGTTTGTGCTGTT117600.1610371228910436No Hit
AGCAGCATTGTACAGGGCTATGT117300.16062631390407664No Hit
TGAGAACTGAATTCCATGGGTT116720.15983208319594056No Hit
TATTGCACTTGTCCCGGCCTGTAG115550.15822992814676945No Hit
TTCAAGTAATCCAGGATAGGC107030.14656295291690813No Hit
TTCACAGTGGCTAAGTTCCG106000.1451525087283216No Hit
TCTCCCAACCCTTGTACCAGTGA103770.14209882859186732No Hit
GCGTTCGCGCTTTCCCCTG103350.14152369601011358No Hit
CTCCCACTGCTTCACTTGACTAGCCT102020.1397024428345601No Hit
ACTGGACTTGGAGTCAGAAGGCT101560.13907253572121078No Hit
TTCACAGTGGCTAAGTTCTGC101330.13875758216453613No Hit
TGAGGTAGTAGGTTGTGTGGTT100480.13759362336812977No Hit
CCAAGTCCTTCTGATCGAGGCCC99430.13615579191374544No Hit
AACATTCAACGCTGTCGGTGAGA95050.130157980704028No Hit
TCGTTTCCCGGGCGGCGCACCA94100.12885708557863268No Hit
CAACACCAGTCGATGGGCTGT92240.1263100698594376No Hit
TCTCCCAACCCTTGTACCAGTG91880.12581709907507727No Hit
CAAAGTGCTGTTCGTGCAGGTAG90630.12410539496271497No Hit
TATTGCACTTGTCCCGGCCTGTAAT90510.12394107136792819No Hit
ACCACAGGGTAGAACCACGGAC89940.123160534292691No Hit
GGGCTACGCCTGTCTGAGCGTCGCT89350.12235260995165599No Hit
TCAGGCTCAGTCCCCTCCCGAT89190.12213351182527363No Hit
ACCCCCCACTGCTAAATTTGACTG87930.12040811408001245No Hit
TGAGAACTGAATTCCATAGGCTGT87060.1192167680178083No Hit
AACATTCAACGCTGTCGGTGA85600.11721749761456914No Hit
TATTGCACTTGTCCCGGCCTGTTT84240.11535516354031898No Hit
AGCAGCATTGTACAGGGCTATG84220.11532777627452119No Hit
CACTAGATTGTGAGCTCCTGGAA84190.11528669537582449No Hit
CACTAGATTGTGAGCTCCTGGA83360.11415012384521593No Hit
GGAATACCGGGTGCTGTAGGCTT82580.11308202047910189No Hit
ATCCCACCGCTGCCACCA82130.11246580699865145No Hit
TTCACCACCTTCTCCACCCAGC81700.11187698078399883No Hit
CCCATAAAGTAGAAAGCACTA79730.10917933510291586No Hit
ACTGGACTTGGAGTCAGAAGG78710.10778258454722824No Hit
AGAGGTAGTAGGTTGCATAGTT76750.10509863249904418No Hit
CCCCCACTGCTAAATTTGACTGGCTA76750.10509863249904418No Hit
TTATAAAGCAATGAGACTGATT74740.10234621228636563No Hit
TATTGCACTTGTCCCGGCCTGTAAA74190.10159306247692623No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT73920.10122333438865598No Hit
TGTCAGTTTGTCAAATACCCC73040.10001829469355292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)