FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11178936
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG229906520.566044925921393No Hit
CCCCCCACTGCTAAATTTGACTG6923116.1929954693362586No Hit
TTCAAGTAATCCAGGATAGGCT3463203.097969252172121No Hit
AACATTCAACGCTGTCGGTGAG3117002.7882796716968414No Hit
CCCCCACTGCTAAATTTGACTG2919462.6115723356856146No Hit
TAGCAGCACGTAAATATTGGCG2691982.4080824865622272No Hit
TCGTACGACTCTTAGCGG2323782.078713036732655No Hit
AAGCTGCCAGTTGAAGAACTGT1968271.7606952933624451No Hit
TAGCTTATCAGACTGATGTTGAC1900501.7000723503560626No Hit
TATTGCACTTGTCCCGGCCTGTAA1424951.2746740834727026No Hit
TATTGCACTTGTCCCGGCCTGT1271101.137049178920069No Hit
TGAGGTAGTAGATTGTATAGTT1258521.1257958718074779No Hit
TATTGCACTTGTCCCGGCCTGTAT1241821.1108570618885376No Hit
TGAGAACTGAATTCCATGGGTT1215831.087607979864989No Hit
TAGCTTATCAGACTGATGTTGACT1208661.0811941315345217No Hit
TAGCTTATCAGACTGATGTTGA1111600.9943701261014465No Hit
TTAATGCTAATCGTGATAGGGGTT1016810.9095767253699278No Hit
TGAGGTAGTAGGTTGTATAGTT994880.8899594737817625No Hit
CAACGGAATCCCAAAAGCAGCTGT990880.8863813157173455No Hit
AACATTCAACGCTGTCGGTGAGT983380.879672269346564No Hit
TATTGCACTTGTCCCGGCCTGTA766880.6860044641100012No Hit
CAACGGAATCCCAAAAGCAGCT763890.6833297909568495No Hit
TGTAAACATCCTTGACTGGAAGCT754710.6751179181990128No Hit
AGCAGCATTGTACAGGGCTATGA715870.6403740033935251No Hit
TGAGAACTGAATTCCATAGGCTGT603340.5397114716463177No Hit
TTCAAGTAATTCAGGATAGGTT520720.4658046168257874No Hit
CCCCCACTGCTAAATTTGACTGGCTT509970.45618831702766705No Hit
CCCATAAAGTAGAAAGCACTAC507490.4539698590277285No Hit
CCCATAAAGTAGAAAGCACT466100.4169448684561751No Hit
CATTGCACTTGTCTCGGTCTGA459870.41137188727084584No Hit
CATAAAGTAGAAAGCACTACT459180.41075465500473396No Hit
TGAGGTAGTAGTTTGTACAGTT454930.406952862061291No Hit
TCGTTTCCCGGCCAATGCACCA430500.385099261682865No Hit
TGTAAACATCCCCGACTGGAAGCT422640.3780681810862858No Hit
TTCTCACTACTGCACTTGACTAG411940.36849660826397074No Hit
AACGGAATCCCAAAAGCAGCTG379660.33962087268412666No Hit
CACCCGGCTGTGTGCACATGTGC360720.32267829424911276No Hit
ACTGGACTTGGAGTCAGAAGGC352100.3149673636202944No Hit
CTCCCACTGCTTCACTTGACTAG319210.2855459589356268No Hit
TGATGTAGTAGATTGTATAGTT315620.28233456207281266No Hit
CAAAGAATTCTCCTTTTGGGCT295660.2644795533313725No Hit
TAGCAGCACGTAAATATTGGC281460.2517770922026927No Hit
TAGCTTATCAGACTGATGTTGACA275300.24626672878349065No Hit
CCCATAAAGTAGAAAGCACTA272710.24394987143678076No Hit
TGATAACTGAATTCCATGGGTT271990.24330580298518573No Hit
CTTCTCACTACTGCACTTGACTAG270610.2420713384529619No Hit
TGAGGTAGTAGTTTGTGCTGTT256500.2294493858807314No Hit
TGTAAACATCCTACACTCTCAGCT251890.22532555871149096No Hit
AACCGGGCGGAAACACCA246350.22036980979227358No Hit
TGATGTAGTAGGTTGTATAGTT244940.2191085090745667No Hit
CTCCCACTGCTTCACTTGACTAGCCT233260.20866028752646945No Hit
CACTAGATTGTGAGCTCCTGGA211520.18921299844636377No Hit
GCCTGTCTGAGCGTCGCT209310.18723606611577345No Hit
TATTGCACTTGTCCCGGCCTGTT206340.1845792837529439No Hit
AGCTCGGTCTGAGGCCCCTCAGT201280.1800529138014566No Hit
CACTAGATTGTGAGCTCCTGGAA200860.17967720720469282No Hit
CCCCCCACTGCTAAATTTGACTGGCT198480.1775482031563648No Hit
TCGTTTCCCGGCCAACGCACCA196350.17564283398706282No Hit
TGAGAACTGAATTCCATGGGTTG183530.1641748373906068No Hit
TGTCAGTTTGTCAAATACCCCA183300.1639690933019028No Hit
CCCCCCACTGCTAAATTTGACTGG183290.16396014790674177No Hit
ACCCCCCACTGCTAAATTTGACTG179970.16099027671327576No Hit
GGAATACCGGGTGCTGTAGGCTTT179680.16073086025360553No Hit
TTCACAGTGGCTAAGTTCCGC174820.15638339820533903No Hit
AATGCACCCGGGCAAGGATTCT166980.149370208399082No Hit
CAACACCAGTCGATGGGCTGT166620.14904817417328448No Hit
TATTGCACTTGTCCCGGCCTGTAG166410.14886032087490259No Hit
ATCACATTGCCAGGGATTTCC164830.14744694843945794No Hit
ATGCACCTGGGCAAGGATTCTGA159200.1424106909637912No Hit
TTAATGCTAATCGTGATAGGGGTTT155760.1393334750283927No Hit
TTCACAGTGGCTAAGTTCTGC150660.13477132349626117No Hit
CAAAGTGCTGTTCGTGCAGGTAG146750.1312736739882937No Hit
AGCTACATTGTCTGCTGGGTTT146550.13109476608507287No Hit
ACTGGACTTGGAGTCAGAAGGCT142880.1278118060609704No Hit
TGTAAACATCCCCGACTGGA141980.12700672049647657No Hit
TTCAAGTAATCCAGGATAGGC141290.1263894882303647No Hit
TGATAACTGAATTCCATAGGCTGT138300.12371481507721306No Hit
CAAAGAATTCTCCTTTTGGGC135670.12136217614985899No Hit
GGAATACCGGGTGCTGTAGGCTT135080.1208343978353575No Hit
TTAATGCTAATCGTGATAGGGGT132360.11840125035155404No Hit
CAAAGAATTCTCCTTTTGGGCTT132310.11835652337574881No Hit
ACTGGACTTGGAGTCAGAAGGCA130540.11677318843224437No Hit
TGAGGTAGTAGATTGTATAGT129910.11620962853709871No Hit
AGCAGCATTGTACAGGGCTATGT128320.114787310706493No Hit
TTCACAGTGGCTAAGTTCTG128060.11455473043230589No Hit
TAGCAGCACATCATGGTTTACA127770.11429531397263568No Hit
TTCAAGTAATCCAGGATAGGCTT125330.11211263755334139No Hit
AATCCTTGGAACCTAGGTGTGAGT118850.10631602148898607No Hit
CAACGGAATCCCAAAAGCAGCTGA117290.1049205398438635No Hit
TGTCAGTTTGTCAAATACCCCAA114480.10240688380361064No Hit
TATTGCACTTGTCCCGGCCTGTAAT114230.1021832489245846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)