FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12788052
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG138935810.864500707379046No Hit
CCCCCCACTGCTAAATTTGACTG10144517.932803213499601No Hit
TTCAAGTAATCCAGGATAGGCT4235973.3124435214996No Hit
AACATTCAACGCTGTCGGTGAGT4134183.232845784486957No Hit
TAGCAGCACGTAAATATTGGCG3450612.698307764153602No Hit
CCCCCACTGCTAAATTTGACTG3035932.3740363270340157No Hit
AACATTCAACGCTGTCGGTGAG2827972.2114157809180006No Hit
AAGCTGCCAGTTGAAGAACTGT2819302.2046360149301862No Hit
TATTGCACTTGTCCCGGCCTGTAA2160911.6897882492188803No Hit
TATTGCACTTGTCCCGGCCTGTAT1979281.5477572346437127No Hit
TAGCTTATCAGACTGATGTTGAC1548281.210723885076476No Hit
TAGCTTATCAGACTGATGTTGACT1541921.2057504927255536No Hit
TAGCTTATCAGACTGATGTTGA1141070.8922938380294356No Hit
AGCAGCATTGTACAGGGCTATGA1021780.7990114522524618No Hit
TATTGCACTTGTCCCGGCCTGT924100.7226276527496135No Hit
CCCATAAAGTAGAAAGCACTAC921290.7204302891480266No Hit
CAACGGAATCCCAAAAGCAGCTGT857660.6706729062409192No Hit
ACTGGACTTGGAGTCAGAAGGC846940.6622900813978548No Hit
TGTAAACATCCTTGACTGGAAGCT825700.6456808276976039No Hit
TATTGCACTTGTCCCGGCCTGTA786310.6148786382789184No Hit
CATAAAGTAGAAAGCACTACT771830.6035555689013463No Hit
TGAGGTAGTAGGTTGTATAGTT740140.5787746249389665No Hit
CCCCCACTGCTAAATTTGACTGGCTT735320.575005481679305No Hit
TTCAAGTAATTCAGGATAGGTT635790.49717501930708446No Hit
CTCCCACTGCTTCACTTGACTAG631260.49363265022694625No Hit
TGTAAACATCCCCGACTGGAAGCT617560.48291952519429854No Hit
TCGTACGACTCTTAGCGG564310.4412790939542629No Hit
CCCATAAAGTAGAAAGCACT552810.432286324766274No Hit
CAACGGAATCCCAAAAGCAGCT528940.4136204638517266No Hit
TGAGGTAGTAGATTGTATAGTT513740.4017343689249935No Hit
CCCCCCACTGCTAAATTTGACTGGCT489770.38299031001750694No Hit
AACCGGGCGGAAACACCA476360.3725039591643825No Hit
CAAAGAATTCTCCTTTTGGGCT457350.3576385207066721No Hit
CATTGCACTTGTCTCGGTCTGA454280.3552378423234438No Hit
CTTCTCACTACTGCACTTGACTAG447540.3499672975993529No Hit
TTCTCACTACTGCACTTGACTAG444590.3476604568076514No Hit
TGTAAACATCCTACACTCTCAGCT432670.3383392560493186No Hit
AGCTCGGTCTGAGGCCCCTCAGT400450.31314386272436173No Hit
CACTAGATTGTGAGCTCCTGGAA381470.29830188366453314No Hit
CACCCGGCTGTGTGCACATGTGC375550.2936725624825423No Hit
AACATTCAACGCTGTCGGTGAGTT361980.2830610948407154No Hit
CACTAGATTGTGAGCTCCTGGA334280.26140025079660295No Hit
CTACGCCTGTCTGAGCGTCGCT327980.2564737772414438No Hit
TCGTTTCCCGGCCAATGCACCA313830.24540876124057048No Hit
GCCTGTCTGAGCGTCGCT306300.23952045237226124No Hit
GGAATACCGGGTGCTGTAGGCTTT293450.22947201027959538No Hit
CGCCTGTCTGAGCGTCGCT286070.22370099840069466No Hit
ACTGGACTTGGAGTCAGAAGGCT282800.22114392403158822No Hit
CAAAGAATTCTCCTTTTGGGCTT272540.21312080995604335No Hit
CCCCCCACTGCTAAATTTGACTGG266890.2087026233549879No Hit
TTCAAGTAATCCAGGATAGGCTT263050.20569982042612903No Hit
AACGGAATCCCAAAAGCAGCTG262680.20541048785225458No Hit
TATTGCACTTGTCCCGGCCTGTAG257470.2013363724201309No Hit
CGGGCCGCCGGTGAAATACCACTACT256560.2006247706843857No Hit
TCGTTTCCCGGGCGGCGCACCA254370.19891223463902086No Hit
ACTGGACTTGGAGTCAGAAGGCA252840.19771580534705363No Hit
CTCCCACTGCTTCACTTGACTAGCCT241780.18906710732799648No Hit
CAACACCAGTCGATGGGCTGT222090.17366992251830068No Hit
ACCCCCCACTGCTAAATTTGACTG222030.17362300372253725No Hit
AGCAGCATTGTACAGGGCTATGT219270.1714647391174199No Hit
TAGCAGCACGTAAATATTGGC215390.1684306569913854No Hit
CCCATAAAGTAGAAAGCACTA215140.1682351620090378No Hit
TATTGCACTTGTCCCGGCCTGTT214380.16764085726270114No Hit
TGTCAGTTTGTCAAATACCCCAA210570.16466151373172394No Hit
TGTAAACATCCCCGACTGGAAGC205710.1608610912748869No Hit
TTCAAGTAATCCAGGATAGGC201570.15762369436721088No Hit
TATTGCACTTGTCCCGGCCTGTTT198810.15546542976209357No Hit
TAGCTTATCAGACTGATGTTGACA198110.15491804381152033No Hit
TTCACAGTGGCTAAGTTCCG196790.15388583030472505No Hit
GGAATACCGGGTGCTGTAGGCTT196060.1533149849562701No Hit
CCCCCACTGCTAAATTTGACTGGCTA194160.15182922309042848No Hit
TACGCCTGTCTGAGCGTCGCT191630.1498508138690709No Hit
TATTGCACTTGTCCCGGCCTGTAAT189180.14793496304206458No Hit
ACTGGACTTGGAGTCAGAAGG183650.143610614032536No Hit
TGAGGTAGTAGTTTGTACAGTT176580.1380820159317463No Hit
CCCCCCACTGCTAAATTTGACTGTA175950.13758936857623036No Hit
GCGGGCCGCCGGTGAAATACCACTAC171920.1344379894607873No Hit
TATTGCACTTGTCCCGGCCTGTAAA170820.1335778115384579No Hit
CCCCCACTGCTAAATTTGACTGGCT167400.13090344017994296No Hit
GACCCGCGGGCGCTCTCCAGTCCT163460.12782243925814504No Hit
TGTCAGTTTGTCAAATACCCCA161140.12600824582195944No Hit
GAATACCGGGTGCTGTAGGCTTT156820.12263009252699317No Hit
ATCACATTGCCAGGGATTTCC156520.12239549854817607No Hit
ATACCGGGTGCTGTAGGCTTA156320.122239102562298No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC151600.11854815729557559No Hit
CAACGGAATCCCAAAAGCAGCTGA147410.11527166139143007No Hit
ACCACAGGGTAGAACCACGGAC146550.11459915865215436No Hit
CCCCCCACTGCTAAATTTGACTGT146000.1141690696909897No Hit
ATGCACCTGGGCAAGGATTCTGA144780.11321505417713348No Hit
ATCACATTGCCAGGGATTTCCA142190.11118972616001249No Hit
ACTCCCACTGCTTCACTTGACTAG142100.11111934796636735No Hit
TGAGGTAGTAGTTTGTGCTGTT140140.10958666730476228No Hit
TTCACAGTGGCTAAGTTCTGC137110.10721726811870956No Hit
CTACGGGGATGATTTTACGAACTGAA133900.10470711254536656No Hit
TGAGGGGCAGAGAGCGAGACTTT133790.10462109475313362No Hit
TTAATGCTAATCGTGATAGGGGTT133220.10417536619338115No Hit
CAAAGTGCTGTTCGTGCAGGTAG131230.10261922613389435No Hit
TTCACAGTGGCTAAGTTCTG130670.10218131737343578No Hit
AGCAGCATTGTACAGGGCTATG130610.10213439857767236No Hit
AACATTCAACGCTGTCGGTGAGA129260.10107872567299539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)