FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003947191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22663772
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG230423610.167045450333687No Hit
CAACGGAATCCCAAAAGCAGCTG18836078.31109225772303No Hit
CCCCCACTGCTAAATTTGACTG8645513.8146827456612256No Hit
TTCAAGTAATCCAGGATAGGCT8635633.8103233654133124No Hit
AACATTCAACGCTGTCGGTGAGT8029083.5426935992825905No Hit
TAGCAGCACGTAAATATTGGCG6324662.790647558579393No Hit
AACATTCAACGCTGTCGGTGAG5964592.6317728575808124No Hit
AAGCTGCCAGTTGAAGAACTGT5850182.581291410803109No Hit
TGAGGTAGTAGGTTGTATAGTT4826762.1297249195764945No Hit
TAGCTTATCAGACTGATGTTGA4109661.8133168653479217No Hit
TAGCTTATCAGACTGATGTTGAC4053081.7883519124707044No Hit
TAGCTTATCAGACTGATGTTGACT3739431.6499592389122166No Hit
TTCAAGTAATTCAGGATAGGTT2845591.255567696321689No Hit
TGAGGTAGTAGATTGTATAGTT2596171.1455154067028206No Hit
GCCTGTCTGAGCGTCGCT2577091.137096684523653No Hit
TATTGCACTTGTCCCGGCCTGT2169950.9574531547528804No Hit
TATTGCACTTGTCCCGGCCTGTAA2086330.9205572664603227No Hit
TATTGCACTTGTCCCGGCCTGTAT2033950.8974454914212868No Hit
TCGTACGACTCTTAGCGG1831820.8082591017947057No Hit
TGTAAACATCCTTGACTGGAAGCT1470040.6486298926762941No Hit
AGCAGCATTGTACAGGGCTATGA1353240.5970938994621019No Hit
CATAAAGTAGAAAGCACTACT1340840.5916226125112801No Hit
TATTGCACTTGTCCCGGCCTGTA1285030.566997408904396No Hit
GGAATACCGGGTGCTGTAGGCTTT1178500.5199928767373763No Hit
CATTGCACTTGTCTCGGTCTGA1153850.5091164877585249No Hit
CAACACCAGTCGATGGGCTGT1098730.48479573479648486No Hit
ACTGGACTTGGAGTCAGAAGGC1084640.4785787643822043No Hit
CGCCTGTCTGAGCGTCGCT1074150.47395023211493653No Hit
TGTAAACATCCCCGACTGGAAGCT1028760.4539226744780172No Hit
TGAGGTAGTAGTTTGTACAGTT975260.43031671868213284No Hit
GGAATACCGGGTGCTGTAGGCTT928950.409883226852088No Hit
CAACGGAATCCCAAAAGCAGCTGT903240.39853913108550515No Hit
CTCCCACTGCTTCACTTGACTAG895230.3950048562084017No Hit
CCCATAAAGTAGAAAGCACT876080.38655524773193095No Hit
TGATGTAGTAGGTTGTATAGTT773980.34150537695137423No Hit
CACCCGGCTGTGTGCACATGTGC694050.30623763775950447No Hit
TTCTCACTACTGCACTTGACTAG687460.30332991348483385No Hit
TGAGGTAGTAGTTTGTGCTGTT651240.28734846079461085No Hit
CCCATAAAGTAGAAAGCACTAC519680.2292998711776663No Hit
TTCAAGTAATCCAGGATAGGCTT517160.2281879644747573No Hit
TAGCAGCACGTAAATATTGGC516250.22778644260981798No Hit
CCCCCCACTGCTAAATTTGACTGG503090.22197981871684905No Hit
AACGGAATCCCAAAAGCAGCTG476240.2101327175370455No Hit
AACATTCAACGCTGTCGGTGAGTT472760.20859722732826644No Hit
TTCAAGTAATCCAGGATAGGC464720.20504971546660455No Hit
CACTAGATTGTGAGCTCCTGGA463680.20459083333524533No Hit
AACCGGGCGGAAACACCA460460.20317006365930612No Hit
CTTCTCACTACTGCACTTGACTAG457250.2017537063115531No Hit
TGAGGTAGTAGGTTGTGTGGTT455000.2007609324696701No Hit
CCCCCCACTGCTAAATTTGACTGGCT454090.20035941060473078No Hit
AGCTCGGTCTGAGGCCCCTCAGT437930.19322908825591786No Hit
TAGCTTATCAGACTGATGTTGACA437480.19303053348754126No Hit
TGTAAACATCCTACACTCTCAGCT428610.18911679838642922No Hit
CTCCCACTGCTTCACTTGACTAGCCT426340.18811519988817396No Hit
TGAGGTAGTAGGTTGTATAGT419060.18490302496865924No Hit
TGATGTAGTAGATTGTATAGTT416790.18390142647040397No Hit
CAACGGAATCCCAAAAGCAGCT414340.18282040606479805No Hit
TTATAAAGCAATGAGACTGATT412420.1819732390530579No Hit
TGAGAACTGAATTCCATAGGCTGT401300.17706673011006288No Hit
CCCCCACTGCTAAATTTGACTGGCTT395880.17467524823317143No Hit
TATTGCACTTGTCCCGGCCTGTT375820.16582411789176135No Hit
TGTCAGTTTGTCAAATACCCCAA369890.16320760727737643No Hit
AGCAGCATTGTACAGGGCTATGT367930.16234279095289167No Hit
TGTCAGTTTGTCAAATACCCCA352030.15532718913691862No Hit
CCCGCCACTGCTAAATTTGACTG325660.14369187971005004No Hit
CACTAGATTGTGAGCTCCTGGAA320040.14121215126943565No Hit
TGAGAACTGAATTCCATGGGTT319280.1408768143272885No Hit
TTCACAGTGGCTAAGTTCTG316970.13985756651628864No Hit
ACCCCCCACTGCTAAATTTGACTG316490.1396457747633536No Hit
TGAGGTAGTAAGTTGTATTGTT310050.13680423541147518No Hit
ACCGGGTGCTGTAGGCTT304980.13456718502109888No Hit
CAAAGAATTCTCCTTTTGGGCT296100.1306490375918007No Hit
TTCACAGTGGCTAAGTTCCGC282020.12443647950570629No Hit
CTACGCCTGTCTGAGCGTCGCT277770.12256124002659398No Hit
AGCAGCATTGTACAGGGCTATG274870.12128166485261148No Hit
AACATTCAACGCTGTCGGTGA274190.1209816265359535No Hit
ACCACAGGGTAGAACCACGGAC273470.12066393890655094No Hit
TGAGGTAGTAGATTGTATAGT262690.11590744912188491No Hit
CAAAGTGCTGTTCGTGCAGGT261380.11532943412949971No Hit
CAAGGGAATCCCAAAAGCAGCTG258430.11402779731458648No Hit
GACCCGCGGGCGCTCTCCAGTCCT255110.1125629043567858No Hit
TGTAAACATCCCCGACTGGAAGC253920.11203783730263435No Hit
TATTGCACTTGTCCCGGCCTGTAG253860.11201136333351747No Hit
ACCGGGTGCTGTAGGCTTT250430.11049793476566919No Hit
TAGCTTATCAGACTGATGTTG248440.10961988145662602No Hit
AGAGGTAGTAGGTTGCATAGTT246820.10890508429047027No Hit
TCGTTTCCCGGCCAATGCACCA244540.10789907346402884No Hit
CAAAGAATTCTCCTTTTGGGCTT243790.10756814885006785No Hit
GGGAATACCGGGTGCTGTAGGCTTT243430.10740930503536658No Hit
ACTGGACTTGGAGTCAGAAGGCT239960.10587822715477371No Hit
ATCCCGGACGAGCCCCCA237690.10487662865651844No Hit
TTCAAGTAATTCAGGATAGGT232160.10243661116957935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)