FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8060032
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6488398.050079701916816No Hit
CAACGGAATCCCAAAAGCAGCTG5573536.915022173609237No Hit
AACATTCAACGCTGTCGGTGAGT4095275.080959976337563No Hit
TAGCAGCACGTAAATATTGGCG3203993.9751579150057967No Hit
TTCAAGTAATCCAGGATAGGCT3132783.886808389842621No Hit
AAGCTGCCAGTTGAAGAACTGT2899993.597988196572917No Hit
AACATTCAACGCTGTCGGTGAG2399242.9767127475424418No Hit
GGAATACCGGGTGCTGTAGGCTTT2282062.8313287093649255No Hit
CCCCCACTGCTAAATTTGACTG2007192.490300286648986No Hit
GGAATACCGGGTGCTGTAGGCTT1780212.2086885014848576No Hit
TATTGCACTTGTCCCGGCCTGTAT1354141.680067771443091No Hit
TATTGCACTTGTCCCGGCCTGTAA1307691.6224377272943828No Hit
TAGCTTATCAGACTGATGTTGACT1298721.6113087392208865No Hit
TAGCTTATCAGACTGATGTTGA1113111.3810242937000747No Hit
TGAATACCGGGTGCTGTAGGCTTT963611.1955411591418No Hit
TAGCTTATCAGACTGATGTTGAC953561.1830722260159763No Hit
TGAGGTAGTAGGTTGTATAGTT788420.9781847019962203No Hit
TATTGCACTTGTCCCGGCCTGT785780.9749092807571981No Hit
TGAATACCGGGTGCTGTAGGCTT751040.9318077149073354No Hit
CATAAAGTAGAAAGCACTACT596980.7406670345725674No Hit
TGTAAACATCCTTGACTGGAAGCT564710.700629972684972No Hit
TTCTCACTACTGCACTTGACTAG488770.606411984468548No Hit
TGAGGTAGTAGATTGTATAGTT461910.5730870547412219No Hit
GCCTGTCTGAGCGTCGCT442520.5490300782924932No Hit
TATTGCACTTGTCCCGGCCTGTA439830.5456926225603075No Hit
TGAGAACTGAATTCCATGGGTT412920.512305658339818No Hit
ACTGGACTTGGAGTCAGAAGGC391270.48544472280010803No Hit
TTCAAGTAATTCAGGATAGGTT387560.48084176340739093No Hit
TGTAAACATCCCCGACTGGAAGCT383470.4757673418666328No Hit
AGCAGCATTGTACAGGGCTATGA374080.4641172640505646No Hit
CAACGGAATCCCAAAAGCAGCTGT362030.44916695119820865No Hit
CTTCTCACTACTGCACTTGACTAG343630.4263382577141133No Hit
GGAATACCGGGTGCTGTAGGCTTA332910.4130380623799012No Hit
CTCCCACTGCTTCACTTGACTAG320670.39785201845352475No Hit
CCCATAAAGTAGAAAGCACT296130.3674054892089758No Hit
CCCATAAAGTAGAAAGCACTAC273900.3398249535485715No Hit
CATTGCACTTGTCTCGGTCTGA268240.3328026489224857No Hit
GGAATACCGGGTGCTGTAGGCT248160.3078895964681034No Hit
TGTAAACATCCTACACTCTCAGCT245790.3049491614921628No Hit
CAACACCAGTCGATGGGCTGT238330.29569361511219805No Hit
TGAGGTAGTAGTTTGTACAGTT226960.28158697136686306No Hit
CCCCCCACTGCTAAATTTGACTGG220980.2741676459845321No Hit
CACCCGGCTGTGTGCACATGTGC216790.2689691554574473No Hit
AACATTCAACGCTGTCGGTGAGTT216410.2684976933094062No Hit
AGCTCGGTCTGAGGCCCCTCAGT185850.23058221108799565No Hit
CCCCCCACTGCTAAATTTGACTGGCT184820.22930430052883166No Hit
TATTGCACTTGTCCCGGCCTGTAG177250.21991227826390763No Hit
AACATTCAACGCTGTCGGTGA172450.2139569669202306No Hit
CTCCCACTGCTTCACTTGACTAGCCT169380.2101480490400038No Hit
TAGCAGCACGTAAATATTGGC166260.2062770966666137No Hit
AACCGGGCGGAAACACCA165350.20514806889104162No Hit
TCCTGTCTGAGCGTCGCT163880.20332425479204055No Hit
TGATGTAGTAGGTTGTATAGTT158450.19658730883450587No Hit
CACTAGATTGTGAGCTCCTGGA158200.19627713636868935No Hit
TGAGGTAGTAGTTTGTGCTGTT154350.19150048039511505No Hit
TGAGAACTGAATTCCATAGGCTGT154320.1914632596992171No Hit
CCCCCACTGCTAAATTTGACTGGCTT152870.1896642593974813No Hit
TTCAAGTAATCCAGGATAGGCTT152220.18885781098635837No Hit
TATTGCACTTGTCCCGGCCTGTT144680.17950300941733235No Hit
CAAAGAATTCTCCTTTTGGGCT142240.17647572615096316No Hit
TGAATACCGGGTGCTGTAGGCTTA140880.17478838793692136No Hit
ACTGGACTTGGAGTCAGAAGGCT131660.16334922739760835No Hit
TGTAAACATCCCCGACTGGAAGC128370.15926735774746303No Hit
AACATTCAACGCTGTCGGTGAGA128330.1592177301529324No Hit
TAGCAGCACATCATGGTTTACA128000.1588083024980546No Hit
CAAAGTGCTGTTCGTGCAGGT124640.15463958455748067No Hit
TTCAAGTAATCCAGGATAGGC123820.1536222188696025No Hit
TTCACAGTGGCTAAGTTCCGC118920.14754283853959885No Hit
AACGGAATCCCAAAAGCAGCTG116430.14445352078006637No Hit
ATCCCGGACGAGCCCCCA116010.14393243103749462No Hit
TGTAAACATCCTACACTCAGCT115860.14374632755800473No Hit
TTCACAGTGGCTAAGTTCCG115370.14313838952500435No Hit
TGAGGTAGTAGGTTGTGTGGTT113610.14095477536565612No Hit
CACTAGATTGTGAGCTCCTGGAA112740.13987537518461465No Hit
GACCCGCGGGCGCTCTCCAGTCCT111730.13862227842271593No Hit
CAAAGAATTCTCCTTTTGGGCTT109650.13604164350712256No Hit
TATTGCACTTGTCCCGGCCTGTTT106980.1327290015722022No Hit
TGAATACCGGGTGCTGTAGGCT105590.13100444266226238No Hit
TTCACAGTGGCTAAGTTCTG102840.12759254553828075No Hit
TAGCTTATCAGACTGATGTTGACA97940.1215131652082771No Hit
TATTGCACTTGTCCCGGCCTGTAAT97890.12145113071511378No Hit
TGTCAGTTTGTCAAATACCCCAA95730.11877124061045911No Hit
ACCACAGGGTAGAACCACGGAC92650.11494991583159968No Hit
ACTGGACTTGGAGTCAGAAGG92050.11420550191364005No Hit
TTCACAGTGGCTAAGTTCTGC91260.11322535692165987No Hit
TGAGAACTGAATTCCATGGGTTG91130.11306406723943527No Hit
TGATGTAGTAGATTGTATAGTT90860.11272908097635344No Hit
AATGACACGATCACTCCCGTTG89240.11071916339786243No Hit
TTAATGCTAATCGTGATAGGGGTT88330.10959013562229034No Hit
ACCCCCCACTGCTAAATTTGACTG85650.1062650867887373No Hit
CCCCCCACTGCTAAATTTGACTGT84360.10466459686512411No Hit
ACTGGACTTGGAGTCAGAAGGCA83860.10404425193349107No Hit
CGCCTGTCTGAGCGTCGCT82320.10213358954406135No Hit
ACTTGACTTGGAGTCAGAAGGC81170.1007067962013054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)