FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8933930
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8859649.91684510624104No Hit
CCCCCCACTGCTAAATTTGACTG7339758.215589331906562No Hit
AACATTCAACGCTGTCGGTGAGT3399873.8055704488394246No Hit
AAGCTGCCAGTTGAAGAACTGT3173223.5518747068759216No Hit
TTCAAGTAATCCAGGATAGGCT2924103.2730276597197427No Hit
TAGCAGCACGTAAATATTGGCG2688473.009280350304961No Hit
CCCCCACTGCTAAATTTGACTG2320602.5975130765519765No Hit
AACATTCAACGCTGTCGGTGAG2249612.518051965932126No Hit
TATTGCACTTGTCCCGGCCTGTAT1833302.052064433009885No Hit
TATTGCACTTGTCCCGGCCTGTAA1796632.011018667036791No Hit
TAGCTTATCAGACTGATGTTGACT1671671.8711474121691125No Hit
TAGCTTATCAGACTGATGTTGAC1434641.6058330432407686No Hit
TAGCTTATCAGACTGATGTTGA1226641.3730127726543637No Hit
TGAGGTAGTAGGTTGTATAGTT1046551.1714329527990481No Hit
TATTGCACTTGTCCCGGCCTGT1009621.1300961614877214No Hit
TGTAAACATCCTTGACTGGAAGCT770320.8622409174909587No Hit
TATTGCACTTGTCCCGGCCTGTA721420.8075057673386741No Hit
TTAATGCTAATCGTGATAGGGGTT657950.7364620049631013No Hit
CATAAAGTAGAAAGCACTACT653690.731693666728976No Hit
TCGTACGACTCTTAGCGG647370.724619512353466No Hit
TGAGGTAGTAGATTGTATAGTT635620.711467405721782No Hit
GCCTGTCTGAGCGTCGCT564960.6323756734158428No Hit
ACTGGACTTGGAGTCAGAAGGC561940.628995302179444No Hit
AGCAGCATTGTACAGGGCTATGA561000.6279431336489093No Hit
CAACGGAATCCCAAAAGCAGCTGT560270.6271260240454088No Hit
TGTAAACATCCCCGACTGGAAGCT507320.5678575945860332No Hit
TTCAAGTAATTCAGGATAGGTT503730.5638392062619698No Hit
CCCATAAAGTAGAAAGCACTAC495840.5550077065748221No Hit
CAACGGAATCCCAAAAGCAGCT425740.4765427980743077No Hit
CCCATAAAGTAGAAAGCACT354880.3972272001235739No Hit
TGAGAACTGAATTCCATGGGTT337170.37740389727701024No Hit
CACCCGGCTGTGTGCACATGTGC330960.3704528690061373No Hit
CATTGCACTTGTCTCGGTCTGA321420.3597744777494339No Hit
TGAGGTAGTAGTTTGTACAGTT311760.34896176710585375No Hit
CGCCTGTCTGAGCGTCGCT297090.3325412220601684No Hit
CCCCCACTGCTAAATTTGACTGGCTT273130.30572211781377284No Hit
CAACACCAGTCGATGGGCTGT255480.28596597466064766No Hit
TCGTTTCCCGGCCAATGCACCA254160.28448846140500317No Hit
TGTAAACATCCTACACTCTCAGCT251810.2818580400786664No Hit
CACTAGATTGTGAGCTCCTGGA241470.27028418624278455No Hit
ATCCCGGACGAGCCCCCA233100.2609154090081297No Hit
AACATTCAACGCTGTCGGTGAGTT224000.25072952216997446No Hit
CACTAGATTGTGAGCTCCTGGAA221540.24797597473900065No Hit
TTCTCACTACTGCACTTGACTAG218110.2441366789307729No Hit
TGAGAACTGAATTCCATAGGCTGT217310.24324121635159443No Hit
TATTGCACTTGTCCCGGCCTGTAG216360.24217785453882No Hit
AACGGAATCCCAAAAGCAGCTG210410.23551785160618005No Hit
CAAAGAATTCTCCTTTTGGGCT205600.23013388284886943No Hit
TATTGCACTTGTCCCGGCCTGTT203050.22727959587773802No Hit
TAGCTTATCAGACTGATGTTGACA198770.22248887107913315No Hit
CCCCCCACTGCTAAATTTGACTGGCT185870.20804953698988016No Hit
TGAGGTAGTAGTTTGTGCTGTT181320.20295659357080253No Hit
ACTGGACTTGGAGTCAGAAGGCT176980.19809870907875926No Hit
TGTCAGTTTGTCAAATACCCCAA175820.19680028833895052No Hit
TTCAAGTAATCCAGGATAGGCTT174470.1952891952365868No Hit
CTCCCACTGCTTCACTTGACTAG173870.19461759830220296No Hit
CTTCTCACTACTGCACTTGACTAG172730.19334156412687362No Hit
TAGCAGCACGTAAATATTGGC163910.18346908919143087No Hit
ACTGGACTTGGAGTCAGAAGGCA161570.1808498611473338No Hit
GGAATACCGGGTGCTGTAGGCTTT157930.17677550641207174No Hit
TATTGCACTTGTCCCGGCCTGTTT156350.17500696781819425No Hit
TCGTTTCCCGGGCGGCGCACCA153340.17163778986403522No Hit
TATTGCACTTGTCCCGGCCTGTAAT153310.171604210017316No Hit
CAAAGAATTCTCCTTTTGGGCTT153000.17125721826788434No Hit
TCCTGTCTGAGCGTCGCT151600.169690158754322No Hit
TTCAAGTAATCCAGGATAGGC146670.1641718706101346No Hit
TGTAAACATCCCCGACTGGAAGC144940.16223543278266117No Hit
ACCCCCCACTGCTAAATTTGACTG137310.1536949584337464No Hit
CACCCGTAGAACCGACCTTGCG136940.15328080699087634No Hit
AGCTCGGTCTGAGGCCCCTCAGT135320.15146749526803993No Hit
CTACGCCTGTCTGAGCGTCGCT133220.14911690599769642No Hit
AGCAGCATTGTACAGGGCTATGT128110.14339713877319388No Hit
TATTGCACTTGTCCCGGCCTGTAAA125570.1405540450843022No Hit
TGAGGTAGTAGGTTGTGTGGTT123960.13875192664370553No Hit
CCCCCCACTGCTAAATTTGACTGG120560.13494621068219698No Hit
ACCACAGGGTAGAACCACGGAC116340.13022264557703048No Hit
CCCATAAAGTAGAAAGCACTA114920.1286331994989887No Hit
CTCCCACTGCTTCACTTGACTAGCCT113850.12743551829933747No Hit
ACTGGACTTGGAGTCAGAAGG112270.12566697970545998No Hit
GGAATACCGGGTGCTGTAGGCTT110830.12405514706293871No Hit
CAACGGAATCCCAAAAGCAGCTGA110620.12382008813590435No Hit
TGTCAGTTTGTCAAATACCCCA110330.12349548295095215No Hit
TTCACAGTGGCTAAGTTCTGC108720.12169336451035546No Hit
TTATAAAGCAATGAGACTGATT105560.11815628732260047No Hit
ACCGGGTGCTGTAGGCTT104520.11699218596966846No Hit
TACGCCTGTCTGAGCGTCGCT103010.11530200035146905No Hit
ACCGGGTGCTGTAGGCTTA101980.11414909228077677No Hit
TTCACAGTGGCTAAGTTCCGC100450.11243652009809793No Hit
TATTGCACTTGTCCCGGCCTGA98200.10991803159415844No Hit
ATGCACCTGGGCAAGGATTCTGA97260.10886586306362374No Hit
AACATTCAACGCTGTCGGTGA95810.10724283713886273No Hit
TTCACAGTGGCTAAGTTCCG95130.10648169394656103No Hit
CAAAGTGCTGTTCGTGCAGGT93780.10497060084419735No Hit
TGAGGTAGTAGGTTGTATAGT92330.10334757491943636No Hit
AACATTCAACGCTGTCGGTGAGA91240.10212750715530568No Hit
TTAATGCTAATCGTGATAGGGGTTT90380.10116488488268881No Hit
GACCCGCGGGCGCTCTCCAGTCCT89990.1007283468753393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)